Francisco R, Regalado A, Agegeorges A, Burla B, Bassin B, Eisenach C, zarouk O, Chaves MM, Martinoia E, Nagy R (2013), An ATP-binding cassette protein from grape berry (VvABCC1) transports glucosylated anthocyanins., in The Plant Cell
, 25, 1840-1854.
De Angeli Alexis, Zhang Jingbo, Meyer Stefan, Martinoia Enrico (2013), AtALMT9 is a malate-activated vacuolar chloride channel required for stomatal opening in Arabidopsis, in Nature Communication
, 4, 1804.
Zang J, Bätz U, Krügel U, Martinoia E, De Angeli A (2013), Identification of a probable pore forming domain in the multimeric vacuolar anion channel AtALMT9, in Plant Physiology
, 163, 830-843.
Burla B, Pfrunder S, Nagy R, Francisco R, Lee Y, Martinoia E (2013), Vacuolar Transport of Abscisic Acid Glucosyl Ester is Mediated by ATP-Binding Cassette and Proton-Antiport Mechanisms in Arabidopsis thaliana., in Plant Physiology
, 163, 1446-1458.
Kretzschmar Tobias, Kohlen Wouter, Sasse Joelle, Borghi Lorenzo, Schlegel Markus, Bachelier Julien B, Reinhardt Didier, Bours Ralph, Bouwmeester Harro J, Martinoia Enrico (2012), A petunia ABC protein controls strigolactone-dependent symbiotic signalling and branching., in Nature
, 483(7389), 341-4.
Alejandr Santiago, Lee Yuree, Toghe Takayuki, Sudre Damien, Osorio Sonia, Park Jiyoung, Bovet Lucien, Lee Youngsook, Geldner Niko, Fernie Alisdair, Martinoia Enrico (2012), AtABCG29 is a monolignol transporter involved in lignin biosynthesis., in Current Biology
, 22(13), 1207-1212.
Park Jiyoung, Song Won-Yong, Ko Donghwi, Eom Yujin, Hansen Thomas H, Schiller Michaela, Lee Tai Gyu, Martinoia Enrico, Lee Youngsook (2012), The phytochelatin transporters AtABCC1 and AtABCC2 mediate tolerance to cadmium and mercury., in The Plant journal : for cell and molecular biology
, 69(2), 278-88.
Martinoia Enrico, Meyer Stefan, De Angeli Alexis, Nagy Réka (2012), Vacuolar Transporters in Their Physiological Context., in Annual Review of Plant Biology
, 63, 183-213.
Meyer S, Scholz-Starke J, De Angeli A, Kovermann P, Burla B, Gambale F, Martinoia E (2011), Malate transport by the vacuolar AtALMT6 channel in guard cells is subject to multiple regulation, in PLANT JOURNAL
, 67(2), 247-257.
Kang Joohyun, Park Jiyoung, Choi Hyunju, Burla Bo, Kretzschmar Tobias, Lee Youngsook, Martinoia Enrico (2011), Plant ABC Transporters., in The Arabidopsis book / American Society of Plant Biologists
, 9, 0153-0153.
Ko D, Kang J, Kiba T, Park J, Kojima M, Do J, Kim K-Y, Kwon M, Endler A, Song W-Y, Martinoia E, Sakakibara H, Lee Y, Arabidopsis ABCG14 is essential for root-to-shoot translocation of cytokinin, in Proc. Natl. Acad, Sci. USA
1.1. BackgroundATP-binding cassette (ABC) proteins are ubiquitously found in prokaryotes and eukaryotes. They catalyze the primary active transport of a multitude of substrates, such as glutathionated compounds, alkaloids, terpenoids, lipids, heavy metals and peptides. Genome-wide inventories of ABC transporters in Arabidopsis, rice and poplar led to the identification of more than 100 loci encoding for either half-size or full-size transporters in each, making them nearly twice as numerous in plants as reported for sequenced animal species. Recent progress on plant ABC transporters demonstrates that they transport substrates which have not been described in other organisms. It has been shown that they transport several plant hormones such as auxins and abscisic acid (ABA), as well as the main storage compound for phosphate, inositol hexakisphosphate (InsP6, phytate), which also acts as a signalling compound.1.2. Questions we will address in this project(A) Loss-of-function mutants for AtABCC5 are impaired in their response to ABA, Ca2+ and auxin. Recently we showed that AtABCC5 is an InsP6 transporter. So far it is unknown, how altered vacuolar InsP6 transport affects guard cell functioning. Through the comparative analysis with HLS1, an AtABCC5 interacting partner identified in a yeast two-hybrid screen, we discovered that auxin and/or ethylene levels are altered in atabcc5. Based on these results we will investigate how InsP6 compartmentation affects hormone levels, how interacting partners modulate InsP6 transport and whether the response to pathogens is similarly impaired in atabcc5 as in mutants not producing Insp6. (B) In a project initiated within the framework of the NCCR we identified PhPDR1, a putative strigolactone transporter in Petunia hybrida. Strigolactones are involved in two diverse processes, mycorrhization and shoot branching. We found that Phpdr1 mutants are impaired in mycorrhization and exhibit increased shoot branching, which is at least in part due to a dysfunctional export form strigolactone-producing cells. In the frame of the present project we envisage to further characterize the transport activity and localization of PhPDR1 as well as the interaction between strigolactone and auxin transport. Belowground PhPDR1 is mainly expressed in hypodermal passage cells, the cortical entry point for mycorrhizal fungi. Using a PhPDR1 promoter-GFP construct to specifically isolate this cell type, combined with large scale quantitative transcriptomics, we plan to elucidate the special characteristics/ of hypodermal passage cells.1.3. Expected value of the projectThe aim of this project is to analyze the transport of novel plant hormones and signalling compounds via ABC proteins and the physiological effects that impaired transport of these compounds can have. The results will broaden our understanding of regulatory processes in plants, particularly the interaction between different plant hormones. Furthermore, the investigation of hypodermal passage cells, which so far could not be addressed due to the lack of molecular markers, will allow elucidating the function of this cell type.