Project

Back to overview

Lipoproteins: Synthesis, localization and function in mycobacteria

English title Lipoproteins: Synthesis, localization and function in mycobacteria
Applicant Sander Peter
Number 120326
Funding scheme Project funding (Div. I-III)
Research institution Institut für Medizinische Mikrobiologie Universität Zürich
Institution of higher education University of Zurich - ZH
Main discipline Medical Microbiology
Start/End 01.04.2008 - 31.03.2011
Approved amount 243'750.00
Show all

Keywords (5)

tuberculosis; virulence; phagosome maturation; lipoprotein; secretion

Lay Summary (English)

Lead
Lay summary
Mycobacterium tuberculosis is still a major threat in our days claiming annually 1.7 million deaths worldwide. A better understanding of M. tuberculosis, its biology and host-pathogen interaction is required for rational approaches to combat this deadly disease and for the development of novel drugs and innovative vaccines. The research project focuses on two aspects: 1. lipoprotein synthesis, localization and function, 2. phagosome maturation arrest and intracellular survival. We will combine genetic and biochemical investigations and perform virulence assays along with cell biology and physiological studies a) to characterize genes of the lipoprotein synthesis pathway, b) to study the role of defined lipoproteins, c) to identify lipoprotein transport mechanisms and d) to investigate genes involved in phagosome maturation arrest.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Publications

Publication
Dissecting the complete lipoprotein biogenesis pathway in Streptomyces scabies
Widdick David A., Hicks Matthew G., Thompson Benjamin J., Tschumi Andreas, Chandra Govind, Sutcliffe Iain C., Bruelle Juliane K., Sander Peter, Palmer Tracy, Hutchings Matthew I. (2011), Dissecting the complete lipoprotein biogenesis pathway in Streptomyces scabies, in MOLECULAR MICROBIOLOGY, 80(5), 1395-1412.
Relief from Zmp1-Mediated Arrest of Phagosome Maturation Is Associated with Facilitated Presentation and Enhanced Immunogenicity of Mycobacterial Antigens
Johansen Pal, Fettelschoss Antonia, Amstutz Beat, Selchow Petra, Waeckerle-Men Ying, Keller Peter, Deretic Vojo, Held Leonhard, Kuendig Thomas M., Boettger Erik C., Sander Peter (2011), Relief from Zmp1-Mediated Arrest of Phagosome Maturation Is Associated with Facilitated Presentation and Enhanced Immunogenicity of Mycobacterial Antigens, in CLINICAL AND VACCINE IMMUNOLOGY, 18(6), 907-913.
Antibodies protect against intracellular bacteria by Fc receptor-mediated lysosomal targeting
Joller Nicole, Weber Stefan S., Mueller Andreas J., Spoerri Roman, Selchow Petra, Sander Peter, Hilbi Hubert, Oxenius Annette (2010), Antibodies protect against intracellular bacteria by Fc receptor-mediated lysosomal targeting, in PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 107(47), 20441-20446.
Cloning, expression and characterization of Mycobacterium tuberculosis lipoprotein LprF
Bruelle Juliane K., Grau Thomas, Tschumi Andreas, Auchli Yolanda, Burri Reto, Polsfuss Silke, Keller Peter M., Hunziker Peter, Sander Peter (2010), Cloning, expression and characterization of Mycobacterium tuberculosis lipoprotein LprF, in BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 391(1), 679-684.
Deletion of dop in Mycobacterium smegmatis abolishes pupylation of protein substrates in vivo
Imkamp Frank, Rosenberger Tobias, Striebel Frank, Keller Peter M., Amstutz Beat, Sander Peter, Weber-Ban Eilika (2010), Deletion of dop in Mycobacterium smegmatis abolishes pupylation of protein substrates in vivo, in MOLECULAR MICROBIOLOGY, 75(3), 744-754.
Dop functions as a depupylase in the prokaryotic ubiquitin-like modification pathway
Imkamp Frank, Striebel Frank, Sutter Markus, Oezcelik Dennis, Zimmermann Natalie, Sander Peter, Weber-Ban Eilika (2010), Dop functions as a depupylase in the prokaryotic ubiquitin-like modification pathway, in EMBO REPORTS, 11(10), 791-797.
Identification of Apolipoprotein N-Acyltransferase (Lnt) in Mycobacteria
Tschumi Andreas, Nai Corrado, Auchli Yolanda, Hunziker Peter, Gehrig Peter, Keller Peter, Grau Thomas, Sander Peter (2009), Identification of Apolipoprotein N-Acyltransferase (Lnt) in Mycobacteria, in JOURNAL OF BIOLOGICAL CHEMISTRY, 284(40), 27146-27156.
Involvement of CD252 (CD134L) and IL-2 in the Expression of Cytotoxic Proteins in Bacterial- or Viral-Activated Human T Cells
Walch Michael, Rampini Silvana K., Stoeckli Isabelle, Latinovie-Golic Sonja, Dumrese Claudia, Sundstrom Hanna, Vogetseder Alexander, Marino Joseph, Glauser Daniel L., van den Broek Maries, Sander Peter, Groscurth Peter, Ziegler Urs (2009), Involvement of CD252 (CD134L) and IL-2 in the Expression of Cytotoxic Proteins in Bacterial- or Viral-Activated Human T Cells, in JOURNAL OF IMMUNOLOGY, 182(12), 7569-7579.
Polyphosphates from Mycobacterium bovis- potent inhibitors of class III adenylate cyclases
Guo Ying Lan, Mayer Hermann, Vollmer Waldemar, Dittrich Dorothea, Sander Peter, Schultz Anita, Schultz Joachim E. (2009), Polyphosphates from Mycobacterium bovis- potent inhibitors of class III adenylate cyclases, in FEBS JOURNAL, 276(4), 1094-1103.
A synthetic mammalian gene circuit reveals antituberculosis compounds
Weber Wilfried, Schoenmakers Ronald, Keller Bettina, Gitzinger Marc, Grau Thomas, Baba Marie Daoud-El, Sander Peter, Fussenegger Martin (2008), A synthetic mammalian gene circuit reveals antituberculosis compounds, in PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 105(29), 9994-9998.
LspA inactivation in Mycobacterium tuberculosis results in attenuation without affecting phagosome maturation arrest
Rampini Silvana K., Selchow Petra, Keller Christine, Ehlers Stefan, Boettger Erik C., Sander Peter (2008), LspA inactivation in Mycobacterium tuberculosis results in attenuation without affecting phagosome maturation arrest, in MICROBIOLOGY-SGM, 154, 2991-3001.
Tuberculosis vaccine strain Mycobacterium bovis BCG Russia is a natural recA mutant
Keller Peter M., Boettger Erik C., Sander Peter (2008), Tuberculosis vaccine strain Mycobacterium bovis BCG Russia is a natural recA mutant, in BMC MICROBIOLOGY, 8, 120.

