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Developing a novel MALDI-TOF MS to detect new discovered CAN-gene mutations in the circulation for early diagnosing, monitoring and determination of the prognosis of breast cancer

English title Developing a novel MALDI-TOF MS to detect new discovered CAN-gene mutations in the circulation for early diagnosing, monitoring and determination of the prognosis of breast cancer
Applicant Zhong Xiao Yan
Number 119722
Funding scheme Project funding
Research institution Labor für Pränatale Medizin und Gynäkologische Onkologie Frauenklinik / Universität Basel
Institution of higher education University of Basel - BS
Main discipline Clinical Cancer Research
Start/End 01.05.2008 - 30.04.2011
Approved amount 299'406.00
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All Disciplines (2)

Discipline
Clinical Cancer Research
Gynaecology

Keywords (5)

breast cancer; early diagnosis; prognosis; Biomarker; monitoring and determination

Lay Summary (English)

Lead
Lay summary
Breast cancer is the most common malignant disease in women and the second leading cause of cancer death in Western industrial countries. Early diagnosis and accurate identification of haematogenic metastases can improve the management of the disease and ultimately the success of treatment and patients' survival time. The detection of micrometastases is still difficult. The isolation of breast cancer cells in the circulation has been suggested as a new parameter for early diagnosis, monitoring disease progress and for optimizing therapy; however, a lack of sensitivity and specificity has been noticed, and the tedious enrichment procedures of rare tumour cells from the circulation limit their clinical use. We hypothesize that a sensitive MALDI-TOF MS (matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry) approach could detect tumour-derived specific DNA in the circulation of patients with breast cancer, which could be translated for clinical use.MALDI-TOF MS is a novel analytical tool used for measuring the molecular mass of samples. CAN-genes (candidate cancer genes) are genes that are found to be mutated at significant frequencies in cancers. The use of MALDI-TOF MS for identifying CAN-gene mutations offers newly diagnostic possibilities for cancer detection. We now intend to develop a novel MALDI-TOF MS-based method to detect newly discovered CAN-gene mutations in the circulation for early diagnosis and accurate identification of haematogenic metastases of breast cancer. Establishment of a testing system for investigating the clinical relevance of newly discovered CAN genes. We expect that CAN gene mutations could serve as potential biomarkers for facilitating the early diagnosis, monitoring and follow-up of breast cancer and hematogenic metastases.
Direct link to Lay Summary Last update: 21.02.2013

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