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Nanostructured Polymers

English title Nanostructured Polymers
Applicant Meier Wolfgang
Number 131884
Funding scheme Project funding
Research institution Physikalische Chemie Departement Chemie Universität Basel
Institution of higher education University of Basel - BS
Main discipline Physical Chemistry
Start/End 01.10.2010 - 30.09.2013
Approved amount 575'394.00
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Keywords (5)

polymer synthesis; self assembly; nanostructures; vesicles; amphiphilic block copolymers

Lay Summary (English)

Lead
Lay summary

Self-assembly of amphiphilic block copolymers in aqueous media is a powerful tool for the development of new, soft materials that are able to express well-defined functions. Here we plan to synthesize new amphiphilic ABC triblock copolymers with an inert, electrically neutral and water- soluble block A and a responsive block C. Structural changes of the self-assembled superstructures will be induced by external triggers and followed in situ. For example, a reversible membrane inversion in ABC triblock copolymer vesicles could indicate thermodynamic stability of such partices. In addition the potential of these systems as triggered release devices for bioactive compounds will be evaluated.

Specific interactions at block copolymer membranes will be systematically studied in solution and at surfaces using recently introduced Ni-NTA functionalized block copolymers. It is expected that the dense array of binding sites at membrane surfaces can induce the formation of highly ordered arrays of his-tagged proteins. Such arrays could find interest as tools in structural biology for membrane protein characterization or as new devices for biosensing. The experiments will serve as a basis to synthesize, characterize and compare also selective binding of folate and mannose terminated polymers that might be interesting for targeted delivery of drugs.

In addition block copolymer vesicles will be used as compartments for the generation of covalently crosslinked hydrogels in their inner aqueous cavity. It is expected that the hydrogels act as a mimic of the cytoskeleton of biological cells and will modify the structure and the mechanical behavior of the vesicles. Responsive hydrogels can be used to trigger structural changes in the system, that will be followed in situ and that could also be used for initiating release of encapsulated materials. Moreover hydrogels will be modified with anchor sites for transmembrane proteins. We expect that successful protein binding to the inner gel will significantly affect the lateral mobility of the proteins in the polymer membranes. First experiments with polymer vesicles loaded with actin filaments would help to take the systems one step further towards a cell mimetic system.

Channel protein equipped block copolymer vesicles (‘nanoreactors’) in solution and at surfaces provide a nanometersized compartmentalization that will be used to study enzymatic reactions down to a single enzyme level. Of particular interest will be to evaluate the effects of spatial confinement and/or molecular crowding inside the nanoreactors on the activity of the encapsulated enzymes.

Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Publications

Publication
A synthetic nanomaterial for virus recognition produced by surface imprinting
Cumbo A., Lorber B., Corvini P. F. X., Meier W., Shahgaldian P. (2013), A synthetic nanomaterial for virus recognition produced by surface imprinting, in Nature Communications, 4, 1.
Biodegradable polymer-lipid monolayers as templates for calcium phosphate mineralization
Jagoda A., Zinn M., Bieler E., Meier W., Kita-Tokarczyk K. (2013), Biodegradable polymer-lipid monolayers as templates for calcium phosphate mineralization, in Journal of Materials Chemistry B, 1, 368-378.
Fluorescence-Based Assay for the Optimization of the Activity of Artificial Transfer Hydrogenase within a Biocompatible Compartment
Heinisch T., Langowska K., Tanner P., Reymond J. L., Meier W., Palivan C., Ward T. R. (2013), Fluorescence-Based Assay for the Optimization of the Activity of Artificial Transfer Hydrogenase within a Biocompatible Compartment, in Chemcatchem, 5, 720-723.
Gel-Assisted Formation of Giant Unilamellar Vesicles
Weinberger A., Tsai F. C., Koenderink G. H., Schmidt T. F., Itri R., Meier W., Schmatko T., Schroder A., Marques C. (2013), Gel-Assisted Formation of Giant Unilamellar Vesicles, in Biophysical Journal, 105, 154-164.
Natural Channel Proteins Inserts and Functions in a Completely Artificial, Solid-supported Bilayer Membrane
Zhang Xiaoyan, Fu Wangyang, Palivan Cornelia G., Meier Wolfgang (2013), Natural Channel Proteins Inserts and Functions in a Completely Artificial, Solid-supported Bilayer Membrane, in Scientific Reports, 3, 2196.
Photoreaction of a Hydroxyalkyphenone with the Membrane of Polymersomes: A Versatile Method To Generate Semipermeable Nanoreactors
Spulber M., Najer A., Winkelbach K., Glaied O., Waser M., Pieles U., Meier W., Bruns N. (2013), Photoreaction of a Hydroxyalkyphenone with the Membrane of Polymersomes: A Versatile Method To Generate Semipermeable Nanoreactors, in Journal of the American Chemical Society, 135, 9204-9212.
Polymer nanocompartment in broad-spectrum medical applications
Najer A., Wu D., Vasquez D., Palivan C. G., Meier W. (2013), Polymer nanocompartment in broad-spectrum medical applications, in Nanomedicine, 8(3), 425.
Polymer nanoreactors
Edlinger Christoph, Zhang Xiaoyan, Fischer-Onaca Ozana, Paliva G. Cornelia (2013), Polymer nanoreactors, in Mark Herman F. (ed.), John Wiley & Sons, Inc., Eupropa, 1-8.
Polymer nanoreactors shown to produce and release antibiotics locally
Langowska K., Palivan C. G., Meier W. (2013), Polymer nanoreactors shown to produce and release antibiotics locally, in Chemical Communications, 49, 128-130.
Polymer Vesikel auf Oberflächen
Jagoda Agnieszka, Kowal Justyna, Delcea Mihaela, PalivanCornelia G., Meier Wolfgang (2013), Polymer Vesikel auf Oberflächen, in Mano Andreas Taubert João F. (ed.), Wiley-VCH Verlag GmbH & Co. KGaA, Eupropa, 159.
Printing enzymes for Colorimetric assays on papers
Saxer. S. S., Wildi F., Walser W., Boss O., Zimmer R., Spulber M., Meier W., Paul P., Bruns N., Pieles U. (2013), Printing enzymes for Colorimetric assays on papers, in Chimia, 67, 648.
Protein–Polymer Supramolecular Assemblies: A Key Combination for Multifunctionality
Palivan Cornelia G., Zhang Xiaoyan, Meier Wolfgang (2013), Protein–Polymer Supramolecular Assemblies: A Key Combination for Multifunctionality, in Chimia, 87, 791-795.
Self-assembled peptide beads used as a template for ordered gold nanoparticle superstructures
