Projekt

Zurück zur Übersicht

Tomography of microvascular structures in murine brain tumors

Titel Englisch Tomography of microvascular structures in murine brain tumors
Gesuchsteller/in Müller Bert
Nummer 144535
Förderungsinstrument Projekte
Forschungseinrichtung Zentrum für Lehre und Forschung Kantonsspital Basel Onkologie
Hochschule Universität Basel - BS
Hauptdisziplin Physik der kondensierten Materie
Beginn/Ende 01.01.2013 - 30.11.2016
Bewilligter Betrag 217'808.00
Alle Daten anzeigen

Alle Disziplinen (3)

Disziplin
Physik der kondensierten Materie
Strukturforschung
Materialwissenschaften

Keywords (6)

phase contrast tomography, vascular tree , tumour growth, segmentation, blood vessel diameter, synchrotron radiation

Lay Summary (Englisch)

Lead
The three-dimensional vascular structures down to the smallest capillaries have been of vital interest in cancer research because of the demand for alternatives to the established treatments including surgery, medication and radiation. The present research efforts range from in vivo imaging (MRI, US, and PET), via post mortem methods, including micro computed computer tomography and histology, to in silico analysis of flow behavior and vascular growth.
Lay summary

The three-dimensional vascular structures down to the smallest capillaries have been of vital interest in cancer research because  of the demand for alternatives to the established treatments including surgery, medication and radiation. The present research efforts range from in vivo imaging (MRI, US, and PET), via post mortem methods, including micro computed computer tomography and histology, to in silico analysis of flow behavior and vascular growth.

In a previous study, we showed that synchrotron radiation-based µCT (SRµCT) provides the necessary spatial resolution and contrast to capture the smallest vessels from casts. Tumors with damaged vessel walls are inappropriate for casting. Therefore, phase tomography was applied to visualize the capillaries. Grating-based tomography yields the necessary contrast but vessels with a diameter smaller than 20 µm are impossible to be segmented. In-line tomography provides the necessary spatial resolution but hardly enough contrast. Consequently, we will first improve the spatial resolution of grating-based tomography, second identify rather simple in-line tomography approaches such as the one introduced by Paganin searching for better contrast, and third combine tomograms from both approaches to gain additional information toward the smallest capillaries.

During a previous study, we developed software to convert the voxel-based tomography data to vector-based representations better suited for a detailed comparison with simulations with the aim to validate the tumor growth models. Subsequent to the data conversion, the vessel parameters of murine healthy and cancerous tissues were extracted. It has been demonstrated that the mean vessel diameter in cancerous tissue is about a factor of two smaller than for healthy tissue. We will continue and improve the vessel tree analysis while focusing on surface tortuosity, vessel density, and fractal dimension. These parameters are more important for the identification of potential cancer treatments. Therefore, we will take special attention on the capillary network of the transition zone between cancerous to healthy tissues. Further insight into the tumor formation and growth should be gained using a multimodal registration between less detailed in vivo MR and PET data and the high-resolution post mortem phase tomography.

