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Intramembrane Cleavage of AMA1 Triggers Toxoplasma to Switch from an Invasive to a Replicative Mode

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Publication date 2011
Author Santos JM, Ferguson DJP, Blackman MJ, Soldati-Favre D,
Project Study of factors governing the invasive and replicative modes in Apicomplexa
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Original article (peer-reviewed)

Journal SCIENCE
Volume (Issue) 331(6016)
Page(s) 473 - 477
Title of proceedings SCIENCE

Open Access

URL http://archive-ouverte.unige.ch/unige:14355
Type of Open Access Repository (Green Open Access)

Abstract

Apicomplexan parasites invade host cells and immediately initiate cell division. The extracellular parasite discharges transmembrane proteins onto its surface to mediate motility and invasion. These are shed by intramembrane cleavage, a process associated with invasion but otherwise poorly understood. Functional analysis of Toxoplasma rhomboid 4, a surface intramembrane protease, by conditional overexpression of a catalytically inactive form produced a profound block in replication. This was completely rescued by expression of the cleaved cytoplasmic tail of Toxoplasma or Plasmodium apical membrane antigen 1 (AMA1). These results reveal an unexpected function for AMA1 in parasite replication and suggest that invasion proteins help to promote parasite switch from an invasive to a replicative mode.
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