de Bruyn Ouboter D., Spulber M., Sigg S. T., Meier W. (2013), Self-assembled peptide beads used as a template for ordered gold nanoparticle superstructures, in Colloid Suef. B: Biointerfaces, 112, 542-547.
Tuning the resistance of polycarbonate membranes by plasma-induced graft surface modification
Baumann L., Hegemann D., de Courten D., Wolf M., Rossi R. M., Meier W. P., Scherer L. J. (2013), Tuning the resistance of polycarbonate membranes by plasma-induced graft surface modification, in Applied Surface Science, 268, 450-457.
A novel type of silver nanoparticles and their advantages in toxicity testing in cell culture systems
Haase A., Mantion A., Graf P., Plendl J., Thuenemann A. F., Meier W., Taubert A., Luch A. (2012), A novel type of silver nanoparticles and their advantages in toxicity testing in cell culture systems, in Archives of Toxicology, 86, 1089-1098.
Benzodifuran-containing well-defined pi-conjugated polymers for photovoltaic cells
Li Hui, Tang Peng, Zhao Yan, Liu Shi-Xia, Aeschi Yves, Deng Lijun, Braun Joerg, Zhao Bin, Liu Yunqi, Tan Songting, Meier Wolfgang, Decurtins Silvio (2012), Benzodifuran-containing well-defined pi-conjugated polymers for photovoltaic cells, in Journal of Polymer Science Part a-Polymer Chemistry, 50, 2935-2943.
CO2 permeability of cell membranes is regulated by membrane cholesterol and protein gas channels
Itel Fabian, Al-Samir Samer, Öberg Fredrik, Chami Mohamed, Kumar Manish, Supuran Claudiu T., Deen Peter M. T., Meier Wolfgang, Hedfalk Kristina, Gros Gerolf, Endeward Volker (2012), CO2 permeability of cell membranes is regulated by membrane cholesterol and protein gas channels, in The FASEB Journal, 26, 1-4.
Effects of Silver Nanoparticles on Primary Mixed Neural Cell Cultures: Uptake, Oxidative Stress and Acute Calcium Responses
Haase Andrea, Rott Stephanie, Mantion Alexandre, Graf Philipp, Plendl Johanna, Thuenemann Andreas F., Meier Wolfgang P., Taubert Andreas, Luch Andreas, Reiser Georg (2012), Effects of Silver Nanoparticles on Primary Mixed Neural Cell Cultures: Uptake, Oxidative Stress and Acute Calcium Responses, in Toxicological Sciences, 126, 457-468.
Gas-tight triblock-copolymer membranes are converted to CO2 permeable by insertion of plant aquaporins
Uehlein Norbert, Otto Beate, Eilingsfeld Adrian, Itel Fabian, Meier Wolfgang, Kaldenhoff Ralf (2012), Gas-tight triblock-copolymer membranes are converted to CO2 permeable by insertion of plant aquaporins, in Scientific Reports, 2, 1-4.
Head Group Influence on Lipid Interactions With a Polyhydroxyalkanoate Biopolymer
Jagoda Agnieszka, Zinn Manfred, Meier Wolfgang, Kita-Tokarczyk Katarzyna (2012), Head Group Influence on Lipid Interactions With a Polyhydroxyalkanoate Biopolymer, in Macromolecular Chemistry and Physics, 213, 1922-1932.
High-Density Reconstitution of Functional Water Channels into Vesicular and Planar Block Copolymer Membranes
Kumar M., Habel J. E. O., Shen Y. X., Meier W. P., Walz T. (2012), High-Density Reconstitution of Functional Water Channels into Vesicular and Planar Block Copolymer Membranes, in Journal of the American Chemical Society, 134, 18631-18637.
Highly Permeable and Selective Pore-Spanning Biomimetic Membrane Embedded with Aquaporin Z
Wang Honglei, Chung Tai-Shung, Tong Yen Wah, Jeyaseelan Kandiah, Armugam Arunmozhiarasi, Chen Zaichun, Hong Minghui, Meier Wolfgang (2012), Highly Permeable and Selective Pore-Spanning Biomimetic Membrane Embedded with Aquaporin Z, in Small, 8, 1185-1190.
Membrane protein distribution in composite polymer-lipid thin films
