transplantation; viral infections; graft rejection; Rhinovirus; Respiratory virus; Lung transplant recipients; Tanzania; Cycstic Fibrosis
Ambrosioni J Bridevaux PO Aubert JD Soccal P Wagner G Kaiser L. (2015), Role of rhinovirus load in the upper respiratory tract and severity of symptoms in lung transplant recipients, in Journal of Clinical Virology
, 64(March 2015), 1-5.
Ambrosioni J Bridevaux PO Wagner G Mamin A Kaiser L. (2014), Epidemiology of viral respiratory infections in a tertiary care centre in the era of molecular diagnosis, Geneva, Switzerland, 2011-2012, in Clinical Microbiol. Infect.
, 9, 0578-0584.
Pierre-Olivier Bridevaux and all (2014), Incidence and outcomes of respiratory viral infections in lung transplant recipients: a prospective study, in thorax
, 69, 32-38.
Schibler Manuel, Yerly Sabine, Vieille Gaël, Docquier Mylène, Turin Lara, Kaiser Laurent, Tapparel Caroline, Zdobnov Evgeny, Kaiser Laurent (2012), Critical analysis of rhinovirus RNA load quantification by real-time reverse transcription-PCR., in Journal of clinical microbiology
, 50(9), 2868-72.
Kieninger12 Elisabeth, Singer1 Florian, Tapparel3 Caroline, Alves12 Marco P, Latzin12 Philipp, Tan4 Hui-Leng, Bossley4 Cara, Casaulta1 Carmen, Bush4 Andrew, Davies4 Jane C, Kaiser3 Laurent, Regamey12 Nicolas (2012), High rhinovirus burden in lower airways of children with cystic fibrosis., in Chest
Tapparel Caroline, Siegrist Fredy, Petty Tom J, Kaiser Laurent, Turin Lara, Kaiser Laurent, Tapparel Caroline (2012), Picornavirus and enterovirus diversity with associated human diseases., in Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in i
, 14C, 282-293.
Vu D-L, Bridevaux P-O, Aubert J-D, Soccal P M, Kaiser L (2011), Respiratory viruses in lung transplant recipients: a critical review and pooled analysis of clinical studies., in American journal of transplantation : official journal of the American Society of Transplantation an
, 11(5), 1071-8.
Tapparel Caroline, Cordey Samuel, Junier Thomas, Farinelli Laurent, Van Belle Sandra, Soccal Paola M, Aubert John-David, Zdobnov Evgeny, Kaiser Laurent (2011), Rhinovirus genome variation during chronic upper and lower respiratory tract infections., in PloS one
, 6(6), 21163-21163.
Soccal PM Aubert JD Bridevaux PO Garbino J Thomas Y Rochat T Rochat TS Meylan P Tapparel C (2010), Upper and lower respiratory tract viral infections and acute graft rejection in lung transplant recipients., in Clinical Infectious Diseases
, 51 (2)(2010 Jul 1), 163-170.
Respiratory viruses are the most frequent cause of infections in humans and can promote seriouscomplications in immunocompromised subjects or those with chronic lung diseases. In lung transplantrecipients, the graft itself is directly exposed to these viruses and thus particularly at risk of dysfunctiononce infected. In previous studies, we have described the impact of these viruses in hospitalizedsubjects and identified unexpected complications in lung transplant recipients. These observationsand a recent pilot study have established the basis of the present multidisciplinary collaborationbetween the University Hospitals of Geneva and Lausanne. The goal is to carefully assess symptomsassociated with viral infections and to evaluate whether respiratory viruses can trigger acute rejectionand bronchiolitis obliterans, the main cause of progressive graft dysfunction and death. To achievethis goal, we will use a longitudinal clinical follow-up approach by enrolling all transplant recipients inour network together with a systematic viral screening over four winter seasons. In order to avoid anyselection bias, we have planned prescheduled screening periods that will be conducted in all cohortsubjects three times per year independent of any symptoms or clinical conditions present. In addition,subjects will be screened systematically during the first three months following transplantation andduring each acute respiratory event and bronchoalveolar lavage procedure. The virological screeningwill use an updated panel of RT-PCR assays applied systematically on upper respiratory specimensand bronchoalveolar lavage fluids when available. The diagnostic assays will be redesignedspecifically for the present project using our most recent expertise and an extensive sequencecollection. The goal here is to design assays likely to detect any divergent strains or new viral variants.This project will provide not only the epidemiological pattern of respiratory viral infections, but also adescription of the associated symptoms and lung function abnormalities. In addition, the study isdesigned to specifically assess the potential causal link between viral respiratory infections and acuteor chronic graft rejections. Another major objective will be to analyze the potential pathologicalabnormalities or specific patterns linked to viral respiratory tract infections. In summary, we willaddress the very relevant question of the short- and long-term impact of these viral infections whichhas never been studied so extensively so far. Our position at the interface between molecular virologyand clinical sciences offers the unique opportunity to correlate clinical end-points with solid virologicalfindings. We have benefited from a preliminary funding that has not only allowed us to establish thebasis of this project, but also to launch the investigation. This strategy potentiates and synergizes thepresent funding request.