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Ozga Aleksandra J, Moalli Federica, Abe Jun, Swoger Jim, Sharpe James, Zehn Dietmar, Kreutzfeldt Mario, Merkler Doron, Ripoll Jorge, Stein Jens V (2016), pMHC affinity controls duration of CD8+ T cell-DC interactions and imprints timing of effector differentiation versus expansion., in The Journal of experimental medicine
Ultramicroscopy (also known as selective plane illumination microscopy, or SPIM) is a novel technique that allows optical sectioning and 3D reconstruction of large, centimeter sized, fluorescently labeled biological specimen with (sub)micrometer resolution. It is based on light sheet illumination and perpendicular fluorescence detection in biological specimen that are made transparent by a clearing procedure. This technique is very versatile and can be applied to any biological specimen. We propose to set up the first commercially available Ultramicroscope in Switzerland and to adapt it to biological questions in immunology and neurobiology. The Ultramicroscope is uniquely suited for the investigation of rare immune cells during adaptive immune responses, and the anatomy of neuronal networks in whole brains. The Ultramicroscopy setup will form part of the “Microscopy Imaging Center” (MIC) platform already established at the University of Bern, thus being available to interested researchers on a local, national and international level.