Dynamic Nuclear Polarization (DNP); Dissolution DNP in liquids; Slow dynamic processes in proteins; Long-lived spin states in liquids; ‘In situ' DNP in solids; DNP-enhanced surface NMR; Fast correlated dynamics in proteins; Nitrogen-14 Solid-State NMR
Kobayashi Takeshi, Lafon Olivier, Thankamony Aany S Lilly, Slowing Igor I, Kandel Kapil, Carnevale Diego, Vitzthum Veronika, Vezin Hervé, Amoureux Jean-Paul, Bodenhausen Geoffrey, Pruski Marek (2013), Analysis of sensitivity enhancement by dynamic nuclear polarization in solid-state NMR: a case study of functionalized mesoporous materials., in
Physical chemistry chemical physics : PCCP, 15(15), 5553-62.
Lafon O, Thankamony ASL, Kobayashi T, Carnevale D, Vitzthum V, Slowing II, Kandel K, Vezin H, Amoureux J-P, Bodenhausen G, Pruski M (2013), Mesoporous silica nanoparticles loaded with surfactant: Low temperature magic angle spinning 13C and 29Si NMR enhanced by dynamic nuclear polarization, in
Journal of Physical Chemistry C, 117(3), 1375-1382.
Segawa TF, Carnevale D, Bodenhausen G (2013), How to tickle spins with a fourier transform NMR spectrometer, in
ChemPhysChem, 14(2), 369-373.
Bornet A, Melzi R, Perez Linde AJ, Hautle P, Van Den Brandt B, Jannin S, Bodenhausen G (2013), Boosting dissolution dynamic nuclear polarization by cross polarization, in
Journal of Physical Chemistry Letters, 4(1), 111-114.
Bornet A, Milani J, Wang S, Mammoli D, Buratto R, Salvi N, Segawa TF, Vitzthum V, Miéville P, Srichinthalapalli N, Perez-Linde AJ, Carnevale D, Jannin S, Caporini M, Ulzega S, Rey M, Bodenhausen G (2012), Dynamic Nuclear Polarization and other magnetic ideas at EPFL, in
Chimia, 66(10), 734-740.
Jannin S, Bornet A, Melzi R, Bodenhausen G (2012), High field dynamic nuclear polarization at 6.7 T: Carbon-13 polarization above 70% within 20 min, in
Chemical Physics Letters, 549, 99-102.
Vitzthum V, Caporini MA, Ulzega S, Trébosc J, Lafon O, Amoureux J-P, Bodenhausen G (2012), Uniform broadband excitation of crystallites in rotating solids using interleaved sequences of delays alternating with nutation, in
Journal of Magnetic Resonance, 223, 228-236.
Augustyniak Rafal, Ferrage Fabien, Damblon Christian, Bodenhausen Geoffrey, Pelupessy Philippe (2012), Efficient determination of diffusion coefficients by monitoring transport during recovery delays in NMR, in
CHEMICAL COMMUNICATIONS, 48(43), 5307-5309.
Carnevale Diego, Vitzthum Veronika, Lafon Olivier, Trebosc Julien, Amoureux Jean-Paul, Bodenhausen Geoffrey (2012), Broadband excitation in solid-state NMR of paramagnetic samples using Delays Alternating with Nutation for Tailored Excitation ('Para-DANTE'), in
CHEMICAL PHYSICS LETTERS, 553, 68-76.
Carnevale Diego, Segawa Takuya F., Bodenhausen Geoffrey (2012), Polychromatic Decoupling of a Manifold of Homonuclear Scalar Interactions in Solution-State NMR, in
CHEMISTRY-A EUROPEAN JOURNAL, 18(37), 11573-11576.
Bornet Aurelien, Melzi Roberto, Jannin Sami, Bodenhausen Geoffrey (2012), Cross Polarization for Dissolution Dynamic Nuclear Polarization Experiments at Readily Accessible Temperatures 1.2 < T < 4.2 K, in
APPLIED MAGNETIC RESONANCE, 43(1-2), 107-117.
Chinthalapalli Srinivas, Bornet Aurélien, Segawa Takuya F, Sarkar Riddhiman, Jannin Sami, Bodenhausen Geoffrey (2012), Ultrahigh-resolution magnetic resonance in inhomogeneous magnetic fields: two-dimensional long-lived-coherence correlation spectroscopy., in
Physical review letters, 109(4), 047602-047602.
Salvi N, Buratto R, Bornet A, Ulzega S, Rebollo IR, Angelini A, Heinis C, Bodenhausen G (2012), Boosting the Sensitivity of Ligand-Protein Screening by NMR of Long-Lived States, in
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 134(27), 11076-11079.
