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Bioautographic screening of Xanthine Oxidase inhibition and antioxidant activities for bioprocess control of in vitro cultivated medicinal and aromatic plants of the Balkan region

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Publication date 2014
Author Wolfram E, Danova K, Könye R, Pedrussio S, Aneva L , Evstatieva , Bräm S, Meier B,
Project PhytoBalk
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Original article (peer-reviewed)

Journal Planta medica
Volume (Issue) 80
Page(s) P2B62
Title of proceedings Planta medica
DOI 10.1055/s-0034-1394939


In vitro cultivation is considered as a prospective, supplementary agriculture and season independent approach for supply of plant raw material [1]. We report here on intermediate results of research activities that aim at development of an in vitro collection of medicinal and aromatic plants from the Balkan region. Optimizations of medium composition, as well as development of callus, suspension and root cultures are being conducted in order to stimulate production of secondary metabolites with phytopharmaceutical value. Bioprocess control strategies are needed for rapid and cost effective screening for selection of highly productive in vitro lines for later scale-up in bioreactor conditions. HPTLC fingerprinting coupled with DPPH, β-carotene/linoleic acid bleaching [2], as well as bioautographic Xanthine Oxidase Inhibition [3,4] assays, were applied in order to assess secondary metabolite and active compound content simultaneously. Different in vitro lines of Balkan endemic Sideritis scardica Griseb., Pulsatilla montana ssp. balcana, essential oil bearing Artemisia alba Turra, as well as several representatives of the Hypericum genus, differing in their biosynthetic capacity for hypericines have been studied. Hypericine non-producing Hypericum calycinum L. showed superior antioxidant activity as compared with the other species. In vitro cultured S. scardica originating from wild accessions showed higher activity than from field cultivation. From all tested species, A. alba showed several inhibitory zones in the HPTLC coupled Xanthine Oxidase Inhibition screening. Further analysis of these zones is feasible by coupled HPTLC-MS. The applied bioautographic bioprocess control methods provide for rapid and efficient targeting of prospective highly producing in vitro lines for future up-scaling, especially when a large number of culture variants have to be handled. Acknowledgements: SNF No. IZEBZ0_142989 and SD-MEYS No. DO2 – 1153