Project

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Advances in Nanoparticle Engineering with a focus on Stability, Surface, and Particle-Cell Interaction

English title Advances in Nanoparticle Engineering with a focus on Stability, Surface, and Particle-Cell Interaction
Applicant Petri-Fink Alke
Number 123373
Funding scheme SNSF Professorships
Research institution Département de Chimie Université de Fribourg
Institution of higher education University of Fribourg - FR
Main discipline Inorganic Chemistry
Start/End 01.10.2009 - 30.09.2013
Approved amount 1'407'369.00
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Keywords (6)

Nanoparticles; Interactions; Surface; Stability; Living matter; Colloidal stabili

Lay Summary (English)

Lead
Lay summary
The proposed project describes many aspects ranging from model particle synthesis, colloidal properties investigations and protein profiling in environments of varying complexity, to kinetic adsorption studies and possible future applications. Nanoparticles for biological and/or medical applications are part of the high risk, high payoff global nanotechnology phenomenon. However, the process of converting basic research in nanomedicine into commercially viable products is likely to be long and difficult. Not only do they have to be approved by the regulatory agencies, but also their manufacturing may require significant technological and cultural adjustments on the part of the producers. There is now a slowly growing awareness that our understanding of the interaction of nanoscale objects with living matter, even at the level of single cells, has not kept pace with the explosive development of nanoscience in the past. Within the community, it is now well accepted that material surfaces are modified by the adsorption of biomolecules such as proteins and there is some consensus that cellular responses to materials in a biological medium reflect the adsorbed biomolecule layer, rather than the material itself. However, the importance of the adsorbed protein layer in mediating interactions with living systems has been slower to emerge in the case of nanoparticle-protein interactions and studies slowly start to appear.The project demonstrates that the challenge is ultimately to determine the final surface of a particle in a complex biological environment. In the future it seems that nanoparticles will have to be classified in part by the manner in which they interact with proteins, an approach that has so far not been undertaken by nanoscientists. The need to understand this new surface is gradually accepted and constitutes a serious, maybe even the most serious, limitation in the field. The project is divided in three working groups, dealing with the advanced synthesis and characterization of model particles and the general issue of biological "reproducibility", the behavior of such particles in biological environments, and more profound studies including kinetic studies and future applications to use such particles as probing devices.The obtained results will put us in the position to predict the behavior of the particle. This will will have an impact on the application of nanoparticles in medicine and the relative health and environmental risk assessment of manufactured nanoparticles, both serious bottlenecks in current nanotecnology.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Publications

