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Kinetic analysis of S.aureus biofilm regulation by using combined imaging and genome-wide approaches

English title Kinetic analysis of S.aureus biofilm regulation by using combined imaging and genome-wide approaches
Applicant Schrenzel Jacques
Number 112370
Funding scheme Project funding (Div. I-III)
Research institution Laboratoire de Recherche Génomique Service des Maladies Infectieuses Hôpital Cantonal - HUG
Institution of higher education University of Geneva - GE
Main discipline Experimental Microbiology
Start/End 01.05.2006 - 30.06.2009
Approved amount 335'000.00
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Keywords (6)

staphylococcus aureus; biofilms; host-pathogen interactions; chronic infections; foreign-body infections; mRNA messenger profiling

Lay Summary (English)

Lay summary
S. aureus is a ubiquitous gram-positive bacterium, able to exist on inanimate objects as well as in a variety of niches, such as the skin, mucous membranes, blood, or bones. During its lifetime, a single bacterium can travel from one location to another, exposing the bacteria to multiple environments. These transitions are very likely to require quick switches from a unicellular planktonic lifestyle to settle multicellular communities commonly referred to as biofilms.
In this application, we will further analyze the 38 biofilm-deficient mutants uncovered during a genetic screen and characterize in details at least three genes involved in biofilm formation. These genes were selected because of compelling evidence derived from concomitant transcriptomic and proteomic analyses, as well as from extensive literature search. To achieve these goals, we have set the following objectives :
(i) genetically validate the most promising targets by standard inactivation and complementation strategies,
(ii) characterize these three genes using different physiological and biochemical assays,
(iii) perform morphological analysis of biofilm development and maturation using confocal light scanning microscopy of fluorescently-labeled S. aureus,
(iv) correlate morphologic stages with defined patterns of gene expression, (v) use the dual fluorescent reporter system for simultaneously monitoring differential promoter activities of key biofilm-controlling genes, and
(vi) characterize the metabolic conditions of the 38 biofilm-deficient mutants by combined transcriptomic and phenotypic array experiments.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants


Associated projects

Number Title Start Funding scheme
103002 Regulation of Staphylococcus aureus biofilm development studied by genome-wide expression analysis 01.05.2004 SNSF Professorships
124717 Kinetic Analysis of S.aureus Biofilm Regulation by Using Combined Imaging and Genome-Wide Approaches 01.07.2009 Project funding (Div. I-III)
121418 Ultra High Throughput sequencing platform for functional genome analysis 01.08.2008 R'EQUIP