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Resveratrol long-term treatment differentiates INS-1E beta-cell towards improved glucose response and insulin secretion

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Casimir Marina, Chaffard Gaelle, Maechler Pierre,
Project Glutamate pathways and metabolic stresses in energy homeostasis
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Original article (peer-reviewed)

Journal Pflügers Archiv - European Journal of Physiology
Volume (Issue) 471(2)
Page(s) 337 - 345
Title of proceedings Pflügers Archiv - European Journal of Physiology
DOI 10.1007/s00424-018-2215-z


The clonal INS-1E beta-cell line has proven to be instrumental for numerous studies investigating the mechanisms of glucose-stimulated insulin secretion. The composition of its culture medium has not changed over the years, although some compounds have been recently highlighted for their effects on tissue differentiation. The present study investigated the effects of long-term treatment of INS-1E cells with 1 μM resveratrol on glucose-stimulated insulin secretion, testing an extended glucose dose response. The data demonstrate that chronic exposure to low-dose resveratrol expands the range of the glucose dose response of INS-1E cells beyond 15 mM glucose. We also assessed whether such beneficial effects could be retained after resveratrol withdrawal from the culture medium. This was not the case as INS-1E cells deprived of resveratrol returned to the phenotype of naïve cells, i.e., exhibiting a plateau phase at 15 mM glucose. Of note, although resveratrol has antioxidant properties, it cannot substitute for β-mercaptoethanol normally present in the medium of INS-1E cells as a reducing agent. In conclusion, the addition of resveratrol as a standard component of the culture medium of INS-1E cells improves glucose-stimulated insulin secretion.