cell migration; immune cell trafficking; inflammation; tumour metastasis; tumour invasion; inflammation; live cell imaging
Priebe Magdalena, Widmer Jérôme, Suhartha Löwa Nina, Abram Sarah-Luise, Mottas Inès, Woischnig Anne-Kathrin, Brunetto Priscilla S, Khanna Nina, Bourquin Carole, Fromm Katharina M (2016), Antimicrobial silver-filled silica nanorattles with low immunotoxicity in dendritic cells., in Nanomedicine : nanotechnology, biology, and medicine
, 13(1), 11-22.
Cecchinato Valentina, D'Agostino Gianluca, Raeli Lorenzo, Uguccioni Mariagrazia (2016), Chemokine interaction with synergy-inducing molecules: fine tuning modulation of cell trafficking, in JOURNAL OF LEUKOCYTE BIOLOGY
, 99(6), 851-855.
Abe Jun, Ozga Aleksandra J, Swoger Jim, Sharpe James, Ripoll Jorge, Stein Jens V (2016), Light sheet fluorescence microscopy for in situ cell interaction analysis in mouse lymph nodes., in Journal of immunological methods
, 431, 1-10.
Lazarevic Ivana, Engelhardt Britta (2016), Modeling immune functions of the mouse blood-cerebrospinal fluid barrier in vitro: primary rather than immortalized mouse choroid plexus epithelial cells are suited to study immune cell migration across this brain barrier., in Fluids and barriers of the CNS
, 13, 2-2.
Ozga Aleksandra J, Moalli Federica, Abe Jun, Swoger Jim, Sharpe James, Zehn Dietmar, Kreutzfeldt Mario, Merkler Doron, Ripoll Jorge, Stein Jens V (2016), pMHC affinity controls duration of CD8+ T cell-DC interactions and imprints timing of effector differentiation versus expansion., in The Journal of experimental medicine
, 213(12), 2811-2829.
Rudolph Henriette, Klopstein Armelle, Gruber Isabelle, Blatti Claudia, Lyck Ruth, Engelhardt Britta (2016), Postarrest stalling rather than crawling favors CD8(+) over CD4(+) T-cell migration across the blood-brain barrier under flow in vitro., in European journal of immunology
, 46(9), 2187-203.
Tietz Silvia M, Zwahlen Marcel, Haghayegh Jahromi Neda, Baden Pascale, Lazarevic Ivana, Enzmann Gaby, Engelhardt Britta (2016), Refined clinical scoring in comparative EAE studies does not enhance the chance to observe statistically significant differences., in European journal of immunology
, 46(10), 2481-2483.
Köchl Robert, Thelen Flavian, Vanes Lesley, Brazão Tiago F, Fountain Kathryn, Xie Jian, Huang Chou-Long, Lyck Ruth, Stein Jens V, Tybulewicz Victor L J (2016), WNK1 kinase balances T cell adhesion versus migration in vivo., in Nature immunology
, 17(9), 1075-83.
Ackerknecht Markus, Hauser Mark A., Legler Daniel F., Stein Jens V. (2015), In vivo TCR signaling in CD4+
T cells imprints a cell-intrinsic, transient low-motility pattern independent of chemokine receptor expression levels, or microtubular network, integrin, and protein kinase C activity, in Frontiers in Immunology
, 6(JUN), 297.
Tang Fengyuan, Gill Jason, Ficht Xenia, Barthlott Thomas, Cornils Hauke, Schmitz-Rohmer Debora, Hynx Debby, Zhou Dawang, Zhang Lei, Xue Gongda, Grzmil Michal, Yang Zhongzhou, Hergovich Alexander, Hollaender Georg A, Stein Jens V, Hemmings Brian A, Matthias Patrick (2015), The kinases NDR1/2 act downstream of the Hippo homolog MST1 to mediate both egress of thymocytes from the thymus and lymphocyte motility., in Science signaling
, 8(397), 100-100.
Hotz Christian, Roetzer Laurin C., Huber Thomas, Sailer Andreas, Oberson Anne, Treinies Marina, Heidegger Simon, Herbst Tina, Endres Stefan, Bourquin Carole (2015), TLR and RLR Signaling Are Reprogrammed in Opposite Directions after Detection of Viral Infection, in JOURNAL OF IMMUNOLOGY
, 195(9), 4387-4395.
Abadier Michael, Abadier Michael, Haghayegh Jahromi Neda, Haghayegh Jahromi Neda, Cardoso Alves Ludmila, Boscacci Rémy, Vestweber Dietmar, Barnum Scott, Deutsch Urban, Engelhardt Britta, Lyck Ruth (2014), Cell surface levels of endothelial ICAM-1 influence the transcellular or paracellular T-cell diapedesis across the blood-brain barrier, in European Journal of Immunology
, 45(4), 1043-1058.
Sathiyanadan Karthik, Coisne Caroline, Enzmann Gaby, Deutsch Urban, Engelhardt Britta (2014), PSGL-1 and E/P-selectins are essential for T-cell rolling in inflamed CNS microvessels but dispensable for initiation of EAE., in European journal of immunology
, 44(8), 2287-94.
Moretti Federico A, Moser Markus, Lyck Ruth, Abadier Michael, Ruppert Raphael, Engelhardt Britta, Fässler Reinhard (2013), Kindlin-3 regulates integrin activation and adhesion reinforcement of effector T cells., in Proceedings of the National Academy of Sciences of the United States of America
, 110(42), 17005-10.
Coelho Fernanda M, Natale Daniela, Soriano Silvia F, Hons Miroslav, Swoger Jim, Mayer Jürgen, Danuser Renzo, Scandella Elke, Pieczyk Markus, Zerwes Hans-Günter, Junt Tobias, Sailer Andreas W, Ludewig Burkhard, Sharpe James, Figge Marc Thilo, Stein Jens V (2013), Naive B-cell trafficking is shaped by local chemokine availability and LFA-1-independent stromal interactions., in Blood
, 121(20), 4101-9.
Yan Yiqing, Jiang Wei, Spinetti Thibaud, Tardivel Aubry, Castillo Rosa, Bourquin Carole, Guarda Greta, Tian Zhigang, Tschopp Jurg, Zhou Rongbin (2013), Omega-3 fatty acids prevent inflammation and metabolic disorder through inhibition of NLRP3 inflammasome activation., in Immunity
, 38(6), 1154-63.
Marczynska Joanna, Ozga Aleksandra, Wlodarczyk Agnieszka, Majchrzak-Gorecka Monika, Kulig Paulina, Banas Magdalena, Michalczyk-Wetula Dominika, Majewski Pawel, Hutloff Andreas, Hutloff Andreas, Schwarz Jeanette, Chalaris Athena, Scheller Jürgen, Scheller Jürgen, Rose-John Stefan, Cichy Joanna (2013), The role of metalloproteinase ADAM17 in regulating ICOS ligand-mediated humoral immune responses, in Journal of Immunology
, 193(6), 2753-2763.
Nitschké Maximilian, Aebischer David, Abadier Michael, Haener Simone, Lucic Matije, Vigl Benjamin