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PROTEIN-POLYMER SUPRAMOLECULAR ASSEMBLIES IN THE DESIGN OF ANTIOXIDANT NANOREACTORS AND PROCESSORS

English title PROTEIN-POLYMER SUPRAMOLECULAR ASSEMBLIES IN THE DESIGN OF ANTIOXIDANT NANOREACTORS AND PROCESSORS
Applicant Palivan Cornelia
Number 140302
Funding scheme Project funding
Research institution Physikalische Chemie Departement Chemie Universität Basel
Institution of higher education University of Basel - BS
Main discipline Physical Chemistry
Start/End 01.06.2012 - 30.04.2016
Approved amount 215'039.00
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Keywords (5)

antioxidant nanoreactors/processors; self-assembly; vesicles; amphiphilic copolymers; enzymes

Lay Summary (English)

Lead
Lay summary
Efforts to understand the expression of nature’s intelligence and to mimic its structures and functions in the design of new materials and active assemblies have yielded powerful methods in use today in various domains that include chemistry, electronics, materials science and medicine. Of particular interest are polymer supramolecular structures in the form of particles, micelles, vesicles, and films that provide a variety of architectures to allow the insertion/encapsulation/attachment of biomolecules.
In this project, we plan to combine physical chemistry, polymer science and enzymatic reactions in order to obtain a better understanding of the structural conformation of biomolecules - polymer supramolecular assemblies and to develop efficient antioxidant nanoreactors and processors. The necessity to develop an antioxidant platform on the nanoscale originates with the increasing evidence of the toxicity of reactive free radicals species generated by oxidative stress as involved in several severe pathological conditions, such as cancer, degenerative diseases, HIV, or viral infections, and recently associated with inorganic nanoparticles or quantum dots.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Publications

Publication
Active surfaces engineered by immobilizing protein-polymer nanoreactors for selectively detecting sugar alcohols
Zhang Xiaoyan, Lomora Mihai, Einfalt Tomaz, Meier Wolfgang, Klein Noreen, Schneider Dirk, Palivan Cornelia (2016), Active surfaces engineered by immobilizing protein-polymer nanoreactors for selectively detecting sugar alcohols, in Biomaterials, 89, 79-88.
Artificial Organelles: Reactions inside Protein-Polymer Supramolecular Assemblies.
Garni Martina, Einfalt TomaŽ, Lomora Mihai, Car Anja, Meier Wolfgang, Palivan Cornelia G (2016), Artificial Organelles: Reactions inside Protein-Polymer Supramolecular Assemblies., in Chimia, 70(6), 424-7.
Bioinspired polymer vesicles and membranes for biological and medical applications
Palivan Cornelia G, Goers Roland, Najer Adrian, Zhang Xiaoyan, Meier Wolfgang (2016), Bioinspired polymer vesicles and membranes for biological and medical applications, in Chem. Soc. Rev., 45, 377-411.
Bio-nanoreactors: from confined reaction spaces to artificial organelles
Postupalenko Viktoriia, Einfalt Tomaz, Lomora Mihai, Dinu I. Adrian, Palivan G. Cornelia (2016), Bio-nanoreactors: from confined reaction spaces to artificial organelles, in Samahe Sadjadi (ed.), Elsevier, Elsevier, 341-371.
Does Membrane Thickness Affect the Transport of Selective Ions Mediated by Ionophores in Synthetic Membranes?
Lomora Mihai, Dinu Ionel Adrian, Itel Fabian, Rigo Serena, Spulber Mariana, Palivan Cornelia G (2015), Does Membrane Thickness Affect the Transport of Selective Ions Mediated by Ionophores in Synthetic Membranes?, in Macromolecular rapid communications, na.
Mobility of membrane proteins within synthetic polymer membranes
Fabian Itel, Adrian Najer, Cornelia G. Palivan, Wolfgang Meier (2015), Mobility of membrane proteins within synthetic polymer membranes, in Nano Letters, 15(6), 3871-3878.
New concepts to fight oxidative stress: antioxidant compounds inside 3-D nano-assemblies
P. Richard, J. Duskey, S. Stolarov, M. Spulber, C.G. Palivan (2015), New concepts to fight oxidative stress: antioxidant compounds inside 3-D nano-assemblies, in Expert Opinion Drug Delivery, 12(9), 1-19.
Polymeric 3D nano-architectures for transport and delivery of therapeutically relevant biomacromolecules.
Gunkel-Grabole G, Sigg S, Lomora M, Lörcher S, Palivan C G., Meier W P (2015), Polymeric 3D nano-architectures for transport and delivery of therapeutically relevant biomacromolecules., in Biomaterials science, 3(1), 25-40.
Polymersomes with engineered ion selective permeability as stimuli-responsive nanocompartments with preserved architecture.
Lomora Mihai, Garni Martina, Itel Fabian, Tanner Pascal, Spulber Mariana, Palivan Cornelia G (2015), Polymersomes with engineered ion selective permeability as stimuli-responsive nanocompartments with preserved architecture., in Biomaterials, 53, 406-14.
Selective ion-permeable membranes by insertion of biopores into polymersomes.
Lomora Mihai, Itel Fabian, Dinu Ionel Adrian, Palivan Cornelia G (2015), Selective ion-permeable membranes by insertion of biopores into polymersomes., in Physical chemistry chemical physics : PCCP, 17(24), 15538-46.
Aiding nature's organelles: Artificial peroxisomes play their role
Tanner Pascal, Balasubramanian Vimalkumar, Palivan Cornelia G. (2013), Aiding nature's organelles: Artificial peroxisomes play their role, in Nano Letters, 13(6), 2875-2883.
Mimicking the cell membrane with block copolymer membranes
Zhang Xiaoyan, Tanner Pascal, Graff Alexandra, Palivan Cornelia G., Meier Wolfgang (2012), Mimicking the cell membrane with block copolymer membranes, in JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 50(12), 2293-2318.
Polymer nanoreactors with dual functionality: simultaneous detoxification of peroxynitrite and oxygen transport.
Dobrunz Dominik, Toma Adriana C, Tanner Pascal, Pfohl Thomas, Palivan Cornelia G (2012), Polymer nanoreactors with dual functionality: simultaneous detoxification of peroxynitrite and oxygen transport., in Langmuir : the ACS journal of surfaces and colloids, 28(45), 15889-99.
Specific His6-tag attachment to metal-functionalized polymersomes relies on molecular recognition
Tanner Pascal, Ezhevskaya Maria, Nehring Rainer, Van Doorslaer Sabine, Meier Wolfgang, Palivan Cornelia (2012), Specific His6-tag attachment to metal-functionalized polymersomes relies on molecular recognition, in Journal of Physical Chemistry B, 116(33), 10113-10124.
Polymer capsules as micro-/nanoreactors for therapeutic applications: Current strategies to control membrane permeability
Larrañaga Aitor, Lomora Mihai, Sarasua Jose-Ramon, Palivan Cornelia, Pandit Abhay, Polymer capsules as micro-/nanoreactors for therapeutic applications: Current strategies to control membrane permeability, in Progress in Materials Science, 1.

