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Center of excellence for design and implementation of therapeutic transgene control systems

English title Center of excellence for design and implementation of therapeutic transgene control systems
Applicant Fussenegger Martin
Number 126022
Funding scheme Project funding (Div. I-III)
Research institution Computational Systems Biology Department of Biosystems, D-BSSE ETH Zürich
Institution of higher education ETH Zurich - ETHZ
Main discipline Molecular Biology
Start/End 01.08.2009 - 31.07.2012
Approved amount 537'000.00
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All Disciplines (2)

Discipline
Molecular Biology
Other disciplines of Engineering Sciences

Keywords (8)

biopharmaceutical manufacturing; electromagnetic waves; gene networks; gene regulation; gene therapy; bonus-of-excellence; synthetic biology; synthetic control circuits

Lay Summary (English)

Lead
Lay summary
you need to take this pill three times as day" is part of a standard therapeutic scenario all of us are familiar with when medical treatment is required. Administration of small molecules and protein pharmaceuticals is the the state-of-the art therapeutic intervention scheme of modern medicine tailored to treat metabolic diseases most of which originate from de-regulation of key metabolic pathways. Rather than correcting such pathologic out-of-control metabolic circuits by substituting or blocking disease targets, this research program focuses on designing synthetic 'prosthetic control circuits' which enable automatic and self-sustained correction of any metabolic de-regulation. Akin to repairing a car by replacing broken pieces, this therapeutic strategy based on synthetic biology and synthetic gene control, will replace 'broken' biochemical pathways by synthetic ones offering a once-for-all therapeutic solution to the disease. Although this 'Star Trek'-type of therapeutic concept is still in its infancy the design and implementation of therapeutic transgene control systems forms the basis for such future therapeutic opportunities.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Publications

