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ABC transporters involved in signalling events

English title ABC transporters involved in signalling events
Applicant Martinoia Enrico
Number 135747
Funding scheme Project funding (Div. I-III)
Research institution Institut für Pflanzen- und Mikrobiologie Universität Zürich
Institution of higher education University of Zurich - ZH
Main discipline Biochemistry
Start/End 01.04.2011 - 31.03.2014
Approved amount 537'000.00
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Keywords (1)

ABC transporter,guard cell,mycorrhiza,branching

Lay Summary (English)

Lead
Lay summary

ATP-binding cassette (ABC)proteins are ubiquitously found in prokaryotes and eukaryotes. Theycatalyze the primary active transport of a multitude of substrates, such asglutathionated compounds, alkaloids, terpenoids, lipids, heavy metals andpeptides. Recent progress on plant ABCtransporters demonstrates that they transport substrates, which have not beendescribed in other organisms such as auxins and abscisic acid (ABA), as well asthe main storage compound for phosphate, , phytate), which also acts as asignalling compound6phosphate (InsPkisinositol hexa.

In the present project wewill address two questions related to ABC transporters with the aim toelucidate the role of these transporters in plant hormone transport and signaling

transport. 6 compartmentationaffects hormone levels and how interacting partners modulate InsP6 transport affects auxin and/or ethylene levels.Based on these results we will investigate how InsP6transport affectsguard cell functioning. We identified interacting partners and got first indicationsthat impaired InsP6 transporter. Sofar it is unknown, how altered vacuolar InsP6 andauxin. Recently we showed that AtABCC5 is an InsP2+(A) Loss-of-functionmutants for AtABCC5 are impaired in their response to ABA, Ca

(B) In the second projectwe are interested to identify and characterize a strigolactone transporter. Strigolactonesare involved in two diverse processes, mycorrhization and shoot branching. Wefound that the Petunia Phpdr1 mutants are impaired in mycorrhization andexhibit increased shoot branching, indicating that this ABC transporter couldbe involved in  export of strigolactone formstrigolactone-producing cells. In the frame of the present project we envisageto further characterize the transport activity, the localization and thefunction of PhPDR1 in mycorrhization and shoot branching.

Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Publications

Publication
An ATP-binding cassette protein from grape berry (VvABCC1) transports glucosylated anthocyanins.
Francisco R, Regalado A, Agegeorges A, Burla B, Bassin B, Eisenach C, zarouk O, Chaves MM, Martinoia E, Nagy R (2013), An ATP-binding cassette protein from grape berry (VvABCC1) transports glucosylated anthocyanins., in The Plant Cell, 25, 1840-1854.
AtALMT9 is a malate-activated vacuolar chloride channel required for stomatal opening in Arabidopsis
De Angeli Alexis, Zhang Jingbo, Meyer Stefan, Martinoia Enrico (2013), AtALMT9 is a malate-activated vacuolar chloride channel required for stomatal opening in Arabidopsis, in Nature Communication, 4, 1804.
Identification of a probable pore forming domain in the multimeric vacuolar anion channel AtALMT9
Zang J, Bätz U, Krügel U, Martinoia E, De Angeli A (2013), Identification of a probable pore forming domain in the multimeric vacuolar anion channel AtALMT9, in Plant Physiology, 163, 830-843.
Vacuolar Transport of Abscisic Acid Glucosyl Ester is Mediated by ATP-Binding Cassette and Proton-Antiport Mechanisms in Arabidopsis thaliana.
Burla B, Pfrunder S, Nagy R, Francisco R, Lee Y, Martinoia E (2013), Vacuolar Transport of Abscisic Acid Glucosyl Ester is Mediated by ATP-Binding Cassette and Proton-Antiport Mechanisms in Arabidopsis thaliana., in Plant Physiology, 163, 1446-1458.
A petunia ABC protein controls strigolactone-dependent symbiotic signalling and branching.
Kretzschmar Tobias, Kohlen Wouter, Sasse Joelle, Borghi Lorenzo, Schlegel Markus, Bachelier Julien B, Reinhardt Didier, Bours Ralph, Bouwmeester Harro J, Martinoia Enrico (2012), A petunia ABC protein controls strigolactone-dependent symbiotic signalling and branching., in Nature, 483(7389), 341-4.
AtABCG29 is a monolignol transporter involved in lignin biosynthesis.
Alejandr Santiago, Lee Yuree, Toghe Takayuki, Sudre Damien, Osorio Sonia, Park Jiyoung, Bovet Lucien, Lee Youngsook, Geldner Niko, Fernie Alisdair, Martinoia Enrico (2012), AtABCG29 is a monolignol transporter involved in lignin biosynthesis., in Current Biology, 22(13), 1207-1212.
The phytochelatin transporters AtABCC1 and AtABCC2 mediate tolerance to cadmium and mercury.
Park Jiyoung, Song Won-Yong, Ko Donghwi, Eom Yujin, Hansen Thomas H, Schiller Michaela, Lee Tai Gyu, Martinoia Enrico, Lee Youngsook (2012), The phytochelatin transporters AtABCC1 and AtABCC2 mediate tolerance to cadmium and mercury., in The Plant journal : for cell and molecular biology, 69(2), 278-88.
Vacuolar Transporters in Their Physiological Context.
Martinoia Enrico, Meyer Stefan, De Angeli Alexis, Nagy Réka (2012), Vacuolar Transporters in Their Physiological Context., in Annual Review of Plant Biology, 63, 183-213.
Malate transport by the vacuolar AtALMT6 channel in guard cells is subject to multiple regulation
Meyer S, Scholz-Starke J, De Angeli A, Kovermann P, Burla B, Gambale F, Martinoia E (2011), Malate transport by the vacuolar AtALMT6 channel in guard cells is subject to multiple regulation, in PLANT JOURNAL, 67(2), 247-257.
Plant ABC Transporters.
Kang Joohyun, Park Jiyoung, Choi Hyunju, Burla Bo, Kretzschmar Tobias, Lee Youngsook, Martinoia Enrico (2011), Plant ABC Transporters., in The Arabidopsis book / American Society of Plant Biologists, 9, 0153-0153.
Arabidopsis ABCG14 is essential for root-to-shoot translocation of cytokinin
Ko D, Kang J, Kiba T, Park J, Kojima M, Do J, Kim K-Y, Kwon M, Endler A, Song W-Y, Martinoia E, Sakakibara H, Lee Y, Arabidopsis ABCG14 is essential for root-to-shoot translocation of cytokinin, in Proc. Natl. Acad, Sci. USA.

Collaboration

Group / person Country
Types of collaboration
Prof. Harro Bouwmeester Netherlands (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Dr. Didier Reinhardt Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
Getting a cell smart: green factories forthe next generation of pharmaceuticals Talk given at a conference Different roles of the Petunia PDR1 transporter 23.01.2014 Leiden, Netherlands Martinoia Enrico;
International Symposium on Plant Apoplastic Diffusion Barriers: Biosynthesis and Functions Talk given at a conference Monolignol Transport 05.09.2013 Lausanne, Switzerland Martinoia Enrico;
PhD School on Plant nutrition Talk given at a conference The role of the vacuole in plant nutrition 24.06.2013 Gargnano, Italy Martinoia Enrico;
2013 International Symposium on Agricultural Biotechnology , Taipei, Taiwan Talk given at a conference AtABCG36, an ABC transporter involved in biotic and abiotic stress responses 24.05.2013 Taipei, Taiwan Martinoia Enrico;
XVI International Workshop Plant Membrane Biology Talk given at a conference A Petunia ABC transporter controls a multitude of strigolactone-dependent functions 26.03.2013 Kurashiki, Japan, Japan Martinoia Enrico; Borghi Lorenzo;
26. Tagung Molekularbiologie der Pflanzen Talk given at a conference The role of ABC transporters in abscisic acid and strigolactone transport 26.02.2013 Dabringhausen, Germany Martinoia Enrico;
Gordon Conference on Carotenoids Talk given at a conference The role of ABC transporters in strigolactone transport 06.01.2013 Ventura, Ca, United States of America Martinoia Enrico; Borghi Lorenzo;
10th International Congress on Plant Molecular Biology Talk given at a conference The ABC transporter PDR1 regulates strigolactone-dependent shoot branching and mycorrhization 21.10.2012 Jeju, Korean Republic (South Korea) Borghi Lorenzo; Martinoia Enrico;
International Winter Conference in Plant Biology Talk given at a conference Identification of a Strigolactone transporter 12.01.2012 Pohang, Korea, Korean Republic (South Korea) Martinoia Enrico;
Deutsche Botanikertagung Talk given at a conference Aluminium-activated malate transporters (ALMTs): Channels with multiple functions and complex regulation 18.09.2011 Berlin, Germany Martinoia Enrico;
Metals in Biology, Intl. one-day workshop Talk given at a conference Intra-and extracellular detoxification 06.09.2011 Göteborg, Sweden Martinoia Enrico;
COBTE, Florence, Italy; 3.7.2011-8.7.2011 Talk given at a conference Heavy metal transporters in plant roots: Detoxification by excretion and internalization 03.07.2011 Florenz, Italien, Italy Martinoia Enrico;