Thoma Johannes, Belegrinou Serena, Rossbach Peggy, Grzelakowski Mariusz, Kita-Tokarczyk Katarzyna, Meier Wolfgang (2012), Membrane protein distribution in composite polymer-lipid thin films, in Chemical Communications, 48, 8811-8813.
Mimicking the cell membrane with block copolymer membranes
Zhang Xiaoyan, Tanner Pascal, Graff Alexandra, Palivan Cornelia G., Meier Wolfgang (2012), Mimicking the cell membrane with block copolymer membranes, in Journal of Polymer Science Part a-Polymer Chemistry, 50, 2293-2318.
Probing Bioinspired Transport of Nanoparticles into Polymersomes
Jaskiewicz Karmena, Larsen Antje, Lieberwirth Ingo, Koynov Kaloian, Meier Wolfgang, Fytas George, Kroeger Anja, Landfester Katharina (2012), Probing Bioinspired Transport of Nanoparticles into Polymersomes, in Angewandte Chemie-International Edition, 51, 4613-4617.
Solution Behavior of Double-Hydrophilic Block Copolymers in Dilute Aqueous Solution
Casse Olivier, Shkilnyy Andriy, Linders Juergen, Mayer Christian, Haeussinger Daniel, Voelkel Antje, Thuenemann Andreas F., Dimova Rumiana, Coelfen Helmut, Meier Wolfgang, Schlaad Helmut, Taubert Andreas (2012), Solution Behavior of Double-Hydrophilic Block Copolymers in Dilute Aqueous Solution, in Macromolecules, 45, 4772-4777.
Specific His(6)-tag Attachment to Metal-Functionalized Polymersomes Relies on Molecular Recognition
Tanner Pascal, Ezhevskaya Maria, Nehring Rainer, Van Doorslaer Sabine, Meier Wolfgang, Palivan Cornelia (2012), Specific His(6)-tag Attachment to Metal-Functionalized Polymersomes Relies on Molecular Recognition, in Journal of Physical Chemistry B, 116, 10113-10124.
Stimuli-Responsive Polymers and Their Applications in Nanomedicine
Cabane Etienne, Zhang Xiaoyan, Langowska Karolina, Palivan Cornelia G., Meier Wolfgang (2012), Stimuli-Responsive Polymers and Their Applications in Nanomedicine, in Biointerphases, 7, 1-27.
Synthetic biology, inspired by synthetic chemistry
Malinova V., Nallani M., Meier W. P., Sinner E. K. (2012), Synthetic biology, inspired by synthetic chemistry, in Febs Letters, 586, 2146-2156.
Amphiphilic PEG-b-PMCL-b-PDMAEMA Triblock Copolymers: From Synthesis to Physico-Chemistry of Self-Assembled Structures
Matter Y., Enea R., Casse O., Lee C. C., Baryza J., Meier W. (2011), Amphiphilic PEG-b-PMCL-b-PDMAEMA Triblock Copolymers: From Synthesis to Physico-Chemistry of Self-Assembled Structures, in Macromolecular Chemistry and Physics, 212(9), 937-949.
Application of Laser Postionization Secondary Neutral Mass Spectrometry/Time-of-Flight Secondary Ion Mass Spectrometry in Nanotoxicology: Visualization of Nanosilver in Human Macrophages and Cellular Responses
Haase A., Arlinghaus H. F., Tentschert J., Jungnickel H., Graf P., Mantion A., Draude F., Galla S., Plendl J., Goetz M. E., Masic A., Meier W., Thunemann A. F., Taubert A., Luch A. (2011), Application of Laser Postionization Secondary Neutral Mass Spectrometry/Time-of-Flight Secondary Ion Mass Spectrometry in Nanotoxicology: Visualization of Nanosilver in Human Macrophages and Cellular Responses, in Acs Nano, 5(4), 3059-3068.
Aquaporin based triblock copolymer membranes for water purification
Kumar M., Meier W., Zilles J. (2011), Aquaporin based triblock copolymer membranes for water purification, in Polymer Preprints, 52(2), 1060-1065.
Biocompatible Functionalization of Polymersome Surfaces: A New Approach to Surface Immobilization and Cell Targeting Using Polymersomes
