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Effect of metals uptake on Reactive Oxygen Species (ROS) generation in ichthyotoxic algae in relation to algal blooms in marine waters

English title Effect of metals uptake on Reactive Oxygen Species (ROS) generation in ichthyotoxic algae in relation to algal blooms in marine waters
Applicant Minouflet Marion
Number 123629
Funding scheme Fellowships for prospective researchers
Research institution School of Civil and Environmental Engin. University New South Wales
Institution of higher education Institution abroad - IACH
Main discipline Ecology
Start/End 01.09.2008 - 31.08.2009
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Keywords (13)

metals; aquatic photochemistry; gymnodinium catenatum; chattenolla marina; Marine phytoplankton; Dinoflagellates; Raphidophyceae; Harmful Algal Blooms (HAB); Nutrients; Iron; Copper; Zinc; Reactive Oxygen Species (ROS)

Lay Summary (English)

Lead
Lay summary
Worldwide, health authorities regularly issue warnings against fishing, swimming or collecting seafood in estuarine and coastal waters because of the presence of Harmful Algal Blooms (HAB). The purpose of the present study was to elucidate the relationship between the replete and deplete nutrient supply of selected Australian microalgae and their toxicity to fish through the production of Reactive Oxygen Species (ROS). Batch cultures of Chattonella marina (C.m.), Gymnodinium catenatum (G.c.) and Karlodinium veneficum (K.v.) were grown at different media conditions: seawater-based medium GSe, artificial medium Aquil with different metal concentrations (Fe, Cu, Zn, Cd), natural estuarine and coastal waters from Sydney. In general, algae grew much better in GSe than in Aquil. C.m. and K.v. growth rates and maximum fluorescence in GSe were twice that in Aquil. Regarding the effects of toxic metals in Aquil, at high Cu and Zn concentrations, growth was stimulated rather than inhibited. However at high concentrations of Cd, C.m. and G.c cultures were not viable, contrary to K.v.. Moreover no algal growth was observed in any incubation in local natural waters without nutrient supplementation by nitrate and phosphate. Cultures of K.v. and G.c. produced only low H2O2 concentrations, contrary to C.m.. At high C.m. cell density in GSe, observed H2O2 concentrations reached 2.5 µM. Finally, an assay for the assessment of toxicity of the algae to gill cell tissue was developed.
Direct link to Lay Summary Last update: 21.02.2013

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