Communication with the public

Communication Title Media Place Year
Talks/events/exhibitions Fluch und Segen der Schwermetalle German-speaking Switzerland 2013
Talks/events/exhibitions Ist Wissenschaft alles? German-speaking Switzerland 2013
Talks/events/exhibitions Schwermetalle: Fluch und Segen German-speaking Switzerland 2012

Awards

Title Year
Cozzarelli Prize for the best paper in Applied Biology, Agriculture and Environmental Sciences, PNAS 2011

Patents

Title Date Number Inventor Owner
Recombinant vectors modifying the expression levels of monolignol transporter genes, and transgenic 25.05.2012 10-2012-0056015/P2012112-01-KR

Associated projects

Number Title Start Funding scheme
117790 Regulation mechanisms involved in plant transport processes 01.10.2007 Project funding (Div. I-III)
145020 Plant metabolite analysis by liquid chromatography-mass spectrometry 01.10.2013 R'EQUIP
152831 Investigation of phytohormone transport and the fate of hypodermal passage cells, a cell type specialized to release strigolactones 01.05.2014 Project funding (Div. I-III)

Abstract

1.1. BackgroundATP-binding cassette (ABC) proteins are ubiquitously found in prokaryotes and eukaryotes. They catalyze the primary active transport of a multitude of substrates, such as glutathionated compounds, alkaloids, terpenoids, lipids, heavy metals and peptides. Genome-wide inventories of ABC transporters in Arabidopsis, rice and poplar led to the identification of more than 100 loci encoding for either half-size or full-size transporters in each, making them nearly twice as numerous in plants as reported for sequenced animal species. Recent progress on plant ABC transporters demonstrates that they transport substrates which have not been described in other organisms. It has been shown that they transport several plant hormones such as auxins and abscisic acid (ABA), as well as the main storage compound for phosphate, inositol hexakisphosphate (InsP6, phytate), which also acts as a signalling compound.1.2. Questions we will address in this project(A) Loss-of-function mutants for AtABCC5 are impaired in their response to ABA, Ca2+ and auxin. Recently we showed that AtABCC5 is an InsP6 transporter. So far it is unknown, how altered vacuolar InsP6 transport affects guard cell functioning. Through the comparative analysis with HLS1, an AtABCC5 interacting partner identified in a yeast two-hybrid screen, we discovered that auxin and/or ethylene levels are altered in atabcc5. Based on these results we will investigate how InsP6 compartmentation affects hormone levels, how interacting partners modulate InsP6 transport and whether the response to pathogens is similarly impaired in atabcc5 as in mutants not producing Insp6. (B) In a project initiated within the framework of the NCCR we identified PhPDR1, a putative strigolactone transporter in Petunia hybrida. Strigolactones are involved in two diverse processes, mycorrhization and shoot branching. We found that Phpdr1 mutants are impaired in mycorrhization and exhibit increased shoot branching, which is at least in part due to a dysfunctional export form strigolactone-producing cells. In the frame of the present project we envisage to further characterize the transport activity and localization of PhPDR1 as well as the interaction between strigolactone and auxin transport. Belowground PhPDR1 is mainly expressed in hypodermal passage cells, the cortical entry point for mycorrhizal fungi. Using a PhPDR1 promoter-GFP construct to specifically isolate this cell type, combined with large scale quantitative transcriptomics, we plan to elucidate the special characteristics/ of hypodermal passage cells.1.3. Expected value of the projectThe aim of this project is to analyze the transport of novel plant hormones and signalling compounds via ABC proteins and the physiological effects that impaired transport of these compounds can have. The results will broaden our understanding of regulatory processes in plants, particularly the interaction between different plant hormones. Furthermore, the investigation of hypodermal passage cells, which so far could not be addressed due to the lack of molecular markers, will allow elucidating the function of this cell type.
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