Direktlink auf Lay Summary Letzte Aktualisierung: 21.12.2012

Verantw. Gesuchsteller/in und weitere Gesuchstellende

Mitarbeitende

Publikationen

Publikation
X-ray microscopy of soft and hard human tissues
Müller B., Schulz G., Deyhle H., Stalder A. K., Ilgenstein B., Holme M.N., Weitkamp T., Beckmann F., Hieber S.E. (2016), X-ray microscopy of soft and hard human tissues, in AIP Conference Proceedings , Melbourne, AustraliaAmerican Institute of Physics, unknown.
Grating interferometry-based phase microtomography of atherosclerotic human arteries
Buscema Marzia, Holme Margaret N., Deyhle Hans, Schulz Georg, Schmitz Ruediger, Thalmann Peter, Hieber Simone E., Chicherova Natalia, Cattin Philippe C., Beckmann Felix, Herzen Julia, Weitkamp Timm, Saxer Till, Mueller Bert (2014), Grating interferometry-based phase microtomography of atherosclerotic human arteries, in Proc. SPIE, SPIE, San Diego.
Tumors in murine brains studied by grating-based phase contrast microtomography
Schulz Georg, Dominietto Marco, Kovacs Zsofia, Schmitz Ruediger, Hieber Simone E., Thalmann Peter, Beckmann Felix, Mueller Bert (2014), Tumors in murine brains studied by grating-based phase contrast microtomography, in Proc. SPIE, SPIE, San Diego.
Three-dimensional registration of synchrotron radiation-based micro-computed tomography images with advanced laboratory micro-computed tomography data from murine kidney casts
Thalmann Peter, Hieber Simone E., Schulz Georg, Deyhle Hans, Khimchenko Anna, Kurtcuoglu Vartan, Olgac Ufuk, Marmaras Anastasios, Kuo Willy, Meyer Eric P., Beckmann Felix, Herzen Julia, Ehrbar Stefanie, Mueller Bert (2014), Three-dimensional registration of synchrotron radiation-based micro-computed tomography images with advanced laboratory micro-computed tomography data from murine kidney casts, in Proc. SPIE, SPIE, San Diego.
Three-dimensional imaging of human hippocampal tissue using synchrotron radiation- and grating-based micro computed tomography
Hieber Simone E., Khimchenko Anna, Kelly Christopher, Mariani Luigi, Thalmann Peter, Schulz Georg, Schmitz Ruediger, Greving Imke, Dominietto Marco, Mueller Bert (2014), Three-dimensional imaging of human hippocampal tissue using synchrotron radiation- and grating-based micro computed tomography, in Proc. SPIE, SPIE, San Diego.
Augmented reality assisted brain tumor extraction in mice
Schneider Adrian, Thalmann Peter, Pezold Simon, Hieber Simone E., Cattin Philippe C. (2014), Augmented reality assisted brain tumor extraction in mice, in Lecture Notes in Computer Science , 9254, 255-264.
Combined use of micro computed tomography and histology to evaluate the regenerative capacity of bone grafting materials
Stalder Anja K., Ilgenstein Bernd, Chicherova Natalia, Deyhle Hans, Beckmann Felix, Mueller Bert, Hieber Simone E. (2014), Combined use of micro computed tomography and histology to evaluate the regenerative capacity of bone grafting materials, in INTERNATIONAL JOURNAL OF MATERIALS RESEARCH, 105(7), 679-691.
Grating interferometry-based phase microtomography of atherosclerotic human arteries
Buscema Marzia, Holme Margaret N., Deyhle Hans, Schulz Georg, Schmitz Ruediger, Thalmann Peter, Hieber Simone E., Chicherova Natalia, Cattin Philippe C., Beckmann Felix, Herzen Julia, Weitkamp Timm, Saxer Till, Mueller Bert (2014), Grating interferometry-based phase microtomography of atherosclerotic human arteries, in DEVELOPMENTS IN X-RAY TOMOGRAPHY IX, 9212, 921203.
Tumors in murine brains studied by grating-based phase contrast microtomography
Schulz Georg, Dominietto Marco, Kovacs Zsofia, Schmitz Ruediger, Hieber Simone E., Thalmann Peter, Beckmann Felix, Mueller Bert (2014), Tumors in murine brains studied by grating-based phase contrast microtomography, in DEVELOPMENTS IN X-RAY TOMOGRAPHY IX, 9212, 92120Q.
Three-dimensional registration of synchrotron radiation-based micro-computed tomography images with advanced laboratory micro-computed tomography data from murine kidney casts
Thalmann Peter, Hieber Simone E., Schulz Georg, Deyhle Hans, Khimchenko Anna, Kurtcuoglu Vartan, Olgac Ufuk, Marmaras Anastasios, Kuo Willy, Meyer Eric P., Beckmann Felix, Herzen Julia, Ehrbar Stefanie, Mueller Bert (2014), Three-dimensional registration of synchrotron radiation-based micro-computed tomography images with advanced laboratory micro-computed tomography data from murine kidney casts, in DEVELOPMENTS IN X-RAY TOMOGRAPHY IX, 9212, 92120Y.