Publication
Bioavailability of silver nanoparticles and ions: from a chemical and biochemical perspective
Behr R, Sigg L, Clift Martin J D, Herzog F, Minghetti M, Johnston B, Petri-Fink Alke, Rothen-Rutishauser Barbara M (2013), Bioavailability of silver nanoparticles and ions: from a chemical and biochemical perspective, in J. R. Soc. Interface, 10(87), 20130396-20130396.
Bis-TEGylated Poly( p-benzamide)s: Combining Organosolubility with Shape Persistence
Schulze Maren, Michen Benjamin, Petri-Fink Alke, Kilbinger Andreas F M (2013), Bis-TEGylated Poly( p-benzamide)s: Combining Organosolubility with Shape Persistence, in Macromolecules, 46(14), 5520-5530.
Exposure of silver-nanoparticles and silver-ions to lung cells in vitro at the air-liquid interface.
Herzog Fabian, Clift Martin J D, Piccapietra Flavio, Behra Renata, Schmid Otmar, Petri-Fink Alke, Rothen-Rutishauser Barbara M (2013), Exposure of silver-nanoparticles and silver-ions to lung cells in vitro at the air-liquid interface., in Particle and Fibre Toxicology, 10(1), 11-11.
Gold nanorods: controlling their surface chemistry and complete detoxification by a two-step place exchange.
Kinnear Calum, Dietsch Herv{é}, Clift Martin J D, Endes Carola, Rothen-Rutishauser Barbara M, Petri-Fink Alke (2013), Gold nanorods: controlling their surface chemistry and complete detoxification by a two-step place exchange., in Angewandte Chemie International Edition, 52(7), 1934-1938.
Influence of Serum Supplemented Cell Culture Medium on Colloidal Stability of Polymer Coated Iron Oxide and Polystyrene Nanoparticles With Impact on Cell Interactions In Vitro
Hirsch Vera, Salaklang Jatuporn, Rothen-Rutishauser Barbara M, Petri-Fink Alke (2013), Influence of Serum Supplemented Cell Culture Medium on Colloidal Stability of Polymer Coated Iron Oxide and Polystyrene Nanoparticles With Impact on Cell Interactions In Vitro, in IEEE Transactions on Magnetics, 49(1), 402.
Nanomaterials and the human lung: what is known and what must be deciphered to realise their potential advantages?
Jud Corinne, Clift Martin J D, Petri-Fink Alke, Rothen-Rutishauser Barbara M (2013), Nanomaterials and the human lung: what is known and what must be deciphered to realise their potential advantages?, in Swiss Medical Weekly, 143, 13758-13758.
Quantification of gold nanoparticle cell uptake under controlled biological conditions and adequate resolution
Rothen-Rutishauser Barbara M, Kuhn Dagmar A, Ali Zulqurnain, Gasser Michael, Amin Faheem, Parak Wolfang J, Vanhecke Dimitri, Petri-Fink Alke, Gehr Peter, Brandenberger Christina (2013), Quantification of gold nanoparticle cell uptake under controlled biological conditions and adequate resolution, in Nanomedicine, 13.
Size-dependent Uptake of Particles by Pulmonary APC Populations and Trafficking to Regional Lymph Nodes.
Blank Fabian, Stumbles Philip A, Seydoux Emilie, Holt Patrick G, Petri-Fink Alke, Rothen-Rutishauser Barbara M, Strickland Deborah H, Von Garnier Christophe (2013), Size-dependent Uptake of Particles by Pulmonary APC Populations and Trafficking to Regional Lymph Nodes., in American Journal of Respiratory Cell and Molecular Biology, 67.
Spatial SPION Localization in Liposome Membranes
Bonnaud C{é}cile, Vanhecke Dimitri, Demurtas Davide, Rothen-Rutishauser Barbara M, Petri-Fink Alke (2013), Spatial SPION Localization in Liposome Membranes, in IEEE Transactions on Magnetics, 49(1), 166-171.
Surface charge of polymer coated SPIONs influences the serum protein adsorption, colloidal stability and subsequent cell interaction in vitro.
Hirsch Vera, Kinnear Calum, Moniatte Marc, Rothen-Rutishauser Barbara M, Clift Martin J D, Petri-Fink Alke (2013), Surface charge of polymer coated SPIONs influences the serum protein adsorption, colloidal stability and subsequent cell interaction in vitro., in Nanoscale, 5(9), 3723-3732.
Amino covalent binding approach on iron oxide nanoparticle surface: Toward biological applications
Griffete N{é}b{é}wia, Clift Martin J D, Lamouri Aazdine, Digigow Reinaldo G, Mihut Adriana M, Petri-Fink Alke, Rothen-Rutishauser Barbara M, Dietsch Herv{é} (2012), Amino covalent binding approach on iron oxide nanoparticle surface: Toward biological applications, in Colloids and Surfaces A: Physicochemical and Engineering Aspects, 415(0), 98-104.
Assessing the in vitro and in vivo toxicity of superparamagnetic iron oxide nanoparticles.
Mahmoudi Morteza, Hofmann Heinrich, Rothen-Rutishauser Barbara M, Petri-Fink Alke (2012), Assessing the in vitro and in vivo toxicity of superparamagnetic iron oxide nanoparticles., in Chemical Reviews, 112(4), 2323-2338.
Improved dynamic response assessment for intra-articular injected iron oxide nanoparticles
Crowe Lindsay A, Tobalem F, Gramoun A, Delattre B M A, Grosdemange Kerstin, Salaklang Jatuporn, Redjem Anthony, Petri-Fink Alke, Hofmann Heinrich, Vall{é}e Jean-Paul (2012), Improved dynamic response assessment for intra-articular injected iron oxide nanoparticles, in Magnetic Resonance in Medicine, 68(5), 1544-1552.
Nanoparticles and cells: an interdisciplinary approach.
Petri-Fink Alke, Rothen-Rutishauser Barbara M (2012), Nanoparticles and cells: an interdisciplinary approach., in Chimia, 66(3), 104-109.
Assessing the in vitro and in vivo toxicity of superparamagnetic iron oxide nanoparticles
Mahmoudi M., Hofmann H, Rothen-Rutishauser B., Petri-Fink A (2011), Assessing the in vitro and in vivo toxicity of superparamagnetic iron oxide nanoparticles, in Chemical Reviews, 112(4), 2323-2338.
Biomedical nanoparticles modulate specific CD4+ T cell stimulation by inhibition of antigen processing in dendritic cells.
