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Effects of UV filters in aquatic organisms on endocrine fuction and on gene expression using microarrays

English title Effects of UV filters in aquatic organisms on endocrine fuction and on gene expression using microarrays
Applicant Fent Karl
Number 121829
Funding scheme Project funding (Div. I-III)
Research institution University of Applied Sciences Northwestern Switzerland (FHNW) Institute for Ecopreneurship
Institution of higher education ETH Zurich - ETHZ
Main discipline Endocrinology
Start/End 01.02.2009 - 30.09.2012
Approved amount 288'000.00
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All Disciplines (3)

Discipline
Endocrinology
Environmental Research
Ecology

Keywords (13)

UV filters; effects on fish; reproductive effects; microarrays; sex hormone system; endocrine disrupters; ecotoxicology; aquatic toxicology; hormonal effects on fish; gene expression analysis in fish; environmental effects of cosmetics; UV-filters; glucocorticoids

Lay Summary (English)

Lead
Lay summary
LeadThis project is aimed at understanding the ecotoxicological effects of UV filters and elucidating the modes of action in fish. It contributes to the hazard and risk assessment of these largely used personal care products for aquatic ecosystems.BackgroundUV absorbing organic chemicals (UV filters) are increasingly used in sunscreens, personal care products and in the protection of materials against UV irradiation. In the aquatic environment and in fish widespread contamination was documented. We have shown that a considerable number of UV filters have hormonal activities in vitro, and some of them have estrogenic activity in fish. In fathead minnows, estrogenic 3-benzylidene camphor and benzophenone-2 caused feminization of male fish, alteration of gonads, and decrease in fertility and reproduction. We also showed that UV filter mixtures act mainly synergistically in vitro, and additive in vivo. Besides effects on the sex hormone system, inhibition of enzymes involved in the thyroid and glucocorticoid system have been demonstrated in mammals. At present it is unknown, what kind of genes or gene clusters related to these hormone systems and which additional physiological functions are affected by UV filters in fish. AimsThe aim of the project is to deepen our understanding and further investigate the ecotoxicological effects of UV filters. We will focus in more detail on molecular, biochemical and reproductive effects of UV filters in zebrafish, a species not yet studied in that respect, and of which microarrays offer major advantages for global gene expression analysis. We aim at following by means of experimental fish exposure analysis: 1.Effects of selected UV filters on fertility and reproduction of zebrafish (Danio rerio) will be studied. 2.Effects of UV filters on the activity of glucocorticoid receptor and steroid hormone metabolizing enzymes of zebrafish will be analysed.3.Effects selected UV filters on global gene expression in zebrafish using microarrays will be investigated to assess and compare the effect pathways on biological functions based on the pattern of altered gene expressions in different tissues. Altered genes and gene clusters will be further analysed to elucidate the modes of action of UV filters.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Publications

Publication
Effects of the UV filter benzophenone-3 (oxybenzone) at low concentrations in zebrafish (Danio rerio)
Blüthgen Nancy, Zucchi Sara, Fent Karl (2012), Effects of the UV filter benzophenone-3 (oxybenzone) at low concentrations in zebrafish (Danio rerio), in Toxicology and Applied Pharmacology, 363, 184-194.
Effects of the UV-filter2-ethyl-hexyl-4-trimethoxycinnamate on expression of genes involved in hormonal pathwaysinfathead minnows (Pimephales promelas) and link to vitellogenin induction and histology
Christen Verena, Zucchi Sara, Fent Karl (2011), Effects of the UV-filter2-ethyl-hexyl-4-trimethoxycinnamate on expression of genes involved in hormonal pathwaysinfathead minnows (Pimephales promelas) and link to vitellogenin induction and histology, in Aquatic Toxicology, 102, 167-176.
Global gene expression profile induced by the UV-filter 2-ethyl-hexyl-4-trimethoxycinnamate (EHMC) in zebrafish (Danio rerio)
Zucchi Sara, Oggier Daniela, Fent Karl (2011), Global gene expression profile induced by the UV-filter 2-ethyl-hexyl-4-trimethoxycinnamate (EHMC) in zebrafish (Danio rerio), in Environmental Pollution, 159, 3086-3096.
Progress and promises in toxicogenomics in aquatic toxicology: is technical innovation driving scientific innovation?
Fent Karl, Sumpter John (2011), Progress and promises in toxicogenomics in aquatic toxicology: is technical innovation driving scientific innovation?, in Aquatic Toxicology, 105S, 25-39.
The UV-absorber benzophenone-4 alters transcripts of genes involved in hormonal pathways in zebrafish (Danio rerio) eleuthero-embryos and adult males
Zucchi Sara, Blüthgen Nancy, Ieronimo Andrea, Fent Karl (2011), The UV-absorber benzophenone-4 alters transcripts of genes involved in hormonal pathways in zebrafish (Danio rerio) eleuthero-embryos and adult males, in Toxicology and Applied Pharmacology, 250, 137-146.

Collaboration

Group / person Country
Types of collaboration
University Montpellier France (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Universität Basel Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
6th SETAC World Congress Talk given at a conference Effects of Benzophenone UV filters in fish 19.05.2012 Berlin, Germany Büthgenl Nancy; Fent Karl;
PRIMO 16 Talk given at a conference Transcriptomal effects of UV filters and link to physiological effects 15.05.2011 Long Beach, USA, United States of America Fent Karl; Büthgenl Nancy;
Pollution-Induced Responses of Marine Organisms Talk given at a conference Effects of UV filters to fish 09.05.2010 University Bordeaux, France Büthgenl Nancy; Fent Karl;


Associated projects

Number Title Start Funding scheme
111393 Ökologische Risikoanalyse von UV-Filtern 01.03.2006 NRP 50 Endocrine Disruptors
141040 Progesterone and progestins in the aquatic environment: Effects on reproduction and transcriptomics in fish 01.01.2013 Project funding (Div. I-III)
124912 The Regulation of Glucocorticoid and Mineralocorticoid Hormone Action by 11beta-Hydroxysteroid Dehydrogenases 01.05.2009 Project funding (Div. I-III)

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