Project

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NMR in structural biology: protein structure determination and studies of protein-protein interactions

English title NMR in structural biology: protein structure determination and studies of protein-protein interactions
Applicant Wider Gerhard
Number 113730
Funding scheme Project funding
Research institution Institut für Molekularbiologie und Biophysik ETH Zürich
Institution of higher education ETH Zurich - ETHZ
Main discipline Biophysics
Start/End 01.01.2007 - 30.06.2010
Approved amount 260'203.00
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All Disciplines (3)

Discipline
Biophysics
Biochemistry
Molecular Biology

Keywords (6)

Structural biology; NMR spectroscopy; protein structures; FtsZ; polymerization; ubiquitin

Lay Summary (English)

Lead
Lay summary
FtsZ is the prokaryotic homolog of tubulin. This GTPase is essential for bacterial cell division and is widely conserved in bacteria. However, FtsZ is absent in mammalian cells which makes it an attractive broad-spectrum antibacterial target. The goal of the project is the characterization of structural and dynamic properties of FtsZ. In this study we want to find the structural basis for the correlation between two related aspects of the function of FtsZ, namely the GTPase activity and the polymerization process. For a better understanding of the mechanism underlying the GTPase activity, we will investigate the conformation and dynamic properties in the Apo form of FtsZ and compare it with the complex with GDP and non-hydrolyzable analogous of GTP. For the latter studies non-polymerizing variants of FtsZ will be used. The structural characterization by NMR will be further facilitated by the availability of independently folding subdomains of FtsZ. Understanding the structural basis of the functions of FtsZ will support the rational design of novel compounds that may be used as antibiotics. Based on our results Prof. Monasterio (University of Chile, Santiago) will perform in-vivo studies with variants of FtsZ which in turn will raise new questions about their structural and dynamic properties.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Associated projects

Number Title Start Funding scheme
100399 NMR in structural biology: methods development for protein structure determination 01.10.2003 Project funding
121270 Request for a 900 MHz Cryoprobe with Platform 01.09.2008 R'EQUIP
120048 Technische Entwicklunen in biomolekularer NMR Spektroskopie 01.06.2009 Project funding

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