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Application of Synchrotron-based Fourier Transform Infrared Microspectroscopy

English title Application of Synchrotron-based Fourier Transform Infrared Microspectroscopy
Applicant Forro Laszlo
Number 138306
Funding scheme Project funding
Research institution Laboratoire de nanostructures et nouveaux matériaux électroniques EPFL - SB - IPMC - LNNME
Institution of higher education EPF Lausanne - EPFL
Main discipline Condensed Matter Physics
Start/End 01.02.2012 - 28.02.2015
Approved amount 190'110.00
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Keywords (4)

scanning infrared spectro-microscopy; toxicity effect of nanostructures; protein conformational changes; neurodegenerative diseases

Lay Summary (English)

Lead
Lay summary

Infrared spectroscopy is a powerful technique in the study of condensed matter since the energy of electronic excitations and lattice vibrations fall in its range. The lattice vibrations are especially useful in soft condensed matter where the change is phonon modes signal conformational changes, oxidative stress or other bio-chemical processes in the living matter. Since the development of very bright infrared sources based on synchrotron radiation, the scanning infrared spectro-microscopy (SIRMS) enables the creation of a vibrational map of tissues, cells with a spatial resolution of few microns. This allows the localization of regions where structural/biochemical changes have happened. These maps carry quantitative information of the sample in contrast to simple fluorescence imaging.

The subject of this proposal inscribes into an interdisciplinary research. We plan to apply SIRMS technique to investigate topics of major interest in living matter: i) neurodegenerative diseases (Huntington and multiple sclerosis) where the formation of plaques, and the correlations of demyelization in white and gray matter will be followed on animal models; and ii) the toxicity effect of nanostructures, where the biochemical precursors and consequences of cell death will be addressed.

These measurements will be performed at the newly created SIRMS facility of the Swiss Light Source (SLS) at Villigen.

 

Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Name Institute

Collaboration

Group / person Country
Types of collaboration
Hooke Laboratories, Lauwrence, Ma United States of America (North America)
- in-depth/constructive exchanges on approaches, methods or results
- Research Infrastructure
Uni Fribourg, Dept of Medicine Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Research Infrastructure
- Exchange of personnel
Swiss Light Source Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Research Infrastructure
EPFL, School of Life Sciences Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
YUCOMAT Talk given at a conference Toxic effect of nanostructures 03.09.2013 Herceg Novi, Montenegro Forro Laszlo;
YUCOMAT Talk given at a conference Toxic effect of nanostructures 03.09.2013 Herceg Novi, Montenegro Forro Laszlo;
Adriatic School on nanoscience Talk given at a conference TiO2 nanowires_from basic studies to applications 02.09.2012 Dubrovnik, Croatia Forro Laszlo;


Associated projects

Number Title Start Funding scheme
125299 Toxicity study of nanostructures 01.04.2009 Project funding
156981 Application of Synchrotron-based Fourier Transform Infrared Microspectroscopy 01.03.2015 Project funding

Abstract

Infrared spectroscopy is a powerful technique in the study of condensed matter since the energy of electronic excitations and lattice vibrations fall in its range. The lattice vibrations are especially useful in soft condensed matter where the change is phonon modes signal conformational changes, oxidative stress or other bio-chemical processes in the living matter. Since the development of very bright infrared sources based on synchrotron radiation, the scanning infrared spectro-microscopy (SIRMS) enables the creation of a vibrational map of tissues, cells with a spatial resolution of few microns. This allows the localization of regions where structural/biochemical changes have happened. These maps carry quantitative information of the sample in contrast to simple fluorescence imaging.The subject of this proposal inscribes into an interdisciplinary research. We plan to apply SIRMS technique to investigate topics of major interest in living matter: i) neurodegenerative diseases (Huntington and multiple sclerosis) where the formation of plaques, and the correlations of demyelization in white and gray matter will be followed on animal models; and ii) the toxicity effect of nanostructures, where the biochemical precursors and consequences of cell death will be addressed. These measurements will be performed at the newly created SIRMS facility of the Swiss Light Source (SLS) at Villigen.
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