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Perception and signalling of UV-B radiation in plants

English title Perception and signalling of UV-B radiation in plants
Applicant Ulm Roman
Number 132902
Funding scheme Project funding
Research institution Département de Biologie Végétale Faculté des Sciences Université de Genève
Institution of higher education University of Geneva - GE
Main discipline Genetics
Start/End 01.06.2011 - 30.11.2014
Approved amount 468'000.00
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All Disciplines (2)

Discipline
Genetics
Botany

Keywords (5)

UV-B; photomorphogenesis; Arabidopsis; stress acclimation; photoreceptor

Lay Summary (English)

Lead
Lay summary
Ultraviolet-B is a key environmental signal that is specifically perceived by the UVR8 protein in plants and promotes UV acclimation and survival in sunlight. Recent successes have significantly improved our understanding of the function of the UV-B perception pathway and its underlying molecular mechanisms. In particular, photomorphogenic UV-B signalling is mediated by the ?-propeller protein UV RESISTANCE LOCUS 8 (UVR8) and the E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1), involving their early and UV-B-dependent interaction. UVR8 and COP1 are both required for transcriptional activation of the ELONGATED HYPOCOTYL 5 (HY5) gene under UV-B, which encodes a bZIP transcription factor that regulates target genes involved in photoregulatory UV-B responses, including those required for UV-B acclimation. However, our understanding of the UV-B sensory pathway is presently limited to a small number of major players, and even less is known on their interplay, regulations and mechanisms of action. The major aim of this project is a better understanding of UV-B perception and signalling in plants that leads to UV-B acclimation essential for survival.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Publications

Publication
Two distinct domains of the UVR8 photoreceptor interact with COP1 to initiate UV-B signaling in Arabidopsis.
Yin Ruohe, Arongaus Adriana B, Binkert Melanie, Ulm Roman (2015), Two distinct domains of the UVR8 photoreceptor interact with COP1 to initiate UV-B signaling in Arabidopsis., in The Plant Cell, 27(1), 202-13.
Photoreceptor-mediated bending towards UV-B in Arabidopsis.
Vandenbussche Filip, Tilbrook Kimberley, Fierro Ana Carolina, Marchal Kathleen, Poelman Dirk, Van Der Straeten Dominique, Ulm Roman (2014), Photoreceptor-mediated bending towards UV-B in Arabidopsis., in Molecular plant, 7, 1041-1052.
UV-B-responsive association of the Arabidopsis bZIP transcription factor ELONGATED HYPOCOTYL5 with target genes, including its own promoter.
Binkert Melanie, Kozma-Bognár László, Terecskei Kata, De Veylder Lieven, Nagy Ferenc, Ulm Roman (2014), UV-B-responsive association of the Arabidopsis bZIP transcription factor ELONGATED HYPOCOTYL5 with target genes, including its own promoter., in The Plant Cell, 26(10), 4200-4213.
ATR and MKP1 play distinct roles in response to UV-B stress in Arabidopsis.
González Besteiro Marina A, Ulm Roman (2013), ATR and MKP1 play distinct roles in response to UV-B stress in Arabidopsis., in The Plant Journal, 73(6), 1034-1043.
Constitutively active UVR8 photoreceptor variant in Arabidopsis.
Heijde Marc, Binkert Melanie, Yin Ruohe, Ares-Orpel Florence, Rizzini Luca, Van De Slijke Eveline, Persiau Geert, Nolf Jonah, Gevaert Kris, De Jaeger Geert, Ulm Roman (2013), Constitutively active UVR8 photoreceptor variant in Arabidopsis., in Proceedings of the National Academy of Sciences of the United States of America, 110(50), 20326-20331.
Multi-chromatic control of mammalian gene expression and signaling.
Müller Konrad, Engesser Raphael, Schulz Simon, Steinberg Thorsten, Tomakidi Pascal, Weber Cornelia C, Ulm Roman, Timmer Jens, Zurbriggen Matias D, Weber Wilfried (2013), Multi-chromatic control of mammalian gene expression and signaling., in Nucleic Acids Research, 41(12), e124.
Phosphorylation and stabilization of Arabidopsis MAP kinase phosphatase 1 in response to UV-B stress.
González Besteiro Marina A, Ulm Roman (2013), Phosphorylation and stabilization of Arabidopsis MAP kinase phosphatase 1 in response to UV-B stress., in The Journal of Biological Chemistry, 288(1), 480-486.
Reversion of the Arabidopsis UV-B photoreceptor UVR8 to the homodimeric ground state.
Heijde Marc, Ulm Roman (2013), Reversion of the Arabidopsis UV-B photoreceptor UVR8 to the homodimeric ground state., in Proceedings of the National Academy of Sciences of the United States of America, 110(3), 1113-1118.
The UVR8 UV-B photoreceptor: perception, signaling and response
Tilbrook Kimberley, Arongaus Adriana B., Binkert Melanie, Heijde Marc, Yin Ruohe, Ulm Roman (2013), The UVR8 UV-B photoreceptor: perception, signaling and response, in The Arabidopsis Book, 11, e0164.
Ultraviolet-B-mediated induction of protein-protein interactions in mammalian cells.
Crefcoeur Remco P, Yin Ruohe, Ulm Roman, Halazonetis Thanos D (2013), Ultraviolet-B-mediated induction of protein-protein interactions in mammalian cells., in Nature Communications, 4, 1779.
UV-B photoreceptor-mediated signalling in plants
Heijde M, Ulm R (2012), UV-B photoreceptor-mediated signalling in plants, in Trends in Plant Science, 17(4), 230-237.
Arabidopsis MAP kinase phosphatase 1 and its target MAP kinases 3 and 6 antagonistically determine UV-B stress tolerance, independent of the UVR8 photoreceptor pathway
González Besteiro MA, Bartels S, Albert A, Ulm R (2011), Arabidopsis MAP kinase phosphatase 1 and its target MAP kinases 3 and 6 antagonistically determine UV-B stress tolerance, independent of the UVR8 photoreceptor pathway, in PLANT JOURNAL, 68(4), 727-737.
Light-regulated interactions with SPA proteins underlie cryptochrome-mediated gene expression.
Fankhauser Christian, Ulm Roman (2011), Light-regulated interactions with SPA proteins underlie cryptochrome-mediated gene expression., in Genes & development, 25(10), 1004-1009.