Blank Fabian, Gerber Peter, Rothen-Rutishauser Barbara M, Sakulkhu Usawadee, Salaklang Jatuporn, De Peyer Karin, Gehr Peter, Nicod Laurent P, Hofmann Heinrich, Geiser Thomas, Petri-Fink Alke, Von Garnier Christophe (2011), Biomedical nanoparticles modulate specific CD4+ T cell stimulation by inhibition of antigen processing in dendritic cells., in Nanotoxicology, 5(4), 606-621.
Improved dynamic response assessment for intra-articular injected iron oxide nanoparticles
Crowe L.A., Tobalem F., Gramoun A., Delattre B.M.A., Grosdemange K, Salaklang J, Redjem A., Petri-Fink A, Hofmann H, Vallee J P (2011), Improved dynamic response assessment for intra-articular injected iron oxide nanoparticles, in Magnetic Resonance in Medicine, 68(5), 1544-1552.
Laser Scanning Microscopy Combined with Image Restoration to Analyse and Quantify the Intracellular Localisation of Fluorescently Labelled Nanoparticles
Rothen-Rutishauser Barbara M, Blank Fabian, Petri-Fink Alke, Clift Martin J D, Geiser Thomas, Von Garnier Christophe (2011), Laser Scanning Microscopy Combined with Image Restoration to Analyse and Quantify the Intracellular Localisation of Fluorescently Labelled Nanoparticles, in G.I.T. Imaging {&} Microscopy, 5.
Magnetic and in vitro heating properties of implants formed in situ from injectable formulations and containing superparamagnetic iron oxide nanoparticles (SPIONs) embedded in silica microparticles for magnetically induced local hyperthermia
Le Renard Pol-Edern, Lortz R, Senatore C, Rapin J P, Buchegger Franz, Petri-Fink Alke, Hofmann Heinrich, Doelker Eric, Jordan Olivier (2011), Magnetic and in vitro heating properties of implants formed in situ from injectable formulations and containing superparamagnetic iron oxide nanoparticles (SPIONs) embedded in silica microparticles for magnetically induced local hyperthermia, in Journal of Magnetism and Magnetic Materials, 323(8), 1054-1063.
Nanoparticles and cells: An interdisciplinary approach
Petri-Fink A, Rothen-Rutishauser B. (2011), Nanoparticles and cells: An interdisciplinary approach, in Chimia, 66(3), 104-109.
Polymer-coated nanoparticles alter dendritic cell-dependent T cell stimulation
Blank Fabian, Gerber Peter, Rothen-Rutishauser Barbara, Salaklang Jatuporn, Sakulkhu Usawadee, de Peyer Karin, Gehr Peter, Nicod Laurent, Hofmann Heinrich, Geiser Thomas, Petri-Fink Alke*, von Garnier Christophe* (2011), Polymer-coated nanoparticles alter dendritic cell-dependent T cell stimulation, in Nanotoxicology, 606-621.
Elegant synthesis strategies using a new magnetic bed reactor: Monoclonal mouse anti-CD11b derivatized superparamagnetic iron oxide nanoparticles
Salaklang Jatuporn, Petri-Fink Alke, Häfeli Urs, Schütt Wolfgang, Zborowski Maciej (2010), Elegant synthesis strategies using a new magnetic bed reactor: Monoclonal mouse anti-CD11b derivatized superparamagnetic iron oxide nanoparticles, in AIP.
Imaging monocytes with iron oxide nanoparticles targeted towards the monocyte integrin MAC-1 (CD11b/CD18) does not result in improved atherosclerotic plaque detection by in vivo MRI
von zur Mühlen C, Petri-Fink Alke, Salaklang Jatuporn, Paul D, Neudorfer I, Berti V, Merkle A, Peter K, Bode C, Von Elverfeldt D (2010), Imaging monocytes with iron oxide nanoparticles targeted towards the monocyte integrin MAC-1 (CD11b/CD18) does not result in improved atherosclerotic plaque detection by in vivo MRI, in Contrast Media Mol. Imaging, 5(5), 268-275.
Management of nanomaterials safety in research environment
Groso Amela, Petri-Fink Alke, Magrez Arnaud, Riediker Michael, Meyer Thierry (2010), Management of nanomaterials safety in research environment, in Particle and Fibre Toxicology, 40-44.
Management of nanomaterials safety in research environment.
Groso Amela, Petri-Fink Alke, Magrez Arnaud, Riediker Michael, Meyer Thierry (2010), Management of nanomaterials safety in research environment., in Particle and Fibre Toxicology, 7, 40-40.
Monoclonal Mouse anti- CD11b derivatized Superparamagnetic Iron Oxide nanoparticles in a new magnetic bed-reactor for the integrin cell surface receptor MAC-1
Salaklang Jatuporn, Petri-Fink Alke (2010), Monoclonal Mouse anti- CD11b derivatized Superparamagnetic Iron Oxide nanoparticles in a new magnetic bed-reactor for the integrin cell surface receptor MAC-1, in AIP Conference Proceedings, American Institute of Physics, USA.
A comparative study of different in vitro lung cell culture systems to assess the most beneficial tool for screening the potential adverse effects of carbon nanotubes
Clift Martin J D, Endes Carola, Vanhecke Dimitri, Wick Peter, Gehr Peter, Schins Roel P F, Petri-Fink Alke, Rothen-Rutishauser Barbara M, A comparative study of different in vitro lung cell culture systems to assess the most beneficial tool for screening the potential adverse effects of carbon nanotubes, in Toxicological Sciences.
Bis-TEGylated Poly(p‐benzamide)s: Combining Organosolubility with Shape Persistence
Schulze M, Michen B, Fink A, Kilbinger AFM, Bis-TEGylated Poly(p‐benzamide)s: Combining Organosolubility with Shape Persistence, in Macromolecules, 46(14), 5520.
Can the Ames test provide an insight into nano-object mutagenicity? Investigating the interaction between nano-objects and bacteria.
Clift Martin J D, Raemy David O, Endes Carola, Ali Zulqurnain, Lehmann Andrea D, Brandenberger Christina, Petri-Fink Alke, Wick Peter, Parak Wolfgang J, Gehr Peter, Schins Roel P F, Rothen-Rutishauser Barbara M, Can the Ames test provide an insight into nano-object mutagenicity? Investigating the interaction between nano-objects and bacteria., in Nanotoxicology.

