Project

Back to overview

Genetic Regulation of Cell and Developmental Plasticity in Hydra

English title Genetic Regulation of Cell and Developmental Plasticity in Hydra
Applicant Galliot Brigitte
Number 130337
Funding scheme Project funding (Div. I-III)
Research institution Département de Génétique et Evolution Faculté des Sciences Université de Genève
Institution of higher education University of Geneva - GE
Main discipline Embryology, Developmental Biology
Start/End 01.07.2010 - 30.09.2013
Approved amount 415'000.00
Show all

Keywords (20)

regeneration; stem cells; developmental plasticity; RNA interference; high throughput sequencing; MAPK signaling pathway; WNt signaling pathway; gene regulatory network; cellular remodeling; hydra model system; evolution; hydra; cellular remodelling; homeostasis; injury; growth control; patterning; apoptosis-induced compensatory proliferation; gene regulatory networks; signaling

Lay Summary (English)

Lead
Lay summary
A fascinating, unanswered question in biology is how some organisms respond to injury by regenerating the lost body structure, whereas other have lost this potential. Three fundamental aspects of the problem are first, how does injury trigger a growth and patterning response? Second, what are the molecular actors that support the memory to regenerate the proper missing structure? Third, how does the regenerating tissue "read" the overall size of the animal to produce an appropriately sized structure? While some molecular pathways have been implicated, a clear mechanism linking physical injury to cellular and molecular response is lacking. Here we propose to use the freshwater Hydra polyp as regeneration model organism to tackle these questions. Indeed, Hydra is a simple animal currently emerging as a powerful model system to understand how a highly dynamic homeostasis might contribute to tissue repair and regeneration. At first we assume that the mechanisms maintaining homeostasis and those underlying regeneration need to be deciphered in parallel. To identify such mechanisms, we propose complementary strategies: first we will launch high throughput transcriptome analyses in homeostasis and regeneration contexts to take a snapshot of virtually all the transcribed genes in well-defined conditions, i.e. at determined time points during regeneration, at different locations along Hydra body column. In parallel we plan to perform a functional screen to identify the different classes of phenotypes linked to alterations of the homeostasis status and/or the regenerative potential in Hydra. For that screen we will apply the RNA interference method that offers the possibility to knock-down the expression of target genes for several days. In complement we propose more focused approaches to dissect the signaling pathways linking injury to cellular responses leading to de novo patterning. After mid-gastric bisection, apoptotic cells on the head-regenerating side appear to release signaling cues and we propose to investigate the stress-induced splicing event of the CREB transcription factor, as well as the complex activation of the Wnt pathway in head-regenerating tips. Taken collectively these results on gene regulatory networks and signaling during regeneration in Hydra will allow the regeneration field to perform systematic comparisons with the mechanisms at work in other regenerative model systems (as planarians, annelids, insect larvae, echinoderms, amphibians and teleost fish) to help uncover unifying principles of tissue repair and regeneration in eumetazoans.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Publications