Three-dimensional imaging of human hippocampal tissue using synchrotron radiation- and grating-based micro computed tomography
Hieber Simone E., Khimchenko Anna, Kelly Christopher, Mariani Luigi, Thalmann Peter, Schulz Georg, Schmitz Ruediger, Greving Imke, Dominietto Marco, Mueller Bert (2014), Three-dimensional imaging of human hippocampal tissue using synchrotron radiation- and grating-based micro computed tomography, in DEVELOPMENTS IN X-RAY TOMOGRAPHY IX, 9212, 92120S.
High-resolution synchrotron radiation-based phase tomography of the healthy and epileptic brain
Bikis Christos, Janz Philipp, Schulz Georg, Schweighauser Gabriel, Hench Juergen, Thalmann Peter, Chicherova Natalia, Rack Alexander, Khimchenko Anna, Hieber Simone E, Mariani Luigi, Haas Carola A., Müller Bert (2016), High-resolution synchrotron radiation-based phase tomography of the healthy and epileptic brain, in DEVELOPMENTS IN X-RAY TOMOGRAPHY X , 996706-11.
Comparing natural and artificial carious lesions in human crowns by means of conventional hard x-ray micro-tomography and two-dimensional x-ray scattering with synchrotron radiation
Botta Lea Maria, White Shane N., Deyhle Hans, Dziadowiec Iwona, Schulz Georg, Thalmann Peter, Müller Bert (2016), Comparing natural and artificial carious lesions in human crowns by means of conventional hard x-ray micro-tomography and two-dimensional x-ray scattering with synchrotron radiation, in Developments in X-Ray Tomography X, 99670S-11.
Imaging tissues for biomedical research using the high-resolution micro-tomography system nanotom® m
Deyhle Hans, Schulz Georg, Khimchenko Anna, Bikis Christos, Hieber Simone E., Jaquiery Claude, Kunz Christoph, Müller-Gerbl Magdalena, Höchel Sebastian, Saxer Till, Stalder Anja K., Ilgenstein Bernd, Beckmann Felix, Thalmann Peter, Buscema Marzia, Rohr Nadja, Holme Margaret N., Müller Bert (2016), Imaging tissues for biomedical research using the high-resolution micro-tomography system nanotom® m, in Developments in X-Ray Tomography X, 99670Q-12.
Computational cell quantification in the human brain tissues based on hard x-ray phase-contrast tomograms
Hieber Simone E., Bikis Christos, Khimchenko Anna, Schulz Georg, Deyhle Hans, Thalmann Peter, Chicherova Natalia, Rack Alexander, Zdora Marie-Christine, Zanette Irene, Schweighauser Gabriel, Hench Jürgen, Müller Bert (2016), Computational cell quantification in the human brain tissues based on hard x-ray phase-contrast tomograms, in Developments in X-Ray Tomography X, 99670K-12.
X-ray micro-tomography for investigations of brain tissues on cellular level
Khimchenko Anna, Schulz Georg, Deyhle Hans, Thalmann Peter, Zanette Irene, Zdora Marie-Christine, Bikis Christos, Hipp Alexander, Hieber Simone E., Schweighauser Gabriel, Hench Jürgen, Müller Bert (2016), X-ray micro-tomography for investigations of brain tissues on cellular level, in Developments in X-Ray Tomography X, 996703-10.
Hierarchical imaging of the human knee
Schulz Georg, Götz Christian, Deyhle Hans, Müller-Gerbl Magdalena, Zanette Irene, Zdora Marie-Christine, Khimchenko Anna, Thalmann Peter, Rack Alexander, Müller Bert (2016), Hierarchical imaging of the human knee, in Developments in X-Ray Tomography X,, 99670R-12.
Single and double grating-based X-ray microtomography using synchrotron radiation
Thalmann P., Bikis C., Hipp A., Müller B., Hieber S. E., Schulz G. (2017), Single and double grating-based X-ray microtomography using synchrotron radiation, in Applied Physics Letters, 110(6), 061103-061103.
Hard X-ray submicrometer tomography of human brain tissue at Diamond Light Source
Khimchenko A., Bikis C., Schulz G., Zdora M.C., Zanette I., Vila-Comamala J., Schweighauser G., Hench J., Hieber S.E., Deyhle H., Thalmann P., Müller B. (accepted), Hard X-ray submicrometer tomography of human brain tissue at Diamond Light Source, in Journal of Physics: Conference Series, IOP Publishing, UK.
Multimodal imaging of the human knee down to the cellular level
M-C. Zdora A. Khimchenko H. Deyhle P. Thalmann, Götz Christian, Müller-Gerbl Magdalena, Zanette Irene, Zdora Marie-Christine, Khimchenko Anna, Deyhle Hans, Thalmann Peter, Müller Bert (accepted), Multimodal imaging of the human knee down to the cellular level, in IOP Publishing, Journal of Physics: Conference Series , UK.