Publication
Design and construction of synthetic gene networks in mammalian cells.
Karlsson Maria, Weber Wilfried, Fussenegger Martin (2012), Design and construction of synthetic gene networks in mammalian cells., in Methods in molecular biology (Clifton, N.J.), 813, 359-76.
Synthetic two-way communication between mammalian cells.
Bacchus William, Lang Moritz, El-Baba Marie Daoud, Weber Wilfried, Stelling Jörg, Fussenegger Martin (2012), Synthetic two-way communication between mammalian cells., in Nature biotechnology, n.n.-n.n..
A novel reporter system for bacterial and mammalian cells based on the non-ribosomal peptide indigoidine.
Müller Marius, Ausländer Simon, Ausländer David, Kemmer Christian, Fussenegger Martin (2012), A novel reporter system for bacterial and mammalian cells based on the non-ribosomal peptide indigoidine., in Metabolic engineering, 14(4), 325-35.
Programmable single-cell mammalian biocomputers.
Ausländer Simon, Ausländer David, Müller Marius, Wieland Markus, Fussenegger Martin (2012), Programmable single-cell mammalian biocomputers., in Nature, 487(7405), 123-7.
The food additive vanillic acid controls transgene expression in mammalian cells and mice.
Gitzinger Marc, Kemmer Christian, Fluri David A, El-Baba Marie Daoud, Weber Wilfried, Fussenegger Martin (2012), The food additive vanillic acid controls transgene expression in mammalian cells and mice., in Nucleic acids research, 40(5), 37-37.
Rational design of a small molecule-responsive intramer controlling transgene expression in mammalian cells.
Ausländer David, Wieland Markus, Ausländer Simon, Tigges Marcel, Fussenegger Martin (2011), Rational design of a small molecule-responsive intramer controlling transgene expression in mammalian cells., in Nucleic acids research, 39(22), 155-155.
The use of light for engineered control and reprogramming of cellular functions.
Bacchus William, Fussenegger Martin (2011), The use of light for engineered control and reprogramming of cellular functions., in Current opinion in biotechnology, 1-8.
Molecular diversity--the toolbox for synthetic gene switches and networks.
Weber Wilfried, Fussenegger Martin (2011), Molecular diversity--the toolbox for synthetic gene switches and networks., in Current opinion in chemical biology, 15(3), 414-20.
A synthetic optogenetic transcription device enhances blood-glucose homeostasis in mice.
Ye Haifeng, Daoud-El Baba Marie, Peng Ren-Wang, Fussenegger Martin (2011), A synthetic optogenetic transcription device enhances blood-glucose homeostasis in mice., in Science (New York, N.Y.), 332(6037), 1565-8.
Ectopic expression of human mTOR increases viability, robustness, cell size, proliferation, and antibody production of chinese hamster ovary cells.
Dreesen Imke A J, Fussenegger Martin (2011), Ectopic expression of human mTOR increases viability, robustness, cell size, proliferation, and antibody production of chinese hamster ovary cells., in Biotechnology and bioengineering, 108(4), 853-66.
A designer network coordinating bovine artificial insemination by ovulation-triggered release of implanted sperms.
Kemmer Christian, Fluri David Andreas, Witschi Ulrich, Passeraub Alain, Gutzwiller Andreas, Fussenegger Martin (2011), A designer network coordinating bovine artificial insemination by ovulation-triggered release of implanted sperms., in Journal of controlled release : official journal of the Controlled Release Society, 150(1), 23-9.
Ligand-dependent regulatory RNA parts for Synthetic Biology in eukaryotes.
Wieland Markus, Fussenegger Martin (2010), Ligand-dependent regulatory RNA parts for Synthetic Biology in eukaryotes., in Current opinion in biotechnology, 21(6), 760-5.
Munc18b regulates core SNARE complex assembly and constitutive exocytosis by interacting with the N-peptide and the closed-conformation C-terminus of syntaxin 3.
Peng Ren-Wang, Guetg Claudio, Abellan Eric, Fussenegger Martin (2010), Munc18b regulates core SNARE complex assembly and constitutive exocytosis by interacting with the N-peptide and the closed-conformation C-terminus of syntaxin 3., in The Biochemical journal, 431(3), 353-61.
An engineered mammalian band-pass network.
Greber David, Fussenegger Martin (2010), An engineered mammalian band-pass network., in Nucleic acids research, 38(18), 174-174.
Synthetic gene networks in mammalian cells.
Weber Wilfried, Fussenegger Martin (2010), Synthetic gene networks in mammalian cells., in Current opinion in biotechnology, 21(5), 690-6.
The NoRC complex mediates the heterochromatin formation and stability of silent rRNA genes and centromeric repeats.
Guetg Claudio, Lienemann Philipp, Sirri Valentina, Grummt Ingrid, Hernandez-Verdun Danièle, Hottiger Michael O, Fussenegger Martin, Santoro Raffaella (2010), The NoRC complex mediates the heterochromatin formation and stability of silent rRNA genes and centromeric repeats., in The EMBO journal, 29(13), 2135-46.
A synthetic low-frequency mammalian oscillator.
Tigges Marcel, Dénervaud Nicolas, Greber David, Stelling Joerg, Fussenegger Martin (2010), A synthetic low-frequency mammalian oscillator., in Nucleic acids research, 38(8), 2702-11.
Self-sufficient control of urate homeostasis in mice by a synthetic circuit.
Kemmer Christian, Gitzinger Marc, Daoud-El Baba Marie, Djonov Valentin, Stelling Jörg, Fussenegger Martin (2010), Self-sufficient control of urate homeostasis in mice by a synthetic circuit., in Nature biotechnology, 28(4), 355-60.
Heat-stable oral alga-based vaccine protects mice from Staphylococcus aureus infection.
Dreesen Imke A J, Charpin-El Hamri Ghislaine, Fussenegger Martin (2010), Heat-stable oral alga-based vaccine protects mice from Staphylococcus aureus infection., in Journal of biotechnology, 145(3), 273-80.
The vesicle-trafficking protein munc18b increases the secretory capacity of mammalian cells.
Peng Ren-Wang, Guetg Claudio, Tigges Marcel, Fussenegger Martin (2010), The vesicle-trafficking protein munc18b increases the secretory capacity of mammalian cells., in Metabolic engineering, 12(1), 18-25.
Epigenetic engineering of ribosomal RNA genes enhances protein production.
Santoro Raffaella, Lienemann Philipp, Fussenegger Martin (2009), Epigenetic engineering of ribosomal RNA genes enhances protein production., in PloS one, 4(8), 6653-6653.

Associated projects

Number Title Start Funding scheme
112549 Center of excellence for design and implementation of therapeutic transgene control systems 01.08.2006 Project funding (Div. I-III)

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