Collaboration

Group / person Country
Types of collaboration
Prof. Sabine Van Doorslaer, Antwerp University Belgium (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Prof. Dirk Schneider, Johannes Gutenberg-University Mainz Germany (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Prof. Abhay Pandit, Network of Excellence for Functional Biomaterials,National University of Ireland Ireland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
Peroxisystem – Understanding peroxisomes as a complete biological system Talk given at a conference Engineering robust stimuli-responsive bio-mimetic polymer compartments 22.02.2016 Weizmann Institute of Israel, Rehovot, Israel Lomora Mihai; Palivan Cornelia;
Peroxisystem – Understanding peroxisomes as a complete biological system Talk given at a conference Artificial peroxisomes: How nanoscience provides therapeutic solutions 22.02.2016 Rehovot, Israel Palivan Cornelia;
Universitäts-Kinderspital beider Basel - Scientific meeting Individual talk Hybrid and biocompatible polymer membranes as tools for mimicking cell compartment 12.01.2016 UKBB , Switzerland Palivan Cornelia; Lomora Mihai;
20Th International Symposium in Microencapsulation Talk given at a conference Protein-polymer assemblies as artificial organelles 01.11.2015 Boston, United States of America Palivan Cornelia;
Technology Apéro NCCR-MSE Poster Nanocompartments with selective permeability by insertion of biopores 29.10.2015 EPFL, Lausanne, Switzerland Lomora Mihai; Palivan Cornelia;
BIT International Conference Talk given at a conference Active nanosystems in therapeutics: Synthetic membranes decorated with biomolecules 22.10.2015 Beijing, China Palivan Cornelia;
SCS Fall Meeting Poster Synthetic biocompartments with selective membrane permeability towards protons and monovalent cations 04.09.2015 EPFL Lausanne, Switzerland Palivan Cornelia; Lomora Mihai;
SCS Fall Meeting Poster Transport of calcium ions through thick biomimetic polymer membranes 04.09.2015 EPFL Lausanne, Switzerland Lomora Mihai; Palivan Cornelia;
International Union of Pure and Applied Chemistry 2015 (IUPAC-2015) Poster Selective ion permeability of biomimetic membranes induced by insertion of biopores 09.08.2015 BEXCO, Busan, Korean Republic (South Korea) Lomora Mihai; Palivan Cornelia;
Invited lecture at the University of Helsinki Talk given at a conference Biomolecules combined with synthetic membranes: Active systems in diagnostics and therapeutics 09.07.2015 Helsinki, Finland Palivan Cornelia;
Invited lecture at the University of Helsinki Individual talk Biomolecules combined with synthetic membranes: Active systems in diagnostics and therapeutics 08.07.2015 Helsinki, Finland Palivan Cornelia;
FBPS ’15 - 11th International Symposium on Frontiers in Biomedical Polymers Talk given at a conference Biomimetic Nano-Compartments with Ion-Selective Membrane Permeability 08.07.2015 Riva del Garda, Italy Palivan Cornelia; Lomora Mihai; Meier Wolfgang;
5Th International colloids Conference Talk given at a conference Synthetic membranes combined with biomolecules: Active systems in diagnostics and therapeutics 19.06.2015 Amsterdam, Netherlands Palivan Cornelia;
Swiss Soft Days 14 Poster Design of an K+ and Na+-responsive nanoreactor 06.06.2015 Basel, Switzerland Lomora Mihai; Palivan Cornelia;
SMART2015, 7Th ECCOMAS tematic Conference on Smart Structures and Materials Talk given at a conference Smart functional nanosystems: Polymer membranes decorated with proteins 04.06.2015 Ponta Delgada Azore, Portugal Palivan Cornelia;
