metabolism; Lipids droplets; chloroplast; vitamin E; Vitamin K; lipidomic; plastoglobules
Martinis Jacopo, Glauser Gaétan, Valimareanu Sergiu, Stettler Michaela, Zeeman Samuel C, Yamamoto Hiroshi, Shikanai Toshiharu, Kessler Felix (2014), ABC1K1/PGR6 kinase: a regulatory link between photosynthetic activity and chloroplast metabolism., in The Plant journal : for cell and molecular biology
, 77(2), 269-83.
Demarsy Emilie, Lakshmanan Ashok M., Kessler Felix (2014), Border control: selectivity of chloroplast protein import and regulation at the TOC-complex, in FRONTIERS IN PLANT SCIENCE
, 5, 483.
Kessler Felix, Glauser Gaetan (2014), Prenylquinone profiling in whole leaves and chloroplast subfractions., in Methods in molecular biology (Clifton, N.J.)
, 1153, 213-26.
Piller Lucia Eugeni, Glauser Gaetan, Kessler Felix, Besagni Celine (2014), Role of plastoglobules in metabolite repair in the tocopherol redox cycle, in FRONTIERS IN PLANT SCIENCE
, 5, 1-10.
Rottet Sarah, Besagni Céline, Kessler Felix (2014), The role of plastoglobules in thylakoid lipid remodeling during plant development, in Biochimica et Biophysica Acta - Bioenergetics
, 1847(9), 889-899.
Martinis Jacopo, Glauser Gaetan, Valimareanu Sergiu, Kessler Felix (2013), A Chloroplast ABC1-like Kinase Regulates Vitamin E Metabolism in Arabidopsis, in PLANT PHYSIOLOGY
, 162(2), 652-662.
Besagni Celine, Kessler Felix (2013), A mechanism implicating plastoglobules in thylakoid disassembly during senescence and nitrogen starvation, in PLANTA
, 237(2), 463-470.
Shanmugabalaji Venkatasalam, Besagni Celine, Piller Lucia Eugeni, Douet Veronique, Ruf Stephanie, Bock Ralph, Kessler Felix (2013), Dual targeting of a mature plastoglobulin/fibrillin fusion protein to chloroplast plastoglobules and thylakoids in transplastomic tobacco plants, in PLANT MOLECULAR BIOLOGY
, 81(1-2), 13-25.
Kessler Felix (2012), Chloroplast Delivery by UPS, in SCIENCE
, 338(6107), 622-623.
Lippold Felix, vom Dorp Katharina, Abraham Marion, Hoelzl Georg, Wewer Vera, Yilmaz Jenny Lindberg, Lager Ida, Montandon Cyrille, Besagni Celine, Kessler Felix, Stymne Sten, Doermann Peter (2012), Fatty Acid Phytyl Ester Synthesis in Chloroplasts of Arabidopsis, in PLANT CELL
, 24(5), 2001-2014.
Piller Lucia Eugeni, Abraham Marion, Doermann Peter, Kessler Felix, Besagni Celine (2012), Plastid lipid droplets at the crossroads of prenylquinone metabolism, in JOURNAL OF EXPERIMENTAL BOTANY
, 63(4), 1609-1618.
Sakuraba Yasuhito, Schelbert Silvia, Park So-Yon, Han Su-Hyun, Lee Byoung-Doo, Andres Celine Besagni, Kessler Felix, Hoertensteiner Stefan, Paek Nam-Chon (2012), STAY-GREEN and Chlorophyll Catabolic Enzymes Interact at Light-Harvesting Complex II for Chlorophyll Detoxification during Leaf Senescence in Arabidopsis, in PLANT CELL
, 24(2), 507-518.
Vitamins E and K are important components of nutrition and essential for human health. Vitamin E (tocopherol group) consists of closely related lipid-soluble antioxidants. They inhibit reactive oxygen species production due to lipid peroxidation. Vitamin K (phyllo-/menaquinone group) are lipid-soluble compounds that are necessary for modification of proteins implicated in blood coagulation. Together with plastoquinone, Vitamins E and K belong to the family of prenylquinone compounds. In plants, these are synthesized mostly in the chloroplast and carry important roles as lipid antioxidants (tocopherol) and/or electron carriers in photosynthesis (phylloquinone and plastoquinone), respectively.The photosynthetic light reactions in the chloroplast take place at the thylakoid membrane and this is where the prenylquinones fulfill most of their essential roles in plants. In contrast, most of the biosynthetic reactions take place in the envelope membranes. But we recently discovered that chloroplast lipid droplets called plastglobules that tightly associate with the thylakoid membrane also play an essential role in prenylquinone metabolism. Using a proteomics approach we identified about 30 different proteins coating plastoglobules. These ranged from the structural plastoglobulins, to a number of known enzymes (including the tocopherol cyclase VTE1 required for tocopherol biosynthesis) as well as uncharacterized proteins mostly predicted to be involved in lipid metabolism. We therefore tested the hypothesis that plastoglobules are indeed implicated in lipid and more specifically in prenylquinone metabolism. Using state of the art lipidomics techniques, among others, this lead to the discovery of essential metabolic roles of plastoglobules in Vitamin E (tocopherol) and Vitamin K (phylloquinone) accumulation. More important functions certainly remain to be discovered. This project has three aims and implicates a broad range of techniques:A) to systematically unravel the function of several yet uncharacterized plastoglobule proteins. These have hypothetical functions in the regulation of prenylquinone synthesis and their trafficking between plastoglobules and the thylakoid membrane.B) to adress the hypothesis that plastoglobules are implicated in the biogenesis of the thylakoid membrane early in seedling development.C) to discover new genes that are required for correct plastoglobule function and function.