Egli S., Nussbaumer M. G., Balasubramanian V., Chami M., Bruns N., Palivan C., Meier W. (2011), Biocompatible Functionalization of Polymersome Surfaces: A New Approach to Surface Immobilization and Cell Targeting Using Polymersomes, in Journal of the American Chemical Society, 133(12), 4476-4483.
Biomimetic synthesis of chiral erbium-doped silver/peptide/silica core-shell nanoparticles (ESPN)
Mantion Alexandre, Graf Philipp, Florea Ileana, Haase Andrea, Thuenemann Andreas F., Masic Admir, Ersen Ovidiu, Rabu Pierre, Meier Wolfgang, Luch Andreas, Taubert Andreas (2011), Biomimetic synthesis of chiral erbium-doped silver/peptide/silica core-shell nanoparticles (ESPN), in Nanoscale, 3, 5168-5179.
Can polymeric vesicles that confine enzymatic reactions act as simplified organelles?
Tanner P., Egli S., Balasubramanian V., Onaca O., Palivan C. G., Meier W. (2011), Can polymeric vesicles that confine enzymatic reactions act as simplified organelles?, in Febs Letters, 585(11), 1699-1706.
Enzymatic Cascade Reactions inside Polymeric Nanocontainers: A Means to Combat Oxidative Stress
Tanner P., Onaca O., Balasubramanian V., Meier W., Palivan C. G. (2011), Enzymatic Cascade Reactions inside Polymeric Nanocontainers: A Means to Combat Oxidative Stress, in Chemistry-a European Journal, 17(16), 4552-4560.
Exploiting dimerization of amphiphilic peptides to form vesicles
Schuster Th. B., Ouboter D. de Bruyn, Bruns N., Meier W. (2011), Exploiting dimerization of amphiphilic peptides to form vesicles, in Small, 7(15), 2158-2160.
From Fibers to Micelles Using Point-Mutated Amphiphilic Peptides
Schuster T. B., Ouboter D. D., Palivan C. G., Meier W. (2011), From Fibers to Micelles Using Point-Mutated Amphiphilic Peptides, in Langmuir, 27(8), 4578-4584.
Functionalization of Block Copolymer Vesicle Surfaces
Egli S., Schlaad H., Bruns N., Meier W. (2011), Functionalization of Block Copolymer Vesicle Surfaces, in Polymers, 3(1), 252-280.
Hierarchical Organization of Purely Peptidic Amphiphiles into Peptide Beads
Ouboter D. D., Schuster T. B., Mantion A., Meier W. (2011), Hierarchical Organization of Purely Peptidic Amphiphiles into Peptide Beads, in Journal of Physical Chemistry C, 115(30), 14583-14590.
Interactions of biodegradable poly([R]-3-hydroxy-10-undecenoate) with DOPC lipid: a monolayer study
Jagoda A., Ketikidis P., Zinn M., Meier W., Kita-Tokarczyk K. (2011), Interactions of biodegradable poly([R]-3-hydroxy-10-undecenoate) with DOPC lipid: a monolayer study, in Langmuir, 27, 10878-10885.
Phase Behavior of Vesicle-Forming Block Copolymers in Aqueous Solutions
Braun J., Bruns N., Pfohl T., Meier W. (2011), Phase Behavior of Vesicle-Forming Block Copolymers in Aqueous Solutions, in Macromolecular Chemistry and Physics, 212(12), 1245-1254.
Photoresponsive polymersomes as smart, triggerable nanocarriers
Cabane E., Malinova V., Menon S., Palivan C. G., Meier W. (2011), Photoresponsive polymersomes as smart, triggerable nanocarriers, in Soft Matter, 7(19), 9167-9176.
Planar Block Copolymer Membranes by Vesicle Spreading
Dorn J., Belegrinou S., Kreiter M., Sinner E. K., Meier W. (2011), Planar Block Copolymer Membranes by Vesicle Spreading, in Macromolecular Bioscience, 11(5), 514-525.
Polymer Membranes
Meier W., Palivan C. (2011), Polymer Membranes, in Chimia, 64, 880-883.
Polymeric Vesicles: From Drug Carriers to Nanoreactors and Artificial Organelles
Tanner Pascal, Baumann Patric, Enea Ramona, Onaca Ozana, Palivan Cornelia, Meier Wolfgang (2011), Polymeric Vesicles: From Drug Carriers to Nanoreactors and Artificial Organelles, in Accounts of Chemical Research, 44, 1039-1049.
