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Host-associated bacterial communities in Oncorhynchus mykiss: a microbiome investigation of distribution, function and coevolution

English title Host-associated bacterial communities in Oncorhynchus mykiss: a microbiome investigation of distribution, function and coevolution
Applicant Wilkins Laetitia
Number 164915
Funding scheme Early Postdoc.Mobility
Research institution
Institution of higher education Institution abroad - IACH
Main discipline Ecology
Start/End 01.09.2016 - 28.02.2018
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All Disciplines (2)

Discipline
Ecology
Zoology

Keywords (6)

Coevolution; Life-history; Microbiome; Microbiota; Oncorhynchus; Salmonid

Lay Summary (German)

Lead
1. Bilden Fische eine Lebensgemeinschaft mit den Bakterien die sie besiedeln? Alle mehrzelligen Lebewesen sind von Bakterien besiedelt, die wichtige Funktionen für ihren Träger übernehmen können. Besonders Darmbakterien spielen hier eine bedeutende Rolle. Untersuchungen solcher Bakterien führten in den letzten Jahren zu erstaunlichen Erkenntnissen. So haben verschiedene Forschungsgruppen aufgezeigt, dass diese winzig kleinen Mitbewohner nicht nur unsere Verdauung beeinflussen, sondern auch die Resistenz gegen Krankheiten und sogar den Gemütszustand. Ferner sind Bakteriengemeinschaften extrem vielfältig und unterscheiden sich vom einen Träger zum anderen stark. Folglich wissen wir wenig darüber, welche Faktoren die Vielfalt von Bakterien bestimmen und ob Bakterien an Nachkommen weitergegeben werden.
Lay summary

2. Für meine Arbeit habe ich die Regenbogenforelle in ihrem natürlichen Verbreitungsgebiet in Nordamerika ausgewählt. Denn diese Fische eignen sich besonders weil sie eine von zwei unterschiedlichen Lebensweisen auswählen: Entweder sie bleiben ihr Leben lang im Süsswasser oder sie wandern als Jungfische ins Meer, um erst für die Paarung wieder zu ihrem Ursprungsfluss zurück zu kehren. Dies führt zu physiologischen, morphologischen und verhaltenstypischen Unterschieden, welche ein spannendes System darstellen, um die Bedeutung von Bakterien für ihren Träger zu studieren. Überdies sind mit Regenbogenforellen gezielte Experimente im Labor möglich. Dort kann die Interaktion zwischen dem Träger und seinen Bakterien manipuliert werden und störende Umweltfaktoren sind ausgeschlossen. In diesem Projekt werde ich aufzeigen, wie Bakterien an Nachkommen weitergegeben werden und dabei helfen, sich an verschiedene Lebensbedingungen anzupassen. 

3. Dieses Projekt befasst sich mit Grundlagenforschung. Wir werden die Verteilung und Funktion von Bakterien in einem natürlichen Ökosystem beschreiben. Informationen aus dieser Arbeit werden es uns erlauben, die Gemeinschaft von Bakterien und Forellen besser zu verstehen und mit der Lebensgeschichte der Regenbogenforelle in Verbindung zu bringen. Erkenntnisse aus diesem Projekt können nach meiner Rückkehr in die Schweiz auch auf heimische Forellenpopulationen angewendet werden und deren Schutz und Erhaltung stärken.

