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New functions of plastoglobules in Vitamin E and K metabolism

English title New functions of plastoglobules in Vitamin E and K metabolism
Applicant Kessler Felix
Number 141229
Funding scheme Project funding (Div. I-III)
Research institution Laboratoire de Physiologie Végétale Institut de biologie Université de Neuchâtel
Institution of higher education University of Neuchatel - NE
Main discipline Cellular Biology, Cytology
Start/End 01.08.2012 - 31.07.2015
Approved amount 244'027.00
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All Disciplines (2)

Discipline
Cellular Biology, Cytology
Biochemistry

Keywords (7)

metabolism; Lipids droplets; chloroplast; vitamin E; Vitamin K; lipidomic; plastoglobules

Lay Summary (English)

Lead
Lay summary

Chloroplasts are essential organelles in plants and responsible for photosynthesis, the source of all food on our planet. Photosynthesis requires the transfer of electrons all the way from water to carbon to fix carbon dioxide and produce sugar. For this reason, the plant uses prenylquinone compounds: these include plastoquinone, phylloquinone (also known as Vitamin K) and tocopherol (also known as Vitamin E). Plastoquinone and Vitamin K are important components of electron transfer but also play essential roles as membrane antioxidants. The membrane antioxidant function is the main role of Vitamin E in plants. For the similar reasons people must take up Vitamin E and K with their food.    

In this SNF project we are trying to understand how exactly plants make and store the prenylquinone compounds. We recently discovered that a large part of the Vitamins E and K as well as plastoquinone are stored in tiny lipid droplets (called plastoglobules) inside the chloroplast. But very interestingly, the Vitamins are not only stored there but can also undergo metabolic transformation in the lipid droplets. But still there are many open questions and we would now like to understand how regulatory factors (ABC1-like kinases, NAD(P)H quinone reductase and others) regulate the production of the Vitamins in plants and how these are then transported between lipid droplets and the thylakoid membrane. We are looking forward to sharing the results of this research with you in our upcoming publications.


Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Publications

Publication
ABC1K1/PGR6 kinase: a regulatory link between photosynthetic activity and chloroplast metabolism.
Martinis Jacopo, Glauser Gaétan, Valimareanu Sergiu, Stettler Michaela, Zeeman Samuel C, Yamamoto Hiroshi, Shikanai Toshiharu, Kessler Felix (2014), ABC1K1/PGR6 kinase: a regulatory link between photosynthetic activity and chloroplast metabolism., in The Plant journal : for cell and molecular biology, 77(2), 269-83.
Border control: selectivity of chloroplast protein import and regulation at the TOC-complex
Demarsy Emilie, Lakshmanan Ashok M., Kessler Felix (2014), Border control: selectivity of chloroplast protein import and regulation at the TOC-complex, in FRONTIERS IN PLANT SCIENCE, 5, 483.
Prenylquinone profiling in whole leaves and chloroplast subfractions.
Kessler Felix, Glauser Gaetan (2014), Prenylquinone profiling in whole leaves and chloroplast subfractions., in Methods in molecular biology (Clifton, N.J.), 1153, 213-26.
Role of plastoglobules in metabolite repair in the tocopherol redox cycle
Piller Lucia Eugeni, Glauser Gaetan, Kessler Felix, Besagni Celine (2014), Role of plastoglobules in metabolite repair in the tocopherol redox cycle, in FRONTIERS IN PLANT SCIENCE, 5, 1-10.
The role of plastoglobules in thylakoid lipid remodeling during plant development
Rottet Sarah, Besagni Céline, Kessler Felix (2014), The role of plastoglobules in thylakoid lipid remodeling during plant development, in Biochimica et Biophysica Acta - Bioenergetics, 1847(9), 889-899.
A Chloroplast ABC1-like Kinase Regulates Vitamin E Metabolism in Arabidopsis
Martinis Jacopo, Glauser Gaetan, Valimareanu Sergiu, Kessler Felix (2013), A Chloroplast ABC1-like Kinase Regulates Vitamin E Metabolism in Arabidopsis, in PLANT PHYSIOLOGY, 162(2), 652-662.
A mechanism implicating plastoglobules in thylakoid disassembly during senescence and nitrogen starvation
Besagni Celine, Kessler Felix (2013), A mechanism implicating plastoglobules in thylakoid disassembly during senescence and nitrogen starvation, in PLANTA, 237(2), 463-470.
Dual targeting of a mature plastoglobulin/fibrillin fusion protein to chloroplast plastoglobules and thylakoids in transplastomic tobacco plants
Shanmugabalaji Venkatasalam, Besagni Celine, Piller Lucia Eugeni, Douet Veronique, Ruf Stephanie, Bock Ralph, Kessler Felix (2013), Dual targeting of a mature plastoglobulin/fibrillin fusion protein to chloroplast plastoglobules and thylakoids in transplastomic tobacco plants, in PLANT MOLECULAR BIOLOGY, 81(1-2), 13-25.
Chloroplast Delivery by UPS
Kessler Felix (2012), Chloroplast Delivery by UPS, in SCIENCE, 338(6107), 622-623.
Fatty Acid Phytyl Ester Synthesis in Chloroplasts of Arabidopsis
Lippold Felix, vom Dorp Katharina, Abraham Marion, Hoelzl Georg, Wewer Vera, Yilmaz Jenny Lindberg, Lager Ida, Montandon Cyrille, Besagni Celine, Kessler Felix, Stymne Sten, Doermann Peter (2012), Fatty Acid Phytyl Ester Synthesis in Chloroplasts of Arabidopsis, in PLANT CELL, 24(5), 2001-2014.
Plastid lipid droplets at the crossroads of prenylquinone metabolism
Piller Lucia Eugeni, Abraham Marion, Doermann Peter, Kessler Felix, Besagni Celine (2012), Plastid lipid droplets at the crossroads of prenylquinone metabolism, in JOURNAL OF EXPERIMENTAL BOTANY, 63(4), 1609-1618.
STAY-GREEN and Chlorophyll Catabolic Enzymes Interact at Light-Harvesting Complex II for Chlorophyll Detoxification during Leaf Senescence in Arabidopsis
Sakuraba Yasuhito, Schelbert Silvia, Park So-Yon, Han Su-Hyun, Lee Byoung-Doo, Andres Celine Besagni, Kessler Felix, Hoertensteiner Stefan, Paek Nam-Chon (2012), STAY-GREEN and Chlorophyll Catabolic Enzymes Interact at Light-Harvesting Complex II for Chlorophyll Detoxification during Leaf Senescence in Arabidopsis, in PLANT CELL, 24(2), 507-518.

Collaboration

Group / person Country
Types of collaboration
Swiss Plant Science Web Switzerland (Europe)
- Research Infrastructure
Abteilung Pflanzenbiochemie Germany (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Exchange of personnel
Prof. Michel Havaux, Centre CEA Cadarache France (Europe)
- in-depth/constructive exchanges on approaches, methods or results
Graduate School of Agriculture Japan (Asia)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Prof. S. Baginsky, Universität Halle Germany (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
Symposium of the plant science research community in Switzerland Talk given at a conference VTE5 phytol kinase is essential for resistance to combined light and temperature stress in tomato 25.01.2017 Leukerbad, Switzerland Kessler Felix;
Gordon Conference: Plant Lipids: Structure, Metabolism & Function Poster Plastoglobules: plastid lipid droplets are a key to thylakoid membrane remodeling 01.02.2015 Galveston TX, United States of America Kessler Felix;
SFB648-Seminarreihe Individual talk Plastoglobules: an unexpected site of chloroplast lipid metabolism and remodelling 15.01.2015 Halle, Germany Kessler Felix;
Plastids: differentiation and communication Talk given at a conference TOC159. A preprotein import receptor required for chloroplast biogenesis. 27.11.2014 Grenoble, France Kessler Felix;
9th Trinational Arabidopsis Meeting (TNAM) Poster Characterization of a Leucin-Rich Repeat (LRR) protein of the chloroplast envelope 24.09.2014 Heidelberg, Germany Kessler Felix;
Gordon Research Conference: Mitochondria & Chloroplasts Talk given at a conference Plastoglobule ABC1K1/PGR6 kinase: a link between photosynthetic energy production and thylakoid membrane remodelling 06.07.2014 Lucca (Barga), Italy Kessler Felix;
SWISSPLANT 2014 Symposium Individual talk ABC1K, a chloroplast kinase, couples the response of photosynthesis to highlight with metabolism 29.01.2014 Meiringen, BE, Switzerland Kessler Felix;
Seminar Seoul National University Individual talk The chloroplast life cycle: from biogenesis to senescence 10.01.2014 Seoul, Korean Republic (South Korea) Kessler Felix;