Preparation and characterization of pore-suspending biomimetic membranes embedded with Aquaporin Z on carboxylated polyethylene glycol polymer cushion
Wang H. L., Chung T. S., Tong Y. W., Meier W., Chen Z. C., Hong M. H., Jeyaseelan K., Armugam A. (2011), Preparation and characterization of pore-suspending biomimetic membranes embedded with Aquaporin Z on carboxylated polyethylene glycol polymer cushion, in Soft Matter, 7(16), 7274-7180.
Protein-polymer nanoreactors for medical applications
Palivan Cornelia G., Fischer-Onaca Ozana, Delcea Mihaela, Itel Fabian, Meier Wolfgang (2011), Protein-polymer nanoreactors for medical applications, in Chemical Society Reviews, 41, 2800-2823.
Selective and Responsive Nanoreactors
Renggli K., Baumann P., Langowska K., Onaca O., Bruns N., Meier W. (2011), Selective and Responsive Nanoreactors, in Advanced Functional Materials, 21(7), 1241-1259.
Silicification of Peptide-Coated Silver Nanoparticles-A Biomimetic Soft Chemistry Approach toward Chiral Hybrid Core-Shell Materials
Graf P., Mantion A., Haase A., Thunemann A. F., Masic A., Meier W., Luch A., Taubert A. (2011), Silicification of Peptide-Coated Silver Nanoparticles-A Biomimetic Soft Chemistry Approach toward Chiral Hybrid Core-Shell Materials, in Acs Nano, 5(2), 820-833.
Solid-supported polymeric membranes
Belegrinou S., Menon S., Dobrunz D., Meier W. (2011), Solid-supported polymeric membranes, in Soft Matter, 7(6), 2202-2210.
Synthesis of poly(styrene-co-diene)-block-polyglycidol. Self-association and stabilization of aggregates
Haladjova E., Dishovsky N., Meier W., Tsvetanov C. B., Novakov C. P. (2011), Synthesis of poly(styrene-co-diene)-block-polyglycidol. Self-association and stabilization of aggregates, in Soft Matter, 7(19), 9459-9467.
Synthetic polyion-counterion transport systems in polymersomes and gels
Montenegro J., Braun J., Fischer-Onaca O., Meier W., Matile S. (2011), Synthetic polyion-counterion transport systems in polymersomes and gels, in Organic & Biomolecular Chemistry, 9(19), 6623-6628.
Benzodifuran-Based pi-Conjugated Copolymers for Bulk Heterojunction Solar Cells
Li H., Jiang P., Yi C. Y., Li C., Liu S. X., Tan S. T., Zhao B., Braun J., Meier W., Wandlowski T., Decurtins S. (2010), Benzodifuran-Based pi-Conjugated Copolymers for Bulk Heterojunction Solar Cells, in Macromolecules, 43(19), 8058-8062.
Poly(ethylene oxide)-poly(ethylene imine) block copolymers as templates and catalysts for the in situ formation of monodisperse silica nanospheres
Kind L., Shkilnyy A., Schlaad H., Meier W., Taubert A. (2010), Poly(ethylene oxide)-poly(ethylene imine) block copolymers as templates and catalysts for the in situ formation of monodisperse silica nanospheres, in Colloid and Polymer Science, 288(16-17), 1645-1650.
Reversible peptide particle formation using a mini amino acid sequence
Schuster T. B., Ouboter D. D., Bordignon E., Jeschke G., Meier W. (2010), Reversible peptide particle formation using a mini amino acid sequence, in Soft Matter, 6(21), 5596-5604.
Solid Peptide Nanoparticles - Structural Characterization and Quantification of Cargo Encapsulation
Dittrich C., Meier W. (2010), Solid Peptide Nanoparticles - Structural Characterization and Quantification of Cargo Encapsulation, in Macromolecular Bioscience, 10(12), 1406-1415.
Towards Targeted Drug Delivery by Covalent Ligand-Modified Polymeric Nanocontainers
Egli S., Fischer B., Hartmann S., Hunziker P., Meier W., Rigler P. (2010), Towards Targeted Drug Delivery by Covalent Ligand-Modified Polymeric Nanocontainers, in Modern Trends in Polymer Science-Epf 09, 296, 278-285.