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
SAOG - Swiss Working Group for Surface and Interface Science Talk given at a conference 25.01.2013 Fribourg, Switzerland Michen Benjamin;
Swiss Chemical Society Fall Meeting Zurich Talk given at a conference 13.09.2012 Zuerich, Switzerland Digigow Reinaldo;
Minneapolis, USA (9th International Conference on the Scientific and Clinical Applications of Magnetic Carriers) Talk given at a conference 22.05.2012 Minneapolis, United States of America Bonnaud Cécile;
Nanax5 Poster 07.05.2012 Fuengirola, Spain Kinnear Calum;
DFG SP 1313 Annual Meeting Talk given at a conference 07.03.2012 Fulda, Germany Hirsch Vera;
Invited presentation Individual talk 15.11.2010 NANOTEC, Bangkok, Thailand Petri-Fink Alke;
8th International Conference on the Scientific and Clinical Applications of Magnetic Carriers Talk given at a conference 25.05.2010 Rostock, Germany Salaklang Jatuporn;


Knowledge transfer events

Active participation

Title Type of contribution Date Place Persons involved
L’association de formation continue des laborants du canton de Fribourg 16.01.2013 Fribourg, Switzerland Petri-Fink Alke;
Société Fribourgeoise des Sciences naturelles 10.03.2011 Fribourg, Switzerland Petri-Fink Alke;
Tag der Forschung (SNF) 01.12.2010 Fribourg, Switzerland Petri-Fink Alke;


Communication with the public

Communication Title Media Place Year
Media relations: print media, online media "Was sie schon immer über Nano wissen wollten" International 2013

Associated projects

Number Title Start Funding scheme
128729 Nano-Optical processing and characterization of complex media for materials research and life sciences 01.01.2010 R'EQUIP
138365 Realistic exposure scenarios to study nanoparticle-lung cell interactions 01.01.2012 Project funding (Div. I-III)
150737 Nanoparticles: Surface, protein & cell interaction 01.10.2013 SNSF Professorships
139147 R'Equip grant proposal for a MALDI ToF mass spectrometer 01.04.2012 R'EQUIP

Abstract

The proposed project describes many aspects ranging from model particle synthesis, colloidal properties investigations and protein profiling in environments of varying complexity, to kinetic adsorption studies and possible future applications. The project demonstrates that the challenge is ultimately to determine the final surface of a particle in a complex biological environment. In the future it seems that nanoparticles will have to be classified in part by the manner in which they interact with proteins, an approach that has so far not been undertaken by nanoscientists. The need to understand this new surface is gradually accepted and constitutes a serious, maybe even the most serious, limitation in the field. Here, I describe my vision how to address the described challenges. The project is divided in three working groups, dealing with the advanced synthesis and characterization of model particles and the general issue of biological “reproducibility” (WP 1), the behavior of such particles in biological environments (including the stability, size and internalization of such particles by cells, the elucidation of adsorption/surface interaction of mostly proteins with such particles, and intracellular targeting possibility) (WP2), and more profound studies including kinetic studies and future applications to use such particles as probing devices (WP3).
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