Publication
Injury-induced asymmetric cell death as a driving force for head regeneration in Hydra.
Galliot Brigitte (2013), Injury-induced asymmetric cell death as a driving force for head regeneration in Hydra., in Development genes and evolution, 223(1-2), 39-52.
Punctuated Emergences of Genetic and Phenotypic Innovations in Eumetazoan, Bilaterian, Euteleostome, and Hominidae Ancestors
Wenger Yvan, Galliot Brigitte (2013), Punctuated Emergences of Genetic and Phenotypic Innovations in Eumetazoan, Bilaterian, Euteleostome, and Hominidae Ancestors, in Genome Biology and Evolution, 5(10), 1949-1968.
RNAseq versus genome-predicted transcriptomes: a large population of novel transcripts identified in an Illumina-454 Hydra transcriptome.
Wenger Yvan, Galliot Brigitte (2013), RNAseq versus genome-predicted transcriptomes: a large population of novel transcripts identified in an Illumina-454 Hydra transcriptome., in BMC genomics, 14, 204-204.
Guidelines for the use and interpretation of assays for monitoring autophagy.
Klionsky Daniel J, Abdalla Fabio C, Abeliovich Hagai, Abraham Robert T, Acevedo-Arozena Abraham, Adeli Khosrow, Agholme Lotta, Agnello Maria, Agostinis Patrizia, Aguirre-Ghiso Julio A, Ahn Hyung Jun, Ait-Mohamed Ouardia, Ait-Si-Ali Slimane, Akematsu Takahiko, Akira Shizuo, Al-Younes Hesham M, Al-Zeer Munir A, Albert Matthew L, Albin Roger L, Alegre-Abarrategui Javier, Aleo Maria Francesca, Alirezaei Mehrdad, Almasan Alexandru, Almonte-Becerril Maylin, Amano Atsuo (2012), Guidelines for the use and interpretation of assays for monitoring autophagy., in Autophagy, 8(4), 445-544.
How to use Hydra as a model system to teach biology in the classroom.
Bossert Patricia, Galliot Brigitte (2012), How to use Hydra as a model system to teach biology in the classroom., in The International journal of developmental biology, 56(6-7-8), 637-652.
Hydra, a fruitful model system for 270 years.
Galliot Brigitte (2012), Hydra, a fruitful model system for 270 years., in The International journal of developmental biology, 56(6-7-8), 411-423.
Hydra, a versatile model to study the homeostatic and developmental functions of cell death.
Reiter Silke, Crescenzi Marco, Galliot Brigitte, Buzgariu Wanda (2012), Hydra, a versatile model to study the homeostatic and developmental functions of cell death., in The International journal of developmental biology, 56(6-7-8), 593-604.
The Hydra Model System.
Galliot Brigitte (2012), The Hydra Model System., in The International journal of developmental biology, 56(6-7-8), 407-409.
[Between homeostasis and development, which strategies to regenerate?].
Galliot Brigitte (2011), [Between homeostasis and development, which strategies to regenerate?]., in Biologie aujourd'hui, 205(2), 125-37.
A two-step process in the emergence of neurogenesis.
Galliot Brigitte, Quiquand Manon (2011), A two-step process in the emergence of neurogenesis., in The European journal of neuroscience, 34(6), 847-62.
Injury-induced activation of the MAPK/CREB pathway triggers apoptosis-induced compensatory proliferation in hydra head regeneration.
Chera Simona, Ghila Luiza, Wenger Yvan, Galliot Brigitte (2011), Injury-induced activation of the MAPK/CREB pathway triggers apoptosis-induced compensatory proliferation in hydra head regeneration., in Development, growth & differentiation, 53(2), 186-201.
Cell plasticity in homeostasis and regeneration.
Galliot Brigitte, Ghila Luiza (2010), Cell plasticity in homeostasis and regeneration., in Molecular reproduction and development, 77(10), 837-55.
The Hydra model: disclosing an apoptosis-driven generator of Wnt-based regeneration.
Galliot Brigitte, Chera Simona (2010), The Hydra model: disclosing an apoptosis-driven generator of Wnt-based regeneration., in Trends in cell biology, 20(9), 514-23.

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
Cell Death CSH conference Poster 08.10.2013 Cold Spring Harbor, NY, United States of America Galliot Brigitte;
Euroscicon meeting - Cell cycle analysis: the research and the technology Poster 04.10.2013 London, Great Britain and Northern Ireland Buzgariu Wanda;
13th International Congress of Invertebrate Reproduction and Development (ICIRD) Talk given at a conference 14.07.2013 Detroit, MI, United States of America Galliot Brigitte;
Embryology Course Marine Biology Laboratory Woods Hole Individual talk 03.07.2013 Woods Hole, MA, United States of America Galliot Brigitte;
Gordon Research Conferences Tissue Repair and Regeneration Talk given at a conference 16.06.2013 New London, NH, United States of America Galliot Brigitte;
Invited Seminar Institute of Cell Biology, ETH Individual talk 03.06.2013 Zürich, Switzerland Galliot Brigitte;
PhD Program in Zoology and Developmental Biology Individual talk 06.02.2013 Cambridge, Great Britain and Northern Ireland Galliot Brigitte;
The Biology of Stem Cells UPMC Stem Cell Initiative Talk given at a conference 20.12.2012 Paris, France Galliot Brigitte;
International Society of Differentiation NIMR Conference Talk given at a conference 05.11.2012 Amsterdam, Netherlands Galliot Brigitte;
EMBO conference 2012 The molecular and cellular basis of regeneration and tissue repair Talk given at a conference 02.09.2012 Oxford, Great Britain and Northern Ireland Buzgariu Wanda; Galliot Brigitte;
Arolla 2012 Cell and Developmental Systems Talk given at a conference 21.08.2012 Arolla, Switzerland Galliot Brigitte;
The S. Meryl Rose lectureship Marine Biology Laboratory, Woods Hole Individual talk 05.07.2012 Woods Hole, MA, United States of America Galliot Brigitte;
8th Annual Meeting NCCR Frontiers in Genetics (talk by Yvan Wenger) Talk given at a conference 27.06.2012 Saas-Fee, Wallis, Switzerland Galliot Brigitte;
Invited Seminar School of Molecular and Cell Biology, University of Illinois, Prof. Philipp Newmark Individual talk 05.06.2012 Urbana-Champaign, Illinois, United States of America Galliot Brigitte;
41st Annual Meeting of The American Aging Association Talk given at a conference 01.06.2012 Fort Worth, Texas, United States of America Galliot Brigitte;
Keystone Conference on Mechanisms of Whole Organ Regeneration Talk given at a conference 01.04.2012 Breckenridge, Colorado, USA, United States of America Galliot Brigitte;
Vital-IT Users Day 2012 (talk by Yvan Wenger) Talk given at a conference 30.03.2012 University of Lausanne, Switzerland, Switzerland Galliot Brigitte;
Invited Seminar at the Barshop Institute for Longevity, University of San Antonio Individual talk 30.03.2012 San Antonio, Texas, USA, United States of America Galliot Brigitte;
The 21st CDB meeting, “from Regeneration Biology to Regenerative Medicine (I)” Talk given at a conference 24.11.2011 Riken Institute, Kobe, Japan, Japan Galliot Brigitte;
Invited Seminar at the Immunology Center of Marseilles, Prof. Pierre Goldstein Individual talk 20.10.2011 Luminy-Marseille, France, France Galliot Brigitte;
Stem Cells and Regenerative Medicine Seminar Series Individual talk 05.10.2011 Medical School of Geneva, Switzerland, Switzerland Galliot Brigitte;
2nd Lübeck Regenerative Medicine Symposium Talk given at a conference 27.06.2011 Lübeck, Germany Galliot Brigitte;
Gordon Research Conference Developmental Biology Talk given at a conference 19.06.2011 Andover, NH, United States of America Galliot Brigitte;
Gordon Research Conference Tissue Repair and Regeneration Talk given at a conference 05.06.2011 New London, NH, United States of America Galliot Brigitte;
Evo-Devo: where are we, where do we go? Talk given at a conference 25.04.2011 Tourtour, France Galliot Brigitte;
Berkeley, Cell Developmental Biology Spring Symposium 2011 The Biology of Regeneration Talk given at a conference 22.03.2011 Berkeley, CA, United States of America Galliot Brigitte;
Jeudis midi des expositions de la BGE Individual talk 13.01.2011 Geneve, Switzerland Galliot Brigitte;
Journée Claude Bernard 2010, Société de Biologie Talk given at a conference 17.11.2010 Paris, France Galliot Brigitte;
Ve meeting of the Latin American Society of Developmental Biology (LASDB) Talk given at a conference 11.11.2010 Santa Cruz, Chile Galliot Brigitte;
14th Evolutionary Biology Meeting at Marseilles Talk given at a conference 21.09.2010 Marseille, France Galliot Brigitte;
The EMBO meeting 2010 Talk given at a conference 04.09.2010 Barcelone, Spain Galliot Brigitte;


