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Evolution of moonwort ferns (Botrychium, Ophioglossaceae) on local to global scales

English title Evolution of moonwort ferns (Botrychium, Ophioglossaceae) on local to global scales
Applicant Grant Jason
Number 156456
Funding scheme Project funding (Div. I-III)
Research institution Laboratoire de Botanique Évolutive Institut de Biologie Université de Neuchâtel
Institution of higher education University of Neuchatel - NE
Main discipline Botany
Start/End 01.12.2014 - 30.11.2017
Approved amount 236'215.00
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Keywords (11)

Botrychium; phylogeography; biparental inheritance; genome scan; local adaptation; ferns; population genetics; systematics; polyploidy; landscape genetics; Ophioglossaceae

Lay Summary (French)

Lead
Le genre Botrychium appartient à la famille des fougères Ophioglossacées. Il est composé d'espèces diploïdes et polyploïdes qui vivent dans les régions montagneuses, généralement froides de l'hémisphère nord et sud. Le phénotype et la variation génétique sont importants entre espèces du même genre mais également au sein d'une même espèce. Par conséquent, il y a de gros problèmes taxonomiques de spéciation à l'échelle mondiale. Ce projet va contribuer à répondre à ces problèmes.
Lay summary

Le projet combine des approches génétiques diverses visant à une compréhension des processus de spéciation tant au niveau phylogéographique globale que de l'adaptation environnemental locale. Ce projet s'appuie sur l’accessibilité à une grande collection d’échantillons du genre Botrychium qui servira de modèle prometteur de l'évolution. En particulier, cette recherche associe des enjeux fondamentaux de relations entre taxons et des processus évolutifs clés responsables d'adaptations à des conditions environnementales particulières.

Pour la première fois, des phylogénies nucléaires et chloroplastiques regroupant une forte représentativité de chaque espèce dans le monde seront générées. Nous allons également contribuer à la caractérisation des relations entre polyploïdes et parents diploïdes. Enfin, l'utilisation d'une fougère comme modèle pour comprendre l'adaptation des populations aux composantes environnementales le long de gradients altitudinaux est une nouvelle approche. Les résultats seront d'un grand intérêt pour les biologistes de l'évolution, les systématiciens, et les biologistes de la conservation.

 

Direct link to Lay Summary Last update: 03.10.2014

Responsible applicant and co-applicants

Employees

Name Institute

Publications

Publication
Rapid allopolyploid radiation of moonwort ferns (Botrychium; Ophioglossaceae) revealed by PacBio sequencing of homologous and homeologous nuclear regions
Benjamin Dauphin Jason R. Grant Donald R. Farrar Carl J. Rothfels (2018), Rapid allopolyploid radiation of moonwort ferns (Botrychium; Ophioglossaceae) revealed by PacBio sequencing of homologous and homeologous nuclear regions, in Molecular Phylogenetics and Evolution, 120, 342-353.
A Worldwide Molecular Phylogeny Provides New Insight on Cryptic Diversity Within the Moonworts (Botrychium s. s., Ophioglossaceae)
Benjamin Dauphin Donald R. Farrar Alessio Maccagni and Jason R. Grant (2017), A Worldwide Molecular Phylogeny Provides New Insight on Cryptic Diversity Within the Moonworts (Botrychium s. s., Ophioglossaceae), in Systematic Botany, 42(4), 620-639.
Ploidy level and genome size variation in the homosporous ferns Botrychium s.l. (Ophioglossaceae)
Benjamin Dauphin Jason Grant Patrik Mraz (2016), Ploidy level and genome size variation in the homosporous ferns Botrychium s.l. (Ophioglossaceae), in Plant Systematics and Evolution, 302, 575-584.
A community-derived classification for extant lycophytes and ferns
The Pteridophyte Phylogeny Group (2016), A community-derived classification for extant lycophytes and ferns, in Journal of Systematics and Evolution, 54(6), 563-603.
Mer om pysslinglåsbräken i Sverige
Peter Ståhl Joakim Ekman Sture Westerberg Jason R. Grant & Benjamin Dauphin (2016), Mer om pysslinglåsbräken i Sverige, in Svensk Botanisk Tidskrift, 110(2), 68-74.