Knowledge transfer events

Active participation

Title Type of contribution Date Place Persons involved
Tech'École Performances, exhibitions (e.g. for education institutions) 01.01.2013 Neuchâtel, Switzerland Kessler Felix;


Associated projects

Number Title Start Funding scheme
127380 Discovering new regulatory mechanisms at Toc159 in the chloroplast pro-tein import machinery 01.10.2009 Project funding (Div. I-III)
156998 Exploring the chloroplast ABC1-like kinase phosphoprotein network: roles in remodeling thylakoid lipid composition, photosynthesis and beyond. 01.01.2015 Project funding (Div. I-III)
156998 Exploring the chloroplast ABC1-like kinase phosphoprotein network: roles in remodeling thylakoid lipid composition, photosynthesis and beyond. 01.01.2015 Project funding (Div. I-III)
144156 Role of phosphorylation at Toc159 in chloroplast protein import and identification of a new outer chloroplast membrane kinase 01.10.2012 Project funding (Div. I-III)
145658 Photosynthesis, Solar Energy and Sustainability 01.08.2013 Agora

Abstract

Vitamins E and K are important components of nutrition and essential for human health. Vitamin E (tocopherol group) consists of closely related lipid-soluble antioxidants. They inhibit reactive oxygen species production due to lipid peroxidation. Vitamin K (phyllo-/menaquinone group) are lipid-soluble compounds that are necessary for modification of proteins implicated in blood coagulation. Together with plastoquinone, Vitamins E and K belong to the family of prenylquinone compounds. In plants, these are synthesized mostly in the chloroplast and carry important roles as lipid antioxidants (tocopherol) and/or electron carriers in photosynthesis (phylloquinone and plastoquinone), respectively.The photosynthetic light reactions in the chloroplast take place at the thylakoid membrane and this is where the prenylquinones fulfill most of their essential roles in plants. In contrast, most of the biosynthetic reactions take place in the envelope membranes. But we recently discovered that chloroplast lipid droplets called plastglobules that tightly associate with the thylakoid membrane also play an essential role in prenylquinone metabolism. Using a proteomics approach we identified about 30 different proteins coating plastoglobules. These ranged from the structural plastoglobulins, to a number of known enzymes (including the tocopherol cyclase VTE1 required for tocopherol biosynthesis) as well as uncharacterized proteins mostly predicted to be involved in lipid metabolism. We therefore tested the hypothesis that plastoglobules are indeed implicated in lipid and more specifically in prenylquinone metabolism. Using state of the art lipidomics techniques, among others, this lead to the discovery of essential metabolic roles of plastoglobules in Vitamin E (tocopherol) and Vitamin K (phylloquinone) accumulation. More important functions certainly remain to be discovered. This project has three aims and implicates a broad range of techniques:A) to systematically unravel the function of several yet uncharacterized plastoglobule proteins. These have hypothetical functions in the regulation of prenylquinone synthesis and their trafficking between plastoglobules and the thylakoid membrane.B) to adress the hypothesis that plastoglobules are implicated in the biogenesis of the thylakoid membrane early in seedling development.C) to discover new genes that are required for correct plastoglobule function and function.
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