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Genome structure, function, and regulation. Ultra high throughput sequencing machine

English title Genome structure, function, and regulation. Ultra high throughput sequencing machine
Applicant Hernandez Nouria
Number 117442
Funding scheme R'EQUIP
Research institution Centre Intégratif de Génomique Université de Lausanne
Institution of higher education University of Geneva - GE
Main discipline Molecular Biology
Start/End 01.07.2007 - 30.06.2008
Approved amount 436'204.00
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All Disciplines (2)

Molecular Biology

Keywords (6)

nucleic acid sequencing; gene expression; genome structure; cancer; development; plant-fungi symbiosis

Lay Summary (English)

Lay summary
The nucleic acids-DNA and RNA-are the principal informational molecules of the cell and measuring quantitative and qualitative variations of nucleic acids is the foundation of much research in molecular biology and biomedicine. One of the most versatile methods for measuring qualitative and quantitative variations in nucleic acids is DNA sequencing. Recent advances in DNA sequencing methods have resulted in the development of novel technologies that achieve levels of data generation 100- to 1000-fold greater than that of the current standardly used technologies. These technologies -- generally referred to as ultra high throughput DNA sequencing methods -- achieve this level of production by using massive parallelization and miniaturization of the sequencing reactions. As a result of these developments, not only have the costs and time required for nucleic acid sequencing been substantially reduced, but the range of uses to which they can be reasonably and readily applied has been substantially broadened.The aim of the project is to establish a regional facility that will implement and support an ultra high throughput DNA sequencing instrument and provide service to users from the University of Lausanne, the Centre Hospitalier Universitaire Vaudois, the Ecole Polytechnique Fédérale de Lausanne, the University of Geneva, and the Hôpitaux Universitaires de Genève. The project will initiate the use of the ultra high throughput DNA sequencing instrument with 4 specific studies designed to develop new applications for the technology as well as to demonstrate the broad range of possible uses. Three of the four studies will investigate the mechanisms used by normal and abnormal cells to control the utilization of genetic information. The appropriate use of this information is critical to the normal development and physiological functioning of organisms; inappropriate use of this information results in development defects and in disease. The forth study will analyze the variation in genetic information contained within genetically diverse populations of the arbuscular mycorrhizal fungi, which live in symbiosis with many plants. This symbiotic relationship is important as in many cases it imparts to the plant improved phosphorus acquisition, access to organic nitrogen sources, protection from root pathogens and improved water uptake characteristics. This study will add to our understanding of the ecological relationship between plants and these fungi and could lead to advances in crop improvement methods and sustainable agriculture.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Associated projects

Number Title Start Funding scheme
109941 Mechanisms of basal and regulated RNA polymerase III transcription in mammalian cells 01.11.2005 Project funding