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Alternative Computational Analysis Shows No Evidence for Nucleosome Enrichment at Repetitive Sequences in Mammalian Spermatozoa.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Royo Hélène, Stadler Michael Beda, Peters Antoine Hendrik Felix Marie,
Project Establishment of chromatin states in mouse sperm and their relevance for paternal epigenetic inheritance
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Original article (peer-reviewed)

Journal Developmental cell
Volume (Issue) 37(1)
Page(s) 98 - 104
Title of proceedings Developmental cell
DOI 10.1016/j.devcel.2016.03.010


Samans et al. (2014) reported the enrichment of nucleosomes in human and bovine spermatozoa at centromere repeats and retrotransposon sequences such as LINE-1 and SINE. We demonstrate here that nucleosomal enrichments at repetitive sequences as reported result from bioinformatic analyses that make redundant use of sequencing reads that map to multiple locations in the genome. To illustrate that this computational approach is flawed, we observed comparable artificial enrichments at repetitive sequences when aligning control genomic DNA or simulated reads of uniform genome coverage. These results imply that the main conclusions of the article by Samans et al. (2014) are confounded by an inappropriate computational methodology used to analyze the primary data.