Publication

Title

A differential concentration-dependent effect of IVIg on neutrophil functions: relevance for anti-microbial and anti-inflammatory mechanisms.

Type of publication Peer-reviewed
Form of publication Original article (peer-reviewed)
Publication date 2011
Author Casulli Sarah, Topçu Selma, Fattoum Lakhdar, von Gunten Stephan, Simon Hans-Uwe, Teillaud Jean-Luc, Bayry Jagadeesh, Kaveri Srini V, Elbim Carole
Project Role of autophagy in granulocyte survival and differentiation

Original article (peer-reviewed)

Journal PloS one
Volume (Issue) 6(10)
Page(s) 26469 - 26469
State Published
DOI 10.1371/journal.pone.0026469

Abstract

BACKGROUND Polymorphonuclear neutrophils (PMN) play a key role in host defences against invading microorganisms but can also potentiate detrimental inflammatory reactions in case of excessive or misdirected responses. Intravenous immunoglobulins (IVIg) are used to treat patients with immune deficiencies and, at higher doses, in autoimmune, allergic and systemic inflammatory disorders. METHODOLOGY/PRINCIPAL FINDINGS We used flow cytometry to examine the effects of IVIg on PMN functions and survival, using whole-blood conditions in order to avoid artifacts due to isolation procedures. IVIg at low concentrations induced PMN activation, as reflected by decreased L-selectin and increased CD11b expression at the PMN surface, oxidative burst enhancement, and prolonged cell survival. In contrast, IVIg at higher concentrations inhibited LPS-induced CD11b degranulation and oxidative burst priming, and counteracted LPS-induced PMN lifespan prolongation. CONCLUSIONS/SIGNIFICANCE IVIg appears to have differential, concentration-dependent effects on PMN, possibly supporting the use of IVIg as either an anti-microbial or an anti-inflammatory agent.

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