Zusammenarbeit

Gruppe / Person Land
Felder der Zusammenarbeit
ITIS (Dr. Esra Neufeld) Schweiz (Europa)
- vertiefter/weiterführender Austausch von Ansätzen, Methoden oder Resultaten
Synchr. Soleil (Dr. Timm Weitkamp) Frankreich (Europa)
- vertiefter/weiterführender Austausch von Ansätzen, Methoden oder Resultaten
- Publikation
- Forschungsinfrastrukturen
Oncology/Children Hospital ZH (Dr. Michele Bernasconi) Schweiz (Europa)
- vertiefter/weiterführender Austausch von Ansätzen, Methoden oder Resultaten
IBT/ETHZ (Prof. Dr. Markus Rudin) Schweiz (Europa)
- Publikation
- Forschungsinfrastrukturen
- Austausch von Mitarbeitern

Wissenschaftliche Veranstaltungen

Aktiver Beitrag

Titel Art des Beitrags Titel des Artikels oder Beitrages Datum Ort Beteiligte Personen
XNPIG 2015 Vortrag im Rahmen einer Tagung Multi-modal hard X-ray phase tomography of human brain tissues 08.09.2015 NIH, Bethesda, Maryland, USA, Vereinigte Staaten von Amerika Müller Bert; Hieber Simone; Thalmann Peter
CLINAM Vortrag im Rahmen einer Tagung Ex vivo imaging of brain tissue in health and disease (invited talk) 29.06.2015 Basel, Schweiz Hieber Simone; Thalmann Peter; Müller Bert
Swiss Society of Biomaterials and Regenerative Medicine Annual Meeting Einzelvortrag Texture and shape quantification to characterize angiogenesis in tumour tissue 09.06.2015 Lausanne, Schweiz Thalmann Peter; Müller Bert; Hieber Simone
Invited talk at Diamond Light Source Einzelvortrag Hard X-ray imaging of biomaterials and human tissues 10.04.2015 Didcot, Grossbritannien und Nordirland Hieber Simone; Müller Bert
International Symposium on BioMedical Applications of X-Ray Phase Contrast Imaging (IMXP) Poster Quantification of vessel morphologies in healthy and cancerous tissues using phase-contrast, hard X-ray microtomography 29.01.2015 Garmisch, Deutschland Müller Bert; Thalmann Peter; Hieber Simone
DESY Photon Science Users' Meeting Poster Quantification of vessel morphologies in healthy and cancerous tissues using phase-contrast, hard X-ray microtomography 29.01.2015 Hamburg, Deutschland Thalmann Peter; Hieber Simone; Müller Bert
International Symposium on BioMedical Applications of X-Ray Phase Contrast Imaging (IMXP) Poster Three-dimensional imaging of hippocampal tissue using synchrotron radiation grating-based micro computed tomography 29.01.2015 Garmisch, Deutschland Thalmann Peter; Hieber Simone; Müller Bert
International Symposium on BioMedical Applications of X-Ray Phase Contrast Imaging (IMXP) Poster Complementary X-ray tomography techniques for histology-validated 3D imaging of soft and hard tissues using plaque-containing blood vessels as example 29.01.2015 Garmisch, Deutschland Hieber Simone; Müller Bert; Thalmann Peter
SPIE Optics and Photonics Vortrag im Rahmen einer Tagung Three-dimensional registration of synchrotron radiation-based micro-computed tomography images with advanced laboratory micro-computed tomography data from murine kidney casts 18.08.2014 Diego, CA, Vereinigte Staaten von Amerika Hieber Simone; Thalmann Peter; Müller Bert
SPIE Optics and Photonics Vortrag im Rahmen einer Tagung Three-dimensional imaging of human hippocampal tissue using synchrotron radiation- and grating-based micro computed tomography 18.08.2014 San Diego, CA, Vereinigte Staaten von Amerika Hieber Simone; Thalmann Peter; Müller Bert


