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Soil environmental conditions and microbial build-up mediate the effect of plant diversity on soil nitrifying and denitrifying enzyme activities in temperate grasslands.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Publication date 2013
Author Le Roux Xavier, Schmid Bernhard, Poly Franck, Barnard Romain L, Niklaus Pascal A, Guillaumaud Nadine, Habekost Maike, Oelmann Yvonne, Philippot Laurent, Salles Joana Falcao, Schloter Michael, Steinbeiss Sibylle, Weigelt Alexandra,
Project Mechanisms underlying plant community productivity, stability and assembly (D-A-CH/LAE)
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Original article (peer-reviewed)

Journal PloS one
Volume (Issue) 8(4)
Page(s) 61069 - 61069
Title of proceedings PloS one
DOI 10.1371/journal.pone.0061069

Open Access

URL http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0061069
Type of Open Access Publisher (Gold Open Access)

Abstract

Random reductions in plant diversity can affect ecosystem functioning, but it is still unclear which components of plant diversity (species number - namely richness, presence of particular plant functional groups, or particular combinations of these) and associated biotic and abiotic drivers explain the observed relationships, particularly for soil processes. We assembled grassland communities including 1 to 16 plant species with a factorial separation of the effects of richness and functional group composition to analyze how plant diversity components influence soil nitrifying and denitrifying enzyme activities (NEA and DEA, respectively), the abundance of nitrifiers (bacterial and archaeal amoA gene number) and denitrifiers (nirK, nirS and nosZ gene number), and key soil environmental conditions. Plant diversity effects were largely due to differences in functional group composition between communities of identical richness (number of sown species), though richness also had an effect per se. NEA was positively related to the percentage of legumes in terms of sown species number, the additional effect of richness at any given legume percentage being negative. DEA was higher in plots with legumes, decreased with increasing percentage of grasses, and increased with richness. No correlation was observed between DEA and denitrifier abundance. NEA increased with the abundance of ammonia oxidizing bacteria. The effect of richness on NEA was entirely due to the build-up of nitrifying organisms, while legume effect was partly linked to modified ammonium availability and nitrifier abundance. Richness effect on DEA was entirely due to changes in soil moisture, while the effects of legumes and grasses were partly due to modified nitrate availability, which influenced the specific activity of denitrifiers. These results suggest that plant diversity-induced changes in microbial specific activity are important for facultative activities such as denitrification, whereas changes in microbial abundance play a major role for non-facultative activities such as nitrification.
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