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
Fall Meeting of the Swiss Chemical Society 2013 Poster Poly (N-isopropylacrylamide-co-tris-nitrilotriacetic acid acrylamide) to serve for molecular recognition 06.09.2013 Lausanne, Switzerland Meier Wolfgang;
Swiss Chemical Society Fall Meeting Poster Solid-supported planar polymer membranes as the platforms for protein incorporation 06.09.2013 Lausanne, Switzerland Meier Wolfgang;
Swiss Chemical Society Fall Meeting Poster pH responsive PEG-b-PMCL-b-PDMAEMA triblock copolymers for protein therapy 06.09.2013 Lausanne, Switzerland Meier Wolfgang; Vasquez Munoz Daniela;
Swiss Chemical Society Fall Meeting Poster Natural channel protein inserts and functions in an artificial, solid-supported bilayer membrane 06.09.2013 Lausanne, Switzerland Meier Wolfgang;
PolyColl 2013, Annual Meeting of the Division of Polymers, Colloids and Interfaces Poster Self-assembling behaviour of diblock PDMS-PMOXA polymers: structure influence 07.07.2013 Basel, Switzerland Meier Wolfgang;
PolyColl Poster Solid-supported planar polymer membranes for biosensing applications 07.07.2013 Basel, Switzerland Meier Wolfgang;
PolyColl Poster Natural channel protein inserts and functions in an artificial, solid-supported bilayer membrane 07.07.2013 Basel, Switzerland Meier Wolfgang;
European Polymer Congress Poster Solid-supported planar polymer membranes for biosensing applications 16.06.2013 Pisa, Italy Meier Wolfgang;
NRP 62 kick-off meeting Talk given at a conference Functional surfaces through immobilization of nanoreactors and biomimetic block copolymer membranes 30.05.2013 Muntelier, Switzerland Meier Wolfgang;
Swiss NanoConvention Poster Natural channel protein inserts and functions in an artificial, solid-supported bilayer membrane 23.05.2013 Basel, Switzerland Meier Wolfgang;
Third International Symposium Frontiers in Polymer Science Poster Self-assembling behavior of PDMS-b-PMOXA diblock polymers: structure influence 21.05.2013 Sitges , Spain Meier Wolfgang;
Frontiers in Polymer Science Poster Poly (N-isopropylacrylamide-co-tris-nitrilotriacetic acid acrylamide) for molecular recognition and protein immobilization 21.05.2013 Sitges, Spain Meier Wolfgang;
Frontiers in Polymer Science Poster pH responsive PEG-b-PMCL-b-PDMAEMA triblock copolymers for protein therapy 08.05.2013 Sitges, Spain Meier Wolfgang; Vasquez Munoz Daniela;
EuroSYNBIO Poster Biomimetic Solid-supported Polymer Bilayer Incorporated with Membrane Proteins 06.05.2013 Elmau, Germany Meier Wolfgang;
NanoEvent “NextNanoStars” Poster Bio-inspired amphiphilic block copolymers for biosensing and drug delivery 21.03.2013 Basel, Switzerland Meier Wolfgang;
Swiss Soft Days (10th Edition) Poster Poly (N-isopropylacrylamide-co-tris-nitrilotriacetic acid acrylamide) to serve for molecular recognition 04.03.2013 Paul Scherrer Institut, Switzerland Meier Wolfgang;
Macromolecular Colloquium Poster Biomimetic Solid-supported Polymer Bilayer Incorporated with Membrane Proteins 21.02.2013 Freiburg, Germany Meier Wolfgang;
Rigi Workshop Poster Biomimetic Solid-supported Polymer Bilayer Incorporated with Membrane Proteins 20.01.2013 Rigi, Switzerland Meier Wolfgang;
Swiss Soft Days 9th Edition Poster PDMS-PMOXA diblock copolymer synthesis and its self-assembly behavior in water 29.10.2012 Lausanne, Switzerland Meier Wolfgang;
Swiss Soft Days (9th Edition) Poster Poly (N-isopropylacrylamide-co-tris-nitrilotriacetic acid acrylamide) to serve for molecular recognition 29.10.2012 Lausanne, Switzerland Meier Wolfgang;
Swiss Soft Says 9th Edition Poster Probing Membrane Proteins Incorporation into A Biomimetic Solid-supported Polymer Bilayer 29.10.2012 Lausanne, Switzerland Meier Wolfgang;
EuroSYNBIO Poster Probing Membrane Proteins Incorporation into A Biomimetic Solid-supported Polymer Bilayer 03.10.2012 Groningen, Netherlands Meier Wolfgang;