NCCR Lunch Meeting Presentations Talk given at a conference Stimuli-responsive nanocompartments with preserved architecture 20.05.2015 BSSE, Basel, Switzerland Lomora Mihai; Palivan Cornelia; Meier Wolfgang;
Swiss Soft Days 16 Poster Stable and ion selective polymer nanocompartments 04.05.2015 PSI, Villigen, Switzerland Lomora Mihai; Palivan Cornelia;
NCCR Molecular Systems Engineering - 1st Fellow Retreat Poster Stable ions sensitive/stimuli-responsive polymersomes 15.04.2015 Riederalp, Switzerland Lomora Mihai; Palivan Cornelia;
Advanced Functional Polymers for Medicine Talk given at a conference Protein decorated polymer membranes: Novel systems in diagnostics and therapeutics 23.03.2015 Galway, Ireland Palivan Cornelia; Lomora Mihai;
Advanced Functional Polymers for Medicine Talk given at a conference Protein decorated polymer membranes: Novel systems in diagnostics and therapeutics 23.03.2015 Galway, Ireland Lomora Mihai; Palivan Cornelia;
NCCR Technology Apero Poster Artificial organelles by cascade reactions inside polymer compartments 05.12.2014 Basel, Switzerland Palivan Cornelia; Lomora Mihai;
ZHAW Lecture Talk given at a conference Protein containing 3D synthetic assemblies for medical applications 02.11.2014 Wedenswil, Switzerland Palivan Cornelia;
MRS, E-MRS and IUPAC International Conference N.I.C.E.2014 Talk given at a conference Outward and inward passive proton transport through bio-mimetic membranes 15.10.2014 Nice, France Lomora Mihai; Meier Wolfgang; Palivan Cornelia;
Swiss Soft Days 15 Poster Polymer membranes with preserved architecture sensitive for protons, monovalent and divalent cations 02.10.2014 EPF Lausanne, Switzerland Palivan Cornelia; Lomora Mihai;
Nanosmat-9 Poster Towards an ion sensitive nanoreactor 08.09.2014 Trinity College Dublin, Dublin, Ireland Palivan Cornelia; Lomora Mihai;
Nanosmat-9 Talk given at a conference Controlled Reactions in Confined Nanocompartments: At the Threshold of Extraordinary Advances in Nanomedicine 08.09.2014 Trinity College Dublin, Dublin, Ireland Lomora Mihai; Palivan Cornelia;
5th NRP 62 Technology Apéro Poster Outward and inward passive proton transport through bio-mimetic membranes 16.05.2014 Basel, Switzerland Lomora Mihai; Palivan Cornelia;
“Nanoscience In the Snow” Winter School of the Swiss Nanoscience Institute (SNI) Poster pH-responsive nanoreactors by insertion of peptide biopores in polymer membranes 29.01.2014 Kandersteg, Switzerland Lomora Mihai; Palivan Cornelia;
Swiss Soft Days 12 Poster Ion channels as gates for stimuli-responsive nanoreactors 14.10.2013 Bern, Switzerland Palivan Cornelia; Lomora Mihai;
SCS Fall Meeting 2013 Poster Specific artificial organelles detoxify superoxide radicals in cells 04.09.2013 EPF Lausanne, Switzerland Palivan Cornelia; Tanner Pascal;
Supporter in BIOMOD project Individual talk Training on polymersome preparation 07.05.2013 Basel, Switzerland Tanner Pascal; Palivan Cornelia; Meier Wolfgang; Lomora Mihai;
Nanoevent NextNanoStar Talk given at a conference Engineering artificial cell organelles: components and strategy 21.03.2013 Basel, Switzerland Tanner Pascal; Palivan Cornelia;
Swiss Soft Days 8 Poster Specific His6-tag attachement to metal-functionalized polymersomes relies on molecular recognition 01.06.2012 Geneve, Switzerland Tanner Pascal; Palivan Cornelia;