Verbundene Projekte

Nummer Titel Start Förderungsinstrument
133802 Micro- and Nanotomography 01.03.2011 R'EQUIP
147172 Micro- and nanoanatomy of human brain tissues 01.09.2013 Interdisziplinäre Projekte
153523 HR-Kidney - High Resolution 3D Functional Anatomy Database of the Kidney 01.06.2014 Projekte
150164 Multi-modal matching of two-dimensional images with three-dimensional data in the field of biomedical engineering 01.11.2013 Projekte
125406 High-resolution 3D imaging of the human brain post mortem 01.04.2009 Interdisziplinäre Projekte
127297 High-resolution phase contrast micro computed tomography of soft tissues 01.10.2009 Projektförderung (Abt. I-III)

Abstract

The three-dimensional vascular structures down to the smallest capillaries have been of vital interest in cancer research because of the demand for alternatives to the established treatments (surgery, medication and radiation). The present research efforts range from in vivo imaging (MRI, US, and PET), via post mortem methods, including micro computed computer tomography (µCT) and histology, to in silico analysis of flow behavior and vascular growth. In the previous study, we showed that synchrotron radiation-based µCT (SRµCT) provides the necessary spatial resolution and contrast to capture the smallest vessels from casts. Tumors with damaged vessel walls are inappropriate for casting. Therefore, phase tomography was applied to visualize the capillaries. Grating-based tomography yields the necessary contrast but vessels with a diameter smaller than 20 µm are impossible to be segmented. Inline tomography provides the necessary spatial resolution but hardly enough contrast. Consequently, we propose first to improve the spatial resolution of grating-based tomography, second to identify rather simple inline tomography approaches such as the one introduced by Paganin searching for better contrast, and third to combine tomograms from both approaches to gain additional information toward the smallest capillaries. During the previous study, we developed software to convert the voxel-based tomography data to vector-based representations better suited for a detailed comparison with simulations with the aim to validate the tumor growth models. Subsequent to the data conversion, the vessel parameters of murine healthy and cancerous tissues were extracted. It was demonstrated that the mean vessel diameter in cancerous tissue is about 50% smaller than for healthy tissue. We propose to continue and improve the vessel tree analysis while focusing on surface tortuosity, vessel density, and fractal dimension. These parameters are more important for the identification of potential cancer treatments. Therefore, we will take special attention on the capillary network of the transition zone between cancerous to healthy tissues. Further insight into the tumor formation and growth should be gained using a multimodal registration between less detailed in vivo MR and PET data and the high-resolution post mortem SRµCT. In order to confine the research activities, we will only study brain tumors in a mouse model established at the ETH/University of Zürich. First, we will study the entire mouse brain including tumor. Once the individual steps of imaging and analysis are arranged, we will study the impact of treatments to discover together with our partners strategies against cancer.