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Nanostrukturierte Polymere

English title Nanostructured Polymers
Applicant Meier Wolfgang
Number 117572
Funding scheme Project funding
Research institution Physikalische Chemie Departement Chemie Universität Basel
Institution of higher education University of Basel - BS
Main discipline Physical Chemistry
Start/End 01.10.2007 - 30.09.2010
Approved amount 595'125.00
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Keywords (7)

Amphiphilic block copolymers; biomimetic membranes; monolayers; block copolymer vesicles; membrane proteins; self assembly; solid supported block copolymer membranes

Lay Summary (English)

Lead
Lay summary
Amphiphilic block copolymers attract an increasing interest for the controlled preparation of self-asssembled materials with characteristic dimensions in the nanometer-size range. We are particularly interested in self-assembled block copolymer membranes due to their relationship to biological membranes. Here we plan to synthesize new polymers with photocleavable bonds that form vesicular structures in aqueous media. The vesicles can serve as nanometer-sized compartments for encapsulation. A photo-induced degradation of the vesicles can be used to trigger the release of encapsulated materials with a very high spatial and temporal control. Additionally we will synthesize polymers with hydrophilic glycopolymer-blocks that mimic the glycocalix of biological cells. We hope that the glycopolymer-blocks act as a ’mucosal’ layer on top of the block copolymer membranes thus allowing to develop air-stable, solid-supported block copolymer membranes that could potentially be used for biosensing. The robustness of block copolymer membranes and the possibility to tailor the underlying block copolymers with respect to the particular needs makes polymeric membranes a highly attractive alternative to conventional supported lipid membranes. Using end-group functionalized block copolymers we plan to develop protocols for a controlled, covalent immobilization of block copolymer membranes on solid supports. We will use such supported membranes and polymeric vesicles as models to investigate the molecular parameters responsible for insertion and functionality of membrane proteins in block copolymer membranes. As model proteins we will use a bacterial porin and NADH-ubiquinone oxidoreductase. Here it will be particularly interesting to systematically vary the chemical constitution of the polymers, the thickness of the hydrophobic part of the membranes and the length of the polymer brushes on their surface.
Block copolymer vesicles and membranes with integrated membrane proteins will be used as stable nanometer-sized compartments for detection of single enzyme activity and to perform coupled reaction sequences. Immobilization on solid surfaces will be used to get spatial and temporal control over the individual reaction steps. On the long run such nanodevices could be interesting for cofactor regeneration in (bio-) chemical reaction cascades.
Direct link to Lay Summary Last update: 21.02.2013

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Associated projects

Number Title Start Funding scheme
131884 Nanostructured Polymers 01.10.2010 Project funding
109136 Nanostrukturierte Polymere 01.10.2005 Project funding

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