Awards

Title Year
2016 Travel Fellowship by The David Lopatie Conference Centre 2016
M. Lomora, et al. Selective ion-permeable membranes by insertion of biopores into polymersomes, Phys. Chem. Chem. Phys., 2015,17, 15538-15546; - article promoted free of charge to Open Access, via RSC Gold for Gold initiative. 2016
Chemistry Travel Award by SCNAT, SCS and SSFEC, CHIMIA 2015, 69, No. 7/8, doi:10.2533/chimia.2015.478. 2015

Associated projects

Number Title Start Funding scheme
165859 DESIGN OF NANOREACTORS AND ARTIFICIAL ORGANELLES BASED ON POLYMER SUPRAMOLECULAR ASSEMBLIES 01.05.2016 Project funding
156367 Self-defending polymer surfaces: A new strategy to protect medical devices against infection 01.08.2015 China
124406 ANTIOXIDANT NANOREACTORS BASED ON CO-ENCAPSULATION OF ENZYMES IN POLYMERIC VESICLES 01.06.2009 Project funding
139133 Structure, dynamics and interactions of paramagnetic centers characterised by Electrn Paramagnetic Resonance 01.12.2011 R'EQUIP
115956 Structure-activity relationships in Cu/Zn superoxide dismutase (SOD) and SOD-mimics before and after encapsulation in polymeric nanocontainers 01.04.2007 Project funding

Abstract

SUMMARYEfforts to understand the expression of nature’s intelligence and to mimic its structures and functions in the design of new materials and active assemblies have yielded powerful methods in use today in various domains that include chemistry, electronics, materials science and medicine. Of particular interest are polymer supramolecular structures in the form of particles, micelles, vesicles, and films that provide a variety of architectures to allow the insertion/encapsulation/attachment of biomolecules. In this project, we plan to combine physical chemistry, polymer science and enzymatic reactions in order to obtain a better understanding of the structural conformation of biomolecules - polymer supramolecular assemblies and to develop efficient antioxidant nanoreactors and processors. The necessity to develop an antioxidant platform on the nanoscale originates with the increasing evidence of the toxicity of reactive free radicals species generated by oxidative stress as involved in several severe pathological conditions, such as cancer, degenerative diseases, HIV, or viral infections, and recently associated with inorganic nanoparticles or quantum dots.The first objective involves the development of our antioxidant processor concept by designing a multifunctional processor on the nanoscale based on the co-encapsulation of three different enzymes types, allowing for the simultaneous detoxification of oxygen and nitrogen reactive species (O2-, H2O2, and ONNO-). In this regard we will first optimise the antioxidant processor that we previously introduced to detoxify reactive oxygen species, by encapsulating two enzymes that act in tandem inside the cavities of polymer vesicles. Another task will be to study the encapsulation of an enzyme in polymer vesicles to generate a nanoreactor to combat reactive nitrogen species. Together with the optimised two-enzyme processor it will serve to provide the input conditions for the design of a three-enzyme-containing processor. The design of a three-enzyme-containing processor will considerably increase the complexity in terms of processor generation, and reactions taking place inside the cavity of vesicles. As a backup project, we will replace the combination of two enzymes with a dual-enzyme mimic and then co-encapsulate it, together with the third enzyme, inside the polymer vesicles.To avoid tremendous synthetic and preparative effort in the combination of biomolecules and synthetic polymers, an essential approach is to characterise the interactions and structural conformations when controlled variations in the properties of the biomolecule/polymer system are induced by chemical conjugation, or attachment of fluorophores, and spin labels. The second objective of the project is to study in detail the combination of the polymer membrane with biological molecules by: i. insertion of biopores for permeabilization of the membrane, and ii. generation of membranes providing metal centres at their surface for protein binding via molecular recognition interactions. This part of the project represents a fundamental approach to the study of biomolecules that interact with polymer supramolecular assemblies, with respect to the insertion/binding and the related structural changes that occur to accommodate a synthetic material with biomolecules. This will allow us to predict how to choose the copolymer membrane to be combined in a rational design with biomolecules to further develop the platform of nanoreactors and processors in terms of efficacy and targeting approaches. Our overall aim is thus both to improve the basic molecular understanding of the structure and interaction role in combining biomolecules with polymer assemblies, and to design new nanoreactors/processors for further biological and technological applications.
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