Direct link to Lay Summary Last update: 22.07.2016

Responsible applicant and co-applicants

Collaboration

Group / person Country
Types of collaboration
Smithsonian Tropical Research Institute, Prof. Harilaos Lessios, Dr. Alexandra Hiller Panama (South America)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
Klamath River Project, Dr. Morgan Knechtle United States of America (North America)
- in-depth/constructive exchanges on approaches, methods or results
- Research Infrastructure
Amt für Jagd und Fischerei Graubünden AJF, Dr. Marcel Michel Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Yurok Tribe Fisheries Program, CA, Dr. Andrew Antonetti United States of America (North America)
- in-depth/constructive exchanges on approaches, methods or results
- Research Infrastructure
Carlson lab, Prof. Stephanie Carlson, UC Berkeley United States of America (North America)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Smithsonian Tropical Research Institute, Dr. Jarrod Scott, Dr. Matthieu Leray Panama (South America)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
NOAA Southwest Fisheries, UC Santa Cruz, Prof. J. Carlos Garza, Dr. Tommy Williams, Dr. Dave Rundio United States of America (North America)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
USGS CA Cooperative Fish and Wildlife Research Unit, Dr. Mark Henderson United States of America (North America)
- in-depth/constructive exchanges on approaches, methods or results
- Research Infrastructure
Marin Municipal Water District, CA, Dr. Gregory Andrews United States of America (North America)
- in-depth/constructive exchanges on approaches, methods or results
- Research Infrastructure
Eisen lab, Prof. Jonathan A. Eisen, UC Davis United States of America (North America)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
Weekly Seminar, WSL, Birmensdorf Individual talk Bacteria everywhere: on fish, inside fish, and in hot springs of Kamchatka 25.10.2017 WSL, Birmensdorf, Switzerland Wilkins Laetitia;
Invited Seminar, U.S. Fish and Wildlife Service, Red Bluff, CA Individual talk Non-genetic paternal effects on early salmonid development 05.09.2017 Red Bluff Fish and Wildlife Office, CA, USA, United States of America Wilkins Laetitia;
147th Annual Meeting of the American Fisheries Society, Tampa, Florida Talk given at a conference Non-genetic paternal effects on early salmonid development 21.08.2017 Tampa, Florida, United States of America Wilkins Laetitia;
Invited Seminar, UC Davis, Microbiology & Molecular Genetics Individual talk Diversity of bacterial symbionts on salmonid eggs - genetic and environmental effects 26.04.2017 Davis, CA, United States of America Wilkins Laetitia;
51st Annual Chapter Meeting: Navigating the Complexity of Fisheries Science, Cal-Neva, Eureka, CA Talk given at a conference Maternal allocation of carotenoids increases tolerance to bacterial infection in brown trout 05.04.2017 Eureka, Kalifornien, United States of America Wilkins Laetitia;
Invited Seminar, NOAA Southwest Fisheries, UC Santa Cruz Individual talk Salmonid embryos: a powerful system to study evolutionarily relevant questions in ecology 27.03.2017 Santa Cruz, United States of America Wilkins Laetitia;
Invited Seminar, WSL, Birmensdorf Individual talk 16 Questions in Evolutionary Biology 08.02.2017 WSL, Birmensdorf, Switzerland Wilkins Laetitia;
21st Lake Arrowhead Microbial Genomics Meeting, UCLA Poster Host-microbe evolution in rainbow trout 19.09.2016 Lake Arrowhead, UCLA, CA, United States of America Wilkins Laetitia;
Invited Seminar, Wildlife & Conservation, ESPM, UC Berkeley Individual talk 16 Questions in Evolutionary Biology 16.09.2016 Berkeley, United States of America Wilkins Laetitia;


Knowledge transfer events



Self-organised

Title Date Place
Berkeley Spouses, Partners & Parents Association Network Meetings 23.02.2017 Berkeley, United States of America

Communication with the public

Communication Title Media Place Year
Talks/events/exhibitions Evolution of aquatic animals & their associated microbes, at Ocean View Elementary School, Albany CA International 2018
Talks/events/exhibitions Freshwater Stream Outreach, Camplindo Highschool, Moraga, CA International 2018
New media (web, blogs, podcasts, news feeds etc.) The Molecular Ecologist Blog International 2017
New media (web, blogs, podcasts, news feeds etc.) My Life as A Mother Researcher Blog International 2016

Awards

Title Year
Travel Grant to attend and present at the Annual Meeting of the American National Postdoc Association (NPA) in Cleveland, 2018 2018

Abstract

Bacterial communities can play essential roles in development and function of their vertebrate hosts. This has been exemplified using model organisms under controlled laboratory conditions. However, describing the coevolution of natural hosts and their associated bacteria in the wild has turned out to be difficult due to environmental variation and stochastic effects. The Pacific coast along western North America harbors a plethora of freshwater drainages that are home to different salmonid species with particular phenotypic traits. One such trait is smoltification, the process through which fishes mature from their freshwater juvenile rearing habitats into an ocean-going phase to grow into adulthood. This transformation is the epitome of a complex phenotype, involving changes in physiological, morphological, and behavioral traits preceding downstream migration to the ocean. In the enigmatic salmonid species Oncorhynchus mykiss undergoing this transformation is optional-individuals that smolt become ocean-going “steelhead” while other individuals remain in freshwater to mature as “rainbow trout”. In this project I am taking advantage of the replication of O. mykiss populations with both phenotypes across watersheds along an environmental gradient in order to study the coevolution of host phenotype and associated gut bacteria in the wild. Host-associated bacterial communities will be characterized based on highly informative, bacteria-specific gene segments and publicly available databases about bacterial identity and function. At the conclusion of this work combined information, including different key developmental stages in the host system, will allow me to characterize variation in bacterial gut communities of O. mykiss across populations and relate this variation to migratory life-history. These results will lead to a better understanding of host - symbiont interactions in the wild and functional differences in bacterial communities between the two different host phenotypes.
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