Calligari Paolo A, Salgado Gilmar F, Pelupessy Philippe, Lopes Philippe, Ouazzani Jamal, Bodenhausen Geoffrey, Abergel Daniel (2012), Insights into internal dynamics of 6-phosphogluconolactonase from Trypanosoma brucei studied by nuclear magnetic resonance and molecular dynamics., in
Proteins, 80(4), 1196-210.
Segawa TF, Baishya B, Bodenhausen G (2012), Transverse Relaxation of Scalar Coupled Protons in Magnetic Resonance of Non-Deuterated Proteins, in
APPLIED MAGNETIC RESONANCE, 42(3), 353-361.
Carnevale D, Bodenhausen G (2012), Composite pulses for efficient excitation of half-integer quadrupolar nuclei in NMR of static and spinning solid samples, in
CHEMICAL PHYSICS LETTERS, 530, 120-125.
Vitzthum Veronika, Miéville Pascal, Carnevale Diego, Caporini Marc A, Gajan David, Copéret Christophe, Lelli Moreno, Zagdoun Alexandre, Rossini Aaron J, Lesage Anne, Emsley Lyndon, Bodenhausen Geoffrey (2012), Dynamic nuclear polarization of quadrupolar nuclei using cross polarization from protons: surface-enhanced aluminium-27 NMR., in
Chemical communications (Cambridge, England), 48(14), 1988-90.
Salvi N, Ulzega S, Ferrage F, Bodenhausen G (2012), Time Scales of Slow Motions in Ubiquitin Explored by Heteronuclear Double Resonance, in
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 134(5), 2481-2484.
Aguilar JA, Nilsson M, Bodenhausen G, Morris GA (2012), Spin echo NMR spectra without J modulation, in
CHEMICAL COMMUNICATIONS, 48(6), 811-813.
Augustyniak R, Balayssac S, Ferrage F, Bodenhausen G, Lequin O (2011), 1H, 13C and 15N resonance assignment of a 114-residue fragment of Engrailed 2 homeoprotein, a partially disordered protein., in
Biomolecular NMR assignments, 5(2), 229-231.
Bodenhausen G (2011), Reflections of pathways: A short perspective on 'Selection of coherence transfer pathways in NMR pulse experiments, in
Journal of Magnetic Resonance, 213(2), 295-297.
Segawa TF, Bornet A, Salvi N, Mieville P, Vitzthum V, Carnevale D, Jannin S, Caporini MA, Ulzega S, Vasos PR, Rey M, Bodenhausen G (2011), Extending Timescales and Narrowing Linewidths in NMR, in
CHIMIA, 65(9), 652-655.
Mieville P, Jannin S, Helm L, Bodenhausen G (2011), NMR of Insensitive Nuclei Enhanced by Dynamic Nuclear Polarization, in
CHIMIA, 65(4), 260-263.
Herbert-Pucheta JE, Colaux H, Bodenhausen G, Tekely P (2011), Disentangling Crystallographic Inequivalence and Crystallographic Forms of L-Arginine by One- and Two-Dimensional Solid-State NMR Spectroscopy, in
JOURNAL OF PHYSICAL CHEMISTRY B, 115(51), 15415-15421.
Jannin S, Bornet A, Colombo S, Bodenhausen G (2011), Low-temperature cross polarization in view of enhancing dissolution Dynamic Nuclear Polarization in NMR, in
CHEMICAL PHYSICS LETTERS, 517(4-6), 234-236.
Mieville P, Vitzthum V, Caporini MA, Jannin S, Gerber-Lemaire S, Bodenhausen G (2011), A spinning thermometer to monitor microwave heating and glass transitions in dynamic nuclear polarization, in
MAGNETIC RESONANCE IN CHEMISTRY, 49(11), 689-692.
Vitzthum V, Borcard F, Jannin S, Morin M, Mieville P, Caporini MA, Sienkiewicz A, Gerber-Lemaire S, Bodenhausen G (2011), Fractional Spin-Labeling of Polymers for Enhancing NMR Sensitivity by Solvent-Free Dynamic Nuclear Polarization, in
CHEMPHYSCHEM, 12(16), 2929-2932.
Bornet A, Ahuja P, Sarkar R, Fernandes L, Hadji S, Lee SY, Haririnia A, Fushman D, Bodenhausen G, Vasos PR (2011), Long-Lived States to Monitor Protein Unfolding by Proton NMR, in
CHEMPHYSCHEM, 12(15), 2729-2734.
Bornet A, Jannin S, Konter JA, Hautle P, van den Brandt B, Bodenhausen G (2011), Ultra High-Resolution NMR: Sustained Induction Decays of Long-Lived Coherences, in
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 133(39), 15644-15649.
Vitzthum V, Caporini MA, Ulzega S, Bodenhausen G (2011), Broadband excitation and indirect detection of nitrogen-14 in rotating solids using Delays Alternating with Nutation (DANTE), in
JOURNAL OF MAGNETIC RESONANCE, 212(1), 234-239.
