Neurobiology; Neuroscience; Behavior; Thermosensation; Thermal Nociception; Thermonociception; C. elegans; Worm; neurosciences; genetics; worm; Caenorhabditis elegans; nociception; pain; temperature sensation; behavior; escape; avoidance; optogenetics; channelrhodopsin; archeorhodopsin; caspase; neuronal ablation; neural circuit; quantitative analyses of behavior; mutagenesis screen; whole genome sequencing
Glauser Dominique (2015), Dual Color Neural Activation and Behavior Control with Chrimson and CoChR in Caenorhabditis elegans., in Genetics
Glauser Dominique (2014), A conserved role for p48 homologs in protecting dopaminergic neurons from oxidative stress., in PLOS Genetics
Glauser Dominique (2014), CaMKI-dependent regulation of sensory gene expression mediates experience-dependent plasticity in the operating range of a thermosensory neuron., in Neuron
Glauser Dominique (2014), The balance between cytoplasmic and nuclear CaM kinase-1 signaling controls the operating range of noxious heat avoidance., in Neuron
Rufener Lucien, Bedoni Nicola, Baur Roland, Rey Samantha, Glauser Dominique A., Bouvier Jacques, Beech Robin, Sigel Erwin, Puoti Alessandro (2013), acr-23 Encodes a Monepantel-Sensitive Channel in Caenorhabditis elegans, in PLOS PATHOGENS
, 9(8), 1003524.
Schild Lisa C., Glauser Dominique A. (2013), Dynamic switching between escape and avoidance regimes reduces Caenorhabditis elegans exposure to noxious heat, in NATURE COMMUNICATIONS
, 4, 3198.
Glauser Dominique A. (2013), How and why Caenorhabditis elegans uses distinct escape and avoidance regimes to minimize exposure to noxious heat, in Worm
, 2(4), e27285.
Glauser Dominique A, Chen Will C, Agin Rebecca, Macinnis Bronwyn L, Hellman Andrew B, Garrity Paul A, Tan Man-Wah, Goodman Miriam B (2011), Heat avoidance is regulated by transient receptor potential (TRP) channels and a neuropeptide signaling pathway in Caenorhabditis elegans., in Genetics
, 188(1), 91-103.
Glauser DA, Johnson BE, Aldrich RW, Goodman MB (2011), Intragenic alternative splicing coordination is essential for Caenorhabditis elegans slo-1 gene function, in PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
, 108(51), 20790-20795.
The project aims at developing the use of C. elegans model organism to study temperature and pain sensation. Most C. elegans genes involved in temperature/pain sensation that we discovered so far are conserved in human, suggesting that our studies may bring insights on novel drug targets in pain management. Significant progresses made so far include:1) Methodology : we developed (i) thermo-electrical apparatus to control heat stimuli in time and space, (ii) a computer-assisted analysis protocol providing a quantitative and objective assessment of behavior, (iii) light-stimulus system to control channelrhodopsin and other light activated-proteins, (iv) a recombinase-based approach to target transgene expression in single neurons.2) Findings: through the systematic and combinatorial study of mutations, we were able to draft a map of the gene interaction network regulating temperature/pain sensation. Furthermore, thanks to those mutations and neuron specific "genetic rescue" experiments, we also obtained key information on the neural circuit regulating temperature/pain sensation and on the execution of heat avoidance behaviors. Specifically, we are now able to link specific neurons class to specific behavioral components.