Self-organised

Title Date Place
Presentation of the Marie Heim-Voegtlin program by Dr Tapernoux 26.03.2012 Faculty of Sciences, Geneva Switzerland, Switzerland

Knowledge transfer events

Active participation

Title Type of contribution Date Place Persons involved
Café sur les Cellules Souches 22.04.2013 Institut International de Lancy, Geneve, Switzerland Galliot Brigitte;
Few days in the lab for discovering Hydra research (maturity dissertation) 18.04.2012 Faculty of Sciences, Geneva, Switzerland Buzgariu Wanda;
La Science appelle les jeunes 12.03.2012 Faculty of Sciences, Geneva, Switzerland Buzgariu Wanda;
Café Scientifique sur les Cellules Souches 17.01.2012 Collège Claparède, Genève, Switzerland Galliot Brigitte;


Communication with the public

Communication Title Media Place Year
Talks/events/exhibitions La quête de la longévité. Sarah Lainé International 2013
Talks/events/exhibitions Excellentes café workshop (Unige) Western Switzerland 2012

Awards

Title Year
No award to mention 2012

Associated projects

Number Title Start Funding scheme
149630 Regulation of stem cell plasticity in Hydra 01.10.2013 Project funding (Div. I-III)
116784 Genetic regulation of developmental plasticity and neurogenesis in cnidarians 01.07.2007 Project funding (Div. I-III)

Abstract

The freshwater polyp hydra is a relatively simple animal currently emerging as a potent model system to study how cell plasticity regulates regeneration. Following mid-gastric bisection, the two hydra halves immediately initiate an asymmetric process leading to the regeneration of the missing structures in less than 3 days, thus leading to the formation of two complete animals. Interestingly, the asymmetry underlying this regenerative process is emerging very rapidly at the molecular and cellular level in two regions that were identical prior to mid-gastric bisection. As an example head regeneration requires controlled apoptosis and compensatory proliferation of distinct cell populations in head-regenerating stumps, whereas foot regeneration occurs in the absence of cell death and cell proliferation. At the signaling level, the asymmetric activation of the MAPK and Wnt3 pathways supports the early head organizer activity (Chera et al., Dev Cell 2009). To identify the genetic mechanisms underlying the establishment of a polarity and the subsequent regeneration process, we launched a RNAi screen that we plan to continue with this project. As a complementary strategy, we also want to perform a high-throughput transcriptome analysis using the illumina/Solexa method. This method allows to take a snapshot of virtually all the transcribed genes in well defined conditions, i.e. here at determined time points during regeneration and at different locations along hydra’s body column. Subsequently, customs microarrays will be produced and mRNAs from regeneration mutants generated through the RNAi feeding technique will be tested on these microarrays, permitting the dissection of the signaling pathways underlying the early cellular remodeling required for regeneration. We anticipate that insights on signaling during regenerative processes in Hydra will advance our general understanding of regeneration in other multicellular species.
-