Collaboration

Group / person Country
Types of collaboration
Prof. Dr. V. Deretic, University of New Mexico United States of America (North America)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Prof. Dr. M. Rizzi, University of Piemonte Orientale, Novara Italy (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Prof. Dr. S. Ehlers, Research Center Borstel, Germany Germany (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Eilika Weber-Ban, Institute for Molecular Biology and Biophysics, ETH Zürich Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Urs Ziegler, Anatomisches Institut, Universität Zürich Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Martin Fussenegger, Institute for Chemical and Bio-Engineering, ETH Zürich Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Prof. Dr. G. Keri, Vichem Chem. Res. Ltd, Budapest Hungary (Europe)
- in-depth/constructive exchanges on approaches, methods or results
Prof. Dr. A. Apt, Moscow Russia (Europe)
- in-depth/constructive exchanges on approaches, methods or results

Associated projects

Number Title Start Funding scheme
135705 Mycobacteria: Lipoprotein synthesis, protein secretion and phagosome maturation arrest 01.04.2011 Project funding (Div. I-III)
68488 Mycobacterium tuberculosis: mechanisms of virulence 01.10.2002 Project funding (Div. I-III)

Abstract

Lipoproteins: Synthesis, localization and function in mycobacteriaMycobacterium tuberculosis is still a major threat in our days claiming annually 1.7 million deaths worldwide. A better understanding of M. tuberculosis, its biology and host-pathogen interaction is required for rational approaches to combat this deadly disease and for the development of novel drugs and innovative vaccines. The submitted research proposal focuses on two aspects: 1. lipoprotein synthesis, localization and function, 2. phagosome maturation arrest and intracellular survival. We will combine genetic and biochemical investigations and perform virulence assays along with cell biology and physiological studies a) to characterize genes of the lipoprotein synthesis pathway, b) to study the role of defined lipoproteins, c) to identify lipoprotein transport mechanisms and d) to investigate genes involved in phagosome maturation arrest.
-