Collaboration

Group / person Country
Types of collaboration
Prof. Xian-Chun Zhang, Chinese Academy of Sciences, Beijing, China China (Asia)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
- Exchange of personnel
Prof. Don Farrar, Iowa State University, Ames, Iowa, USA United States of America (North America)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
Eidg. Forschungsanstalt WSL, Invited oral presentation Individual talk Evolution of the cryptic and polyploid moonwort ferns (Botrychium, Ophioglossaceae) 21.03.2018 Eidg. Forschungsanstalt WSL, Switzerland Dauphin Benjamin;
University of Zurich, Invited oral presentation in seminar series Individual talk Evolution of the cryptic and polyploid moonwort ferns (Botrychium, Ophioglossaceae) 19.03.2018 University of Zurich, Switzerland Dauphin Benjamin;
XIX International Botanical Congress (IBC 2017), Shenzhen, China Poster Reticulate evolution and parental lineage reconstruction of polyploid moonwort species (Botrychium, Ophioglossaceae) using PacBio sequencing technology 23.07.2017 Shenzhen, China Dauphin Benjamin;
XIX International Botanical Congress (IBC 2017), Shenzhen, China Talk given at a conference A worldwide molecular phylogeny of the morphologically cryptic genus Botrychium (Ophioglossaceae) 23.07.2017 Shenzhen, China Dauphin Benjamin;
University of California at Berkeley, seminar Individual talk Evolution of moonwort ferns (Botrychium, Ophioglossaceae) on local to global scales 28.03.2017 University of California at Berkeley, United States of America Dauphin Benjamin;
Conference of the American Fern Society and the Botanical Society of America. Poster Gene flow by spore dispersal vs sperm transport by subsurface water flow in outcrossing Botrychium lunaria populations in the Swiss Alps 25.07.2015 Edmonton, Canada Dauphin Benjamin;


Awards

Title Year
Outstanding Student Award, International Botanical Congress 2017, China 2017

Associated projects

Number Title Start Funding scheme
182688 Getting to the roots in the fern phylogeny - phylogenetics and biogeography of the Ophioglossaceae and the grape-ferns (Sceptridium) 01.11.2018 Project funding (Div. I-III)

Abstract

Background. The moonwort genus Botrychium of the fern family Ophioglossaceae is composed of diploid and polyploid species that occur in generally cool mountainous regions of the northern and southern hemisphere. Its species are phenotypically variable, and have slight to significant genetic variation within species, between species, or species groups. Consequently there are significant taxonomic problems on a worldwide scale. Speciation and evolutionary processes are largely unknown.General objectives. The proposed project combines phylogeographical and landscape genetic approaches to address a set of questions focusing on speciation processes on local to global scales. This research proposal builds on my knowledge and specimen accessibility in a group that will serve as a promising model of evolution. In particular, this research intends to associate one of the fundamental issues inferring relationships between taxa with key evolutionary processes responsible in adaptation to particular environmental conditions.Specific aims. Where is the center of species richness of Botrychium? Is it the same geographical area that holds the greatest genetic diversity? Have the allopolyploid taxa arisen from a single or multiple origin/event? To what extent are there maternal or paternal preferences in the formation of allopolyploids, and what is the divergence time between them? Finally, what are the spatial genetic structures, and do we have indirect evidence of adaptation to environmental components in diploid B. lunaria populations along their altitudinal ranges (from 800 to 2300 meters)?Experimental design and approaches. The first aim is to produce a new plastid phylogeny based on psbA-trnHGUG, trnLUAA-trnFGAA, and rpL16 regions. Sampling will be concentrated on previously missing species and unsampled geographical areas, especially in Asia. Five specimens of each taxon will be included in the molecular analyses to assess haplotypic diversity within taxa of the same region. The second goal is to reconstruct a nuclear phylogeny to identify parental couples responsible for the formation of the allopolyploids. Subsequently, the comparative analysis between nuclear and plastid phylogenies will allow us to determine the impact of reticulate evolution in the genus and the origin of these taxa following a single or multiple formation in the North hemisphere. The third aim is to distinguish patterns of spatial genetic structures between Swiss populations of Botrychium lunaria in order to infer indirect evidence of local adaptation. Value of the proposed project. For the first time, robust plastid and nuclear phylogenies containing all species with high worldwide representativeness will be generated. I will also attempt to characterize the relationships between polyploids and their diploid progenitors. Using a fern as a model for understanding adaptation to environmental components in populations along altitudinal ranges is a novel approach. The results will be of significant interest to evolutionary biologists, systematic biologists and conservation biologists throughout the world where the genus occurs.
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