microtubule; microtubule-associated-proteins; microtubule plus end tracking proteins (+TIPs); structure-function relationship; protein-protein interactions; x-ray crystography; biochemistry; biophysics; cell biology
Buey Rubén M, Mohan Renu, Leslie Kris, Walzthoeni Thomas, Missimer John H, Menzel Andreas, Bjelic Saša, Bargsten Katja, Grigoriev Ilya, Smal Ihor, Meijering Erik, Aebersold Ruedi, Akhmanova Anna, Steinmetz Michel O (2011), Insights into EB1 structure and the role of its C-terminal domain for discriminating microtubule tips from the lattice., in Molecular biology of the cell
, 22(16), 2912-23.
Akhmanova Anna, Steinmetz Michel O (2011), Microtubule end binding: EBs sense the guanine nucleotide state., in Current biology : CB
, 21(8), 283-285.
van der Vaart Babet, Manatschal Cristina, Grigoriev Ilya, Olieric Vincent, Gouveia Susana Montenegro, Bjelic Sasa, Demmers Jeroen, Vorobjev Ivan, Hoogenraad Casper C, Steinmetz Michel O, Akhmanova Anna (2011), SLAIN2 links microtubule plus end-tracking proteins and controls microtubule growth in interphase., in The Journal of cell biology
, 193(6), 1083-99.
Montenegro Gouveia Susana, Leslie Kris, Kapitein Lukas C, Buey Rubén M, Grigoriev Ilya, Wagenbach Michael, Smal Ihor, Meijering Erik, Hoogenraad Casper C, Wordeman Linda, Steinmetz Michel O, Akhmanova Anna (2010), In vitro reconstitution of the functional interplay between MCAK and EB3 at microtubule plus ends., in Current biology : CB
, 20(19), 1717-22.
Akhmanova Anna, Steinmetz Michel O (2010), Microtubule +TIPs at a glance., in Journal of cell science
, 123(Pt 20), 3415-9.
De Groot Christian O, Jelesarov Ilian, Damberger Fred F, Bjelić Sasa, Schärer Martin A, Bhavesh Neel S, Grigoriev Ilia, Buey Ruben M, Wüthrich Kurt, Capitani Guido, Akhmanova Anna, Steinmetz Michel O (2010), Molecular insights into mammalian end-binding protein heterodimerization., in The Journal of biological chemistry
, 285(8), 5802-14.
Honnappa Srinivas, Gouveia Susana Montenegro, Weisbrich Anke, Damberger Fred F, Bhavesh Neel S, Jawhari Hatim, Grigoriev Ilya, van Rijssel Frederik J A, Buey Ruben M, Lawera Aleksandra, Jelesarov Ilian, Winkler Fritz K, Wüthrich Kurt, Akhmanova Anna, Steinmetz Michel O (2009), An EB1-binding motif acts as a microtubule tip localization signal., in Cell
, 138(2), 366-76.
Steinmetz Michel O, Akhmanova Anna (2009), Capturing protein tails by CAP-Gly domains., in Trends in biochemical sciences
, 33(11), 535-545.
Komarova Yulia, De Groot Christian O, Grigoriev Ilya, Gouveia Susana Montenegro, Munteanu E Laura, Schober Joseph M, Honnappa Srinivas, Buey Rubén M, Hoogenraad Casper C, Dogterom Marileen, Borisy Gary G, Steinmetz Michel O, Akhmanova Anna (2009), Mammalian end binding proteins control persistent microtubule growth., in The Journal of cell biology
, 184(5), 691-706.
Manna Tapas, Thrower Douglas A, Honnappa Srinivas, Steinmetz Michel O, Wilson Leslie (2009), Regulation of microtubule dynamic instability in vitro by differentially phosphorylated stathmin., in The Journal of biological chemistry
, 284(23), 15640-9.
Dinkel Holger, Michael Sushama, Weatheritt Robert J, Davey Norman E, Van Roey Kim, Altenberg Brigitte, Toedt Grischa, Uyar Bora, Seiler Markus, Budd Aidan, Jödicke Lisa, Dammert Marcel A, Schroeter Christian, Hammer Maria, Schmidt Tobias, Jehl Peter, McGuigan Caroline, Dymecka Magdalena, Chica Claudia, Luck Katja, Via Allegra, Chatr-Aryamontri Andrew, Haslam Niall, Grebnev Gleb, Edwards Richard J, ELM--the database of eukaryotic linear motifs., in Nucleic acids research
Bjelić Saša, De Groot Christian O, Schärer Martin A, Jaussi Rolf, Bargsten Katja, Salzmann Mara, Frey Daniel, Capitani Guido, Kammerer Richard A, Steinmetz Michel O, Interaction of mammalian end binding proteins with CAP-Gly domains of CLIP-170 and p150(glued)., in Journal of structural biology
Microtubule plus end tracking protein (+TIP) networks play a pivotal role in all microtubule-based cellular processes including chromosome segregation, vesicle and organelle transport, delivery of proteins to the cell periphery, establishment and maintenance of cell polarity, and cell migration. Although many studies have revealed the central components forming +TIP networks, the detailed nature, specificity, and regulation modes of their dynamic interactions are largely unknown. As a consequence, the molecular mechanisms underlying the phenomenon of microtubule plus end tracking and microtubule end capturing of cellular structures by the +TIP machinery are poorly understood. In this project, we propose to investigate key factors that define and regulate the +TIP interactome with the aim to provide detailed quantitative, structural and functional information for understanding +TIP network behavior at the system level. To reach this goal we will use an integrated experimental approach encompassing cell biological, biochemical and biophysical methods. We expect that the results of the proposed work will afford a comprehensive, molecular view of a well-defined, biologically and medically important dynamic protein network. We further anticipate that the planned studies will deliver a firm conceptual and experimental basis for extending the research towards assessing the next level of complexity: the mechanisms by which the mammalian +TIP machinery targets microtubule ends to specific cellular structures such as membrane compartments, the cell cortex and kinetochores.