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Pseudomonas genetics: small RNAs in catabolite repression

English title Pseudomonas genetics: small RNAs in catabolite repression
Applicant Haas Dieter
Number 120365
Funding scheme Project funding
Research institution Département de Microbiologie Fondamentale Faculté de Biologie et de Médecine Université de Lausanne
Institution of higher education University of Lausanne - LA
Main discipline Experimental Microbiology
Start/End 01.10.2008 - 31.12.2010
Approved amount 300'000.00
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Keywords (9)

Pseudomonas; Small RNA; Catabolite repression; nutrient choice; posttranscriptional regulation; signal transduction; GacA; RsmA; Hfq

Lay Summary (English)

Lead
Lay summary
Bacteria of the genus Pseudomonas occur widely in nature and are metabolically versatile, owing to a large number of uptake systems and degradation pathways. These bacteria preferentially utilize certain good carbon sources before they degrade other, less palatable compounds. The mechanisms by which this nutrient choice is made are not well understood and will be investigated in this project. We postulate that small RNAs are required for this regulation and that they determine whether or not genes involved in degradation pathways are expressed at the level of mRNA translation. Specifically, we will investigate which regulatory proteins of Pseudomonas species participate in this mechanism. Regulation of translation via small RNAs is a recent, rapidly expanding area of research concerning not only bacteria, but also eukaryotic organisms. Understanding how central carbon metabolism in Pseudomonas species is regulated can be gratifying from a fundamental point of view and can also help explain how these microorganisms regulate their virulence or bioremediation properties in particular environments. It may ultimately become possible to attenuate the virulence of pathogenic Pseudomonas species and to improve the potential of non-pathogenic species to degrade toxic chemicals.Recent technical advances allow us to investigate bacterial gene expression by examining virtually all mRNAs and a large number of cellular metabolites simultaneously. We will exploit these technologies in collaboration with the Center of Integrative Genomics (University of Lausanne) and ETH-Zürich, and we will combine them with more traditional genetic and physiological approaches, which we have been using for many years.
Direct link to Lay Summary Last update: 21.02.2013

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Associated projects

Number Title Start Funding scheme
100180 Pseudomonas genetics: small regulatory RNAs 01.04.2003 Project funding

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