Communication with the public

Communication Title Media Place Year
Talks/events/exhibitions Sicence Slam No. 2 Basel German-speaking Switzerland 2012
Talks/events/exhibitions Weltenreise German-speaking Switzerland 2011

Associated projects

Number Title Start Funding scheme
117572 Nanostrukturierte Polymere 01.10.2007 Project funding
150059 Nanostructured Polymers 01.10.2013 Project funding
126099 Functional surfaces through immobilization of nanoreactors and biomimetic block copolymer membranes 01.01.2010 NRP 62 Smart Materials
163996 Interactions, Dynamics, and Functionality at Nanoscale Characterised by Confocal Laser Scanning Microscopy and Fluorescence Correlation Spectroscopy 01.12.2015 R'EQUIP

Abstract

Self-assembly of amphiphilic block copolymers in aqueous media is a powerful tool for the development of new, soft materials that are able to express well-defined functions. Here we plan to synthesize new amphiphilic ABC triblock copolymers with an inert, electrically neutral and water-soluble block A and a responsive block C. Structural changes of the self-assembled superstructures will be induced by external triggers and followe in situ. For example, a reversible membrane inversion in ABC triblock copolymer vesicles could indicate thermodynamic stability of such partices. In addition the potential of these systems as triggered release devices for bioactive compounds will be evaluated. Specific interactions at block copolymer membranes will be systematically studied in solution and at surfaces using recently introduced Ni-NTA functionalized block copolymers. It is expected that the dense array of binding sites at membrane surfaces can induce the formation of highly ordered arrays of his-tagged proteins. Such arrays could find interest as tools in structural biology for membrane protein characterization or as new devices for biosensing. The experiments will serve as a basis to synthesize, characterize and compare also selective binding of folate and mannose terminated polymers that might be interesting for targeted delivery of drugs.In addition block copolymer vesicles will be used as compartments for the generation of covalently crosslinked hydrogels in their inner aqueous cavity. It is expected that the hydrogels act as a mimic of the cytoskeleton of biological cells and will modify the structure and the mechanical behavior of the vesicles. Responsive hydrogels can be used to trigger structural changes in the system, that will be followed in situ and that could also be used for initiating release of encapsulated materials. Moreover hydrogels will be modified with anchor sites for transmembrane proteins. We expect that successful protein binding to the inner gel will significantly affect the lateral mobility of the proteins in the polymer membranes. First experiments with polymer vesicles loaded with actin filaments would help to take the systems one step further towards a cell mimetic system.Channel protein equipped block copolymer vesicles (‘nanoreactors’) in solution and at surfaces provide a nanometersized compartmentalization that will be used to study enzymatic reactions down to a single enzyme level. Of particular interest will be to evaluate the effects of spatial confinement and/or molecular crowding inside the nanoreactors on the activity of the encapsulated enzymes.
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