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Shotgun ecotoxicoproteomics of Daphnia pulex: biochemical effects of the anticancer drug tamoxifen

Publikationsart Peer-reviewed
Publikationsform Originalbeitrag (peer-reviewed)
Publikationsdatum 2015
Autor/in BORGATTA Myriam, HERNANDEZ Céline, DECOSTERD Laurent Arthur, CHEVRE Nathalie, WARIDEL Patrice,
Projekt Ecotoxicoproteomics approach to assess the risk of anticancer drugs to aquatic fauna
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Originalbeitrag (peer-reviewed)

Zeitschrift Journal of Proteome Research
Volume (Issue) 14(1)
Seite(n) 279 - 291
Titel der Proceedings Journal of Proteome Research
DOI 10.1021/pr500916m

Abstract

Among pollutants released into the environment by human activities, residues of pharmaceuticals are an increasing matter of concern because of their potential impact on ecosystems. The aim of this study was to analyse differences of protein expression resulting from acute (2 days) and middle-term (7 days) exposure of aquatic microcrustacean Daphnia pulex to the anticancer drug tamoxifen. Using a liquid chromatography - mass spectrometry shotgun approach, about 4000 proteins could be identified, providing the largest proteomics dataset of D. pulex published up to now. Considering both time points and tested concentrations, 189 proteins showed a significant fold change. The identity of regulated proteins suggested a decrease in translation, an increase in protein degradation and changes in carbohydrate and lipid metabolism as the major effects of the drug. Besides these impacted processes, which reflect a general stress response of the organism, some other regulated proteins play a role in Daphnia reproduction. These latter results are in accordance with our previous observations of the impact of tamoxifen on D. pulex reproduction, and illustrate the potential of ecotoxicoproteomics to unravel links between xenobiotic effects at the biochemical and at the organismal level. Data are available via ProteomeXchange with identifier PXD001257.
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