Baishya B, Segawa TF, Bodenhausen G (2011), Quenching homonuclear couplings in magnetic resonance by trains of non-refocusing pulses, in
JOURNAL OF MAGNETIC RESONANCE, 211(2), 240-242.
Bornet A, Jannin S, Bodenhausen G (2011), Three-field NMR to preserve hyperpolarized proton magnetization as long-lived states in moderate magnetic fields, in
CHEMICAL PHYSICS LETTERS, 512(4-6), 151-154.
Weingarth M, Trebosc J, Amoureux JP, Bodenhausen G, Tekely P (2011), Efficiency at high spinning frequencies of heteronuclear decoupling methods designed to quench rotary resonance, in
SOLID STATE NUCLEAR MAGNETIC RESONANCE, 40(1), 21-26.
Sarkar R, Ahuja P, Vasos PR, Bornet A, Wagnieres O, Bodenhausen G (2011), Long-lived coherences for line-narrowing in high-field NMR, in
PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY, 59(1), 83-90.
Augustyniak R, Ferrage F, Paquin R, Lequin O, Bodenhausen G (2011), Methods to determine slow diffusion coefficients of biomolecules. Applications to Engrailed 2, a partially disordered protein, in
JOURNAL OF BIOMOLECULAR NMR, 50(3), 209-218.
Weingarth Markus, Masuda Yuichi, Takegoshi K, Bodenhausen Geoffrey, Tekely Piotr (2011), Sensitive (13)C- (13)C correlation spectra of amyloid fibrils at very high spinning frequencies and magnetic fields., in
Journal of biomolecular NMR, 50(2), 129-36.
Mieville P, Jannin S, Bodenhausen G (2011), Relaxometry of insensitive nuclei: Optimizing dissolution dynamic nuclear polarization, in
JOURNAL OF MAGNETIC RESONANCE, 210(1), 137-140.
Lelli M, Gajan D, Lesage A, Caporini MA, Vitzthum V, Mieville P, Heroguel F, Rascon F, Roussey A, Thieuleux C, Boualleg M, Veyre L, Bodenhausen G, Coperet C, Emsley L (2011), Fast Characterization of Functionalized Silica Materials by Silicon-29 Surface-Enhanced NMR Spectroscopy Using Dynamic Nuclear Polarization, in
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 133(7), 2104-2107.
Ulzega S, Salvi N, Segawa TF, Ferrage F, Bodenhausen G (2011), Control of Cross Relaxation of Multiple-Quantum Coherences Induced by Fast Chemical Exchange under Heteronuclear Double-Resonance Irradiation, in
CHEMPHYSCHEM, 12(2), 333-341.
Nuclear Magnetic Resonance (NMR) spectroscopy is a formidable tool for the investigation of matter through the measurement of nuclear spin interactions that has virtually infinite applications in numerous disciplines of science. This research plan is based on five subprojects. (A) Long-Lived States (LLS’s) of nuclear spins exhibit lifetimes that can be much longer than the spin-lattice relaxation time T1 (TLLS >> T1) and thus represent extremely interesting probes for slow dynamic processes. We will develop methods to excite and sustain LLS’s in proteins and access dynamics such as folding/unfolding processes in complex biomolecules. We also plan to search for LLS’s in water molecules and possibly exploit them to study slow processes such as hindered diffusion and metabolic chemical reactions. (B) We have recently developed new Heteronuclear Double Resonance (HDR) methods that give us access to fast correlated processes in biomolecules. These methods will offer a unique insight into dynamic modes that extend over contiguous regions of a protein structure. They could also be extended to nucleic acids (e.g., RNA). (C) Dynamic Nuclear Polarization (DNP) has the potential of enhancing NMR signals by several orders of magnitude by coupling nuclear spins to the large reservoir of electrons. A solid-state DNP - NMR system (in-situ DNP) has been recently installed at EPFL and will be used to investigate the largely unexplored field of surface chemistry. Surface science is a very active research area for technological innovations that can boast a number of Nobel Prizes in chemistry and significant financial investments. (D) In liquids, we have successfully applied Dissolution-DNP to various nuclei and developed novel challenging methods. We have shown that hyperpolarized magnetization can be stored in long-lived nuclear spin states (DNP - LLS). This method will be further developed and exploited to study slow processes, such as diffusion phenomena and metabolic reactions. We will also carry out further work on scavenging of free radicals, relaxometry, proton DNP and DNP of macromolecules. (E) Our lab, a worldwide leader in solid-state 14N NMR spectroscopy, will be soon (2011) equipped with a probe for fast (up to 70 kHz) magic angle spinning (MAS). This will boost our efforts directed to enhance the efficiency of our solid-state methods, with a special focus on the investigation of fast local dynamics.