Krähenbühl Barbara, Wider Gerhard (2012), Automated projection spectroscopy (APSY) for the assignment of NMR resonances of biological macromolecules., in Chimia
, 66(10), 770-4.
Schubert Mario, Bleuler-Martinez Silvia, Butschi Alex, Wälti Martin A, Egloff Pascal, Stutz Katrin, Yan Shi, Collot Mayeul, Mallet Jean-Maurice, Wilson Iain B H, Hengartner Michael O, Aebi Markus, Allain Frédéric H-T, Künzler Markus (2012), Plasticity of the β-trefoil protein fold in the recognition and control of invertebrate predators and parasites by a fungal defence system., in PLoS pathogens
, 8(5), 1002706-1002706.
Barraud Pierre, Heale Bret S E, O'Connell Mary A, Allain Frédéric H-T (2012), Solution structure of the N-terminal dsRBD of Drosophila ADAR and interaction studies with RNA., in Biochimie
, 94(7), 1499-509.
Campagne S. Damberger F.F. Kaczmarczyk A. Francez-Charlot A. Allain F.H. Vorholt J.A. (2012), Structural basis for sigma factor mimicry in the general stress response of Alphaproteobacteria, in Proc. Natl. Acad. Sci. USA
, 109(21), 1405.
Hiller S. and Wider G. (2012), Automated Projection Spectroscopy and Its Applications, in Curr. Chem.
, 316, 21.
Daubner Gerrit M, Cléry Antoine, Jayne Sandrine, Stevenin James, Allain Frédéric H-T (2012), A syn-anti conformational difference allows SRSF2 to recognize guanines and cytosines equally well., in The EMBO journal
, 31(1), 162-74.
Eichmann C Preissler S Riek R Deuerling E. (2011), Cotranslational structure acquisition of nascent polypeptides monitored by NMR spectroscopy, in Proc Natl Acad Sci U S A
, 107, 9111.
Loughlin F.E. Gebert L.F. Towbin H. Brunschweiger A. Hall J. Allain F.H. (2011), Structural basis of pre-let-7 miRNA recognition by the zinc knuckles of pluripotency factor Lin28, in Nat. Struct. Mol. Biol.
, 19(1), 84.
In the mid 1970's the Institute of Molecular Biology and Biophysics installed a high field NMR laboratory on the initiative of Prof. K. Wüthrich. Due to continuous financial support by private, industrial and public funding the NMR lab could be maintained at the edge of technical developments and the number of hosted NMR spectrometers was gradually increased from one 360 MHz instrument to currently six instruments in the range of 400 to 900 MHz. Since 1995 Prof. Gerhard Wider is the head of the NMR facility and it is in this capacity that he functions as responsible applicant for this application.In this funding application we request new electronics for the 900 MHz NMR spectrometer which was purchased in 2000 and installed in early 2002. The term "electronics" includes all hardware required to operate the instrument except the magnet and the CryoProbe (CryoProbe: trademark of the company Bruker). The superconducting magnets used for NMR spectrometers are not subject to regular wear and tear and can often be operated for 20 and more years. Our 900 MHz magnet is fully functional and fulfills the same specifications as at the time of installation. The CryoProbe is used to detect the NMR signal and in our case was purchased only 2 years ago; this CryoProbe can normally be operated at least another 5 years. All other parts of the 900 MHz spectrometer electronics are in operation since the time of installation. The costs for the new electronics amount to about 10% of the total investment price for a 900 MHz NMR spectrometer that is configured as the existing machine. In the last ten years there were major advances in NMR technology. Modern NMR electronics is more digitized and more miniaturized with the concomitant advantages, e.g. in stability, linearity, dynamic range, reliability and purity of radio-frequencies. One consequence is that the existing CryoProbe will produce a higher sensitivity with the new electronics than on the current system. The technical developments have reached a stage where we can no longer install the newest software on our system. Thus, we are cut-off from all developments even if our system would in principle allow performing some of the new software features. Our NMR spectrometers are operated 24h a day and seven days a week. Thus, at the time of a possible replacement the 900 MHz electronics will have been continuously operated for more than ten years. The signal detected by NMR instruments is very weak and requires the whole instrument to be in top condition for optimal results. So far, obvious failures and malfunctioning of the 900 MHz electronics have increased only slightly, however, this situation can deteriorate any time. One measurement may take up to three days and one little glitch in the electronics can severely impair or even destroy the whole measurement. Since the life time of the measured samples may be very limited, one failed experiment can produce considerable loss in time and may require substantial additional financial resources. Last year the NMR lab of the Institute of Molecular Biology and Biophysics has become a tech-nology platform of the Biology Department of the ETH which increased the number of potential users substantially. Currently, on the 900 MHz spectrometers the co-applicants Prof. Frederic Allain and Prof. Roland Riek (D-CHAB) are still the main users. The 900 MHz NMR spectrometer at the ETH is the instrument with the highest magnetic field and the highest sensitivity in Switzerland and is also used by researchers from outside the ETH. In summary, the purchase of new electronics for the 900 MHz spectrometer at the ETH is absolutely essential for the continuation of a competitive research at the forefront of NMR spectroscopy applied to structural biology.