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Broadening the scope of artificial metalloenzymes based on the biotinavidin technology

English title Broadening the scope of artificial metalloenzymes based on the biotinavidin technology
Applicant Ward Thomas R.
Number 113348
Funding scheme Project funding
Research institution Institut für Anorganische Chemie Universität Basel
Institution of higher education University of Neuchatel - NE
Main discipline Inorganic Chemistry
Start/End 01.10.2006 - 30.09.2009
Approved amount 720'115.00
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Keywords (7)

artificial metalloenzyme; second coordination sphere; enantioselective catalysis; high-throghput screening; bio-organometallic chemistry; biotin-avidin technology; high-throughput screening

Lay Summary (English)

Lead
Lay summary
Homogeneous- and enzymatic catalysis are in many respects complementary.Upon anchoring an organometallic complex into a host protein, we create artificial metalloenzymes with properties reminiscent of both kingdoms.
By exploiting the biotin (strept)avidin technology to incorporate an achiral catalyst precursor within a protein host, we have recently reported on enantioselective hydrogenation and transfer-hydrogenation reactions. A combined chemogenetic procedure allows us to rapidly optimize the selectivity of the artificial metalloenzymes (up to 96% ee).Current challenges for this funding period include:
i) The implementation of directed evolution protocols towards the optimization of artificial metalloenzymes.
ii) Enantioselective oxidation reactions (epoxidation, oxidation of sulfides and kinetic resolution of racemic alcohols).
iii) Palladium catalyzed C-C bond forming reactions (Suzuki coupling and allylic alkylation).
Direct link to Lay Summary Last update: 21.02.2013

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Associated projects

Number Title Start Funding scheme
105192 Proteins as Second Coordination Sphere Ligands in Enantioselective Catalysis 01.10.2004 Project funding
126366 Designed Evolution of Artificial Metalloenzymes Based on Streptavidin or Carbonic Anhydrase as Protein Scaffold 01.10.2009 Project funding

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