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Slow dynamics by singlet-state NMR in solution and nitrogen-14 NMR in solids

English title Slow dynamics by singlet-state NMR in solution and nitrogen-14 NMR in solids
Applicant Bodenhausen Geoffrey
Number 116699
Funding scheme Project funding (Div. I-III)
Research institution Laboratoire de résonance magnétique biomoléculaire EPFL - SB - ISIC - LRMB
Institution of higher education EPF Lausanne - EPFL
Main discipline Organic Chemistry
Start/End 01.04.2007 - 31.03.2009
Approved amount 461'281.00
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Keywords (9)

Singlet-state NMR in liquids; Nitrogen-14 NMR in solids; Slow dynamic processes; nuclear magnetic resonance (NMR); enhanced polarisation; liquid state NMR; solid state NMR; long lifetimes; nitrogen-14

Lay Summary (English)

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Lay summary
The main advantage of nuclear magnetic resonance (NMR) lies in its ability to differentiate between molecules or between different sites in a molecule based on their chemical environment. Its main drawback is the limited sensitivity, typically NMR measurements needing concentrations on the order of 10-3 M in half-ml volumes for liquid-state NMR and app. 10 mg of powder for solid-state NMR. These concentrations are often hard (or expensive) to obtain for samples of interest. Moreover, biologically relevant reactions occur at much lower concentrations.The sensitivity of NMR may be enhanced if nuclear spins are coupled to unpaired electron spins, which have a much higher polarization. This can be achieved in a sample where a paramagnetic radical is mixed with the molecule of interest via the effect known as Dynamic Nuclear Polarization (DNP). We aim to apply this method to both liquid and solid-state NMR, in the latter case namely for the study spin-1 nuclei such as nitrogen-14, which is present in most biomolecules in natural abundance.The short lifetimes of spin order in comparison to the characteristic time of biological reactions are a serious barrier in the way of applications of hyperpolarized magnetic resonance to liquid state NMR. The magnetization of nuclei with low gyromagnetic ratios has low relaxation rate constants, but the sensitivity for detection of such nuclei is limited. We plan to show that long lifetimes of enhanced magnetization may be obtained on the sensitive proton spins.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

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Associated projects

Number Title Start Funding scheme
124694 Methods for enhancing sensitivity and magnetisation lifetimes in liquid- and solid-state NMR 01.04.2009 Project funding (Div. I-III)
121262 R'Equip - Amélioration de deux spectromètres RMN 9.4T par des consôles digitales 01.07.2008 R'EQUIP
107599 Résonance magnétique nucléaire (RMN) de biomolécules en phases solide et liquide 01.04.2005 Project funding (Div. I-III)
121303 Gyrotron for Dynamic Nuclear Polarisation Enhancement in Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy 01.03.2009 R'EQUIP
122708 Dynamic Nuclear Polarization Enhancement of Magic-Angle-Spinning Nuclear Magnetic Resonance Spectroscopy using gyrotrons 01.03.2009 Sinergia
124694 Methods for enhancing sensitivity and magnetisation lifetimes in liquid- and solid-state NMR 01.04.2009 Project funding (Div. I-III)

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