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
16th International Congress on Photobiology Talk given at a conference UV-B photoreceptor signalling 08.09.2014 Cordoba, Argentina Ulm Roman;
Photoreceptors: novel systems and new methodologies Talk given at a conference Regulation of the UV-B photoreceptor UVR8, and possible applications 22.09.2013 Schloss Ringberg, Germany Ulm Roman;
15th Congress of the European Society for Photobiology Talk given at a conference UV-B photoreceptor signalling 02.09.2013 Liège, Belgium Ulm Roman;
International Symposium on Plant Photobiology (ISPP) Talk given at a conference Regulation of the UV-B Photoreceptor UVR8 03.06.2013 Edinburgh, Great Britain and Northern Ireland Ulm Roman;
10th International Congress on Plant Molecular Biology (IPMB) Talk given at a conference Regulation of the UV-B photoreceptor UVR8 21.10.2012 Jeju, Korean Republic (South Korea) Ulm Roman;
Photosensory Receptors & Signal Transduction Gordon Research Conference Talk given at a conference UVR8-mediated UV-B perception and signalling in Arabidopsis 22.01.2012 Galveston, TX, United States of America Ulm Roman;
12th International Symposium on Plant Protein Phosphorylation Talk given at a conference Arabidopsis MAP kinase phosphatase 1 determines UV-B stress tolerance 14.09.2011 Tübingen, Germany Ulm Roman;
The 2011 International Symposium on Plant Photobiology (ISPP) Talk given at a conference UV-B perception and signalling in Arabidopsis 02.07.2011 Beijing, China Ulm Roman;


Self-organised

Title Date Place
Plant Light Responses 25.08.2011 Neuchâtel, Switzerland

Associated projects

Number Title Start Funding scheme
153475 Perception and signalling of UV-B radiation in plants 01.12.2014 Project funding

Abstract

Ultraviolet-B is a key environmental signal that is specifically perceived by plants and promotes UV acclimation and survival in sunlight. The UV-B photoreceptor is proposed since over three decades but its identification at the molecular level is still awaited. However, recent successes have significantly improved our understanding of the function of the UV-B perception pathway and its underlying molecular mechanisms. In particular, photomorphogenic UV-B signalling is mediated by the ß-propeller protein UV RESISTANCE LOCUS 8 (UVR8) and the E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1), involving their early and UV-B-dependent interaction. UVR8 and COP1 are both required for transcriptional activation of the ELONGATED HYPOCOTYL 5 (HY5) gene under UV-B, which encodes a bZIP transcription factor that regulates target genes involved in photoregulatory UV-B responses, including those required for UV-B acclimation. However, our understanding of the UV-B sensory pathway is presently limited to a small number of major players, and even less is known on their interplay, regulations and mechanisms of action. The major aim of this proposal is a better understanding of UV-B perception and signalling in plants that leads to UV-B acclimation essential for survival. The three specific major aims of this project are: 1) to understand the regulation of UV-B-induced photomorphogenesis by the UVR8-COP1-HY5 pathway, 2) to understand the structure-function relationship of the UVR8 protein as the primary UV-B photoreceptor candidate, 3) to determine the mechanism of action of the novel UVR8-regulators REPRESSOR OF UV-B PHOTOMORPHOGENESIS (RUP) 1 and 2.
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