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Mechanism of calcium phosphate nucleation and growth mediated by proteins

English title Mechanism of calcium phosphate nucleation and growth mediated by proteins
Applicant Taubert Andreas
Number 107521
Funding scheme Project funding
Research institution Institut für Chemie Universität Potsdam
Institution of higher education University of Basel - BS
Main discipline Physical Chemistry
Start/End 01.06.2005 - 31.05.2006
Approved amount 49'266.00
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Keywords (11)

calcium phosphate; peptide surfaces; biomineralization; nucleation; crystal growth; bone formation; (bio)mineralization; microscopy; Raman spectroscopy; computer; simulation

Lay Summary (English)

Lead
Lay summary
In the recent past, CaP biomineralization research has focused on: (1) themorphogenesis of CaP in polymer gels and on polymer surfaces (mainlystudied with X-ray scattering and microscopy), (2) the interaction ofoligopeptides with CaP surfaces (studied with computational chemistry andNMR spectroscopy), and (3) the interaction of large proteins with CaP(studied with various techniques including genetic modification of theprotein structure and NMR).

The work proposed here aims at quantitatively determining the interactionof model peptides with various ions and to in detail investigate thenucleation and early crystallization stages of CaP biomineralization. Wewill use peptide surfaces derived from proteins known to interact withCaP. We will study the peptide-ion interactions, nucleation, and growthwith various techniques including the quartz crystal microbalance, Ramanmicroscopy, and atomic force microscopy. Furthermore we will simulate thenucleation and growth of CaP crystals as well as the interaction of thepeptide segments with already existing CaP surfaces. The computersimulation will provide a detailed image of nucleation, initial crystalgrowth, and peptide-CaP interactions of peptides studied in thelaboratory. The computational chemistry will thus support and enhance theexperimental data.
Direct link to Lay Summary Last update: 21.02.2013

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