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Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Publication date 2012
Author Olarte Omar E, Licea-Rodriguez Jacob, Palero Jonathan A, Gualda Emilio J, Artigas David, Mayer Jürgen, Swoger Jim, Sharpe James, Rocha-Mendoza Israel, Rangel-Rojo Raul, Loza-Alvarez Pablo,
Project Examining the function of lymphoid organ structure during antiviral immune responses using microscopic and mesoscopic imaging
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Original article (peer-reviewed)

Journal Biomedical optics express
Volume (Issue) 3(7)
Page(s) 1492 - 505
Title of proceedings Biomedical optics express
DOI 10.1364/BOE.3.001492


We present the implementation of a combined digital scanned light-sheet microscope (DSLM) able to work in the linear and nonlinear regimes under either Gaussian or Bessel beam excitation schemes. A complete characterization of the setup is performed and a comparison of the performance of each DSLM imaging modality is presented using in vivoCaenorhabditis elegans samples. We found that the use of Bessel beam nonlinear excitation results in better image contrast over a wider field of view.