regeneration; stem cells; developmental plasticity; RNA interference; high throughput sequencing; MAPK signaling pathway; WNt signaling pathway; gene regulatory network; cellular remodeling; hydra model system; evolution; hydra; cellular remodelling; homeostasis; injury; growth control; patterning; apoptosis-induced compensatory proliferation; gene regulatory networks; signaling
Galliot Brigitte (2013), Injury-induced asymmetric cell death as a driving force for head regeneration in Hydra., in Development genes and evolution
, 223(1-2), 39-52.
Wenger Yvan, Galliot Brigitte (2013), Punctuated Emergences of Genetic and Phenotypic Innovations in Eumetazoan, Bilaterian, Euteleostome, and Hominidae Ancestors, in Genome Biology and Evolution
, 5(10), 1949-1968.
Wenger Yvan, Galliot Brigitte (2013), RNAseq versus genome-predicted transcriptomes: a large population of novel transcripts identified in an Illumina-454 Hydra transcriptome., in BMC genomics
, 14, 204-204.
Klionsky Daniel J, Abdalla Fabio C, Abeliovich Hagai, Abraham Robert T, Acevedo-Arozena Abraham, Adeli Khosrow, Agholme Lotta, Agnello Maria, Agostinis Patrizia, Aguirre-Ghiso Julio A, Ahn Hyung Jun, Ait-Mohamed Ouardia, Ait-Si-Ali Slimane, Akematsu Takahiko, Akira Shizuo, Al-Younes Hesham M, Al-Zeer Munir A, Albert Matthew L, Albin Roger L, Alegre-Abarrategui Javier, Aleo Maria Francesca, Alirezaei Mehrdad, Almasan Alexandru, Almonte-Becerril Maylin, Amano Atsuo (2012), Guidelines for the use and interpretation of assays for monitoring autophagy., in Autophagy
, 8(4), 445-544.
Bossert Patricia, Galliot Brigitte (2012), How to use Hydra as a model system to teach biology in the classroom., in The International journal of developmental biology
, 56(6-7-8), 637-652.
Galliot Brigitte (2012), Hydra, a fruitful model system for 270 years., in The International journal of developmental biology
, 56(6-7-8), 411-423.
Reiter Silke, Crescenzi Marco, Galliot Brigitte, Buzgariu Wanda (2012), Hydra, a versatile model to study the homeostatic and developmental functions of cell death., in The International journal of developmental biology
, 56(6-7-8), 593-604.
Galliot Brigitte (2012), The Hydra Model System., in The International journal of developmental biology
, 56(6-7-8), 407-409.
Galliot Brigitte (2011), [Between homeostasis and development, which strategies to regenerate?]., in Biologie aujourd'hui
, 205(2), 125-37.
Galliot Brigitte, Quiquand Manon (2011), A two-step process in the emergence of neurogenesis., in The European journal of neuroscience
, 34(6), 847-62.
Chera Simona, Ghila Luiza, Wenger Yvan, Galliot Brigitte (2011), Injury-induced activation of the MAPK/CREB pathway triggers apoptosis-induced compensatory proliferation in hydra head regeneration., in Development, growth & differentiation
, 53(2), 186-201.
Galliot Brigitte, Ghila Luiza (2010), Cell plasticity in homeostasis and regeneration., in Molecular reproduction and development
, 77(10), 837-55.
Galliot Brigitte, Chera Simona (2010), The Hydra model: disclosing an apoptosis-driven generator of Wnt-based regeneration., in Trends in cell biology
, 20(9), 514-23.
The freshwater polyp hydra is a relatively simple animal currently emerging as a potent model system to study how cell plasticity regulates regeneration. Following mid-gastric bisection, the two hydra halves immediately initiate an asymmetric process leading to the regeneration of the missing structures in less than 3 days, thus leading to the formation of two complete animals. Interestingly, the asymmetry underlying this regenerative process is emerging very rapidly at the molecular and cellular level in two regions that were identical prior to mid-gastric bisection. As an example head regeneration requires controlled apoptosis and compensatory proliferation of distinct cell populations in head-regenerating stumps, whereas foot regeneration occurs in the absence of cell death and cell proliferation. At the signaling level, the asymmetric activation of the MAPK and Wnt3 pathways supports the early head organizer activity (Chera et al., Dev Cell 2009). To identify the genetic mechanisms underlying the establishment of a polarity and the subsequent regeneration process, we launched a RNAi screen that we plan to continue with this project. As a complementary strategy, we also want to perform a high-throughput transcriptome analysis using the illumina/Solexa method. This method allows to take a snapshot of virtually all the transcribed genes in well defined conditions, i.e. here at determined time points during regeneration and at different locations along hydra’s body column. Subsequently, customs microarrays will be produced and mRNAs from regeneration mutants generated through the RNAi feeding technique will be tested on these microarrays, permitting the dissection of the signaling pathways underlying the early cellular remodeling required for regeneration. We anticipate that insights on signaling during regenerative processes in Hydra will advance our general understanding of regeneration in other multicellular species.