Project

Back to overview

Blue light-mediated inactivation of endodontic pathogens

English title Blue light-mediated inactivation of endodontic pathogens
Applicant Bouillaguet Serge
Number 119938
Funding scheme Project funding (Div. I-III)
Research institution Section de Médecine Dentaire Faculté de Médecine Université de Genève
Institution of higher education University of Geneva - GE
Main discipline Dentistry
Start/End 01.09.2008 - 31.08.2012
Approved amount 210'000.00
Show all

Keywords (5)

PDT; dentistry; endodontic infection; ROS; blue light

Lay Summary (English)

Lead
Lay summary
Current evidence suggests that 30-60% of endodontic treatments fail because of residual or recurrent microbiological infection in the pulp canal system. Various disinfectants have been used to eliminate microbiological contamination but most cannot kill bacterial biofilms and some may damage resident tissues when used at high concentrations. Photodynamic therapy (PDT) is a promising treatment for a variety of oncological, cardiovascular, dermatological, ophthalmic and infectious diseases. PDT uses visible light to activate a chromophore that mediates a therapeutic effect, usually via the generation of intracellular reactive oxygen species (ROS). Based on the susceptibility of many bacteria to ROS, red-light lasers have recently been proposed to disinfect the pulp canal system during endodontic therapy. However, red-light has several inherent disadvantages for PDT, including thermal insult to resident tissues and relatively long therapeutic treatment times. In this project, we propose to use blue light-activated PDT for endodontic disinfection. Because of its higher energy per photon, blue light will generate ROS more efficiently, will not tend to elevate the temperature of resident tissues, and will require far shorter treatment times. However, the use of blue light for endodontic PDT-mediated disinfection is currently limited by a lack of appropriate blue light-absorbing chromophores. In this project we have formulated a central hypothesis that blue light can activate chromophores that kill endodontic pathogens but do not harm mammalian cells. Specifically, we will (1) identify candidate chromophores for blue light-mediated disinfection, (2) establish the efficacy of these chromophores as antimicrobial agents against endodontic pathogens, (3) demonstrate that candidate chromophores do not harm mammalian cells under conditions of antimicrobial efficacy.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Publications

Publication
NANOG priming before full reprogramming may generate germ cell tumours.
Grad I, Hibaoui Y, Jaconi M, Chicha L, Bergström-Tengzelius R, Sailani MR, Pelte MF, Dahoun S, Mitsiadis TA, Töhönen V, Bouillaguet S, Antonarakis SE, Kere J, Zucchelli M, Hovatta O, Feki A (2011), NANOG priming before full reprogramming may generate germ cell tumours., in Eur Cell Mater 2011, 22, 258-274.
Production of reactive oxygen species from photosensitizers activated with visible light sources available in dental offices.
Bouillaguet S, Wataha JC, Zapata O, Campo MA, Lange N, Schrenzel J (2010), Production of reactive oxygen species from photosensitizers activated with visible light sources available in dental offices., in Photomed Laser Surg, 28, 519-525.
Blue light-mediated inactivation of Enterococcus faecalis in vitro
Giorgio Pileggi, John C. Wataha, Myriam Girard, Iwona Grad, Jacques Schrenzel, Norbert Lange, Serge Bouillaguet, Blue light-mediated inactivation of Enterococcus faecalis in vitro, in Photodiagn PhotoTher.

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
Pan European Federation of the International Association for Dental Research 10.09.2008 London (UK)


Knowledge transfer events

Active participation

Title Type of contribution Date Place Persons involved
Congrès de l’Association Dentaire Française 2011 (ADF) 25.11.2011 Paris, France
Congrès de l’Association Dentaire Française 2010 (ADF) 26.11.2010 Paris, France
Annual meeting of the Society for Dental Science (SDS) 23.04.2010 Morges - CH
Annual meeting of the Swiss Society for Endodontology 22.01.2010 Berne


Communication with the public

Communication Title Media Place Year
Media relations: print media, online media Lumière bleue contre les infections dentaires. Horizons (SNF) n°85 German-speaking Switzerland Western Switzerland 13.06.2010
Media relations: print media, online media Une source lumineuse bleue pour prévenir les caries. Le Temps Economie&Finance Western Switzerland 12.01.2010
Media relations: print media, online media Honneur à l'Innovation Pulsations (magazine des HUG) Western Switzerland 13.11.2009

Awards

Title Year
Prix de l'Innovation- Hopitaux Universitaires de Genève 2009

Associated projects

Number Title Start Funding scheme
149962 Optimizing blue light-activated disinfection for Endodontic therapy 01.02.2014 Project funding (Div. I-III)
149962 Optimizing blue light-activated disinfection for Endodontic therapy 01.02.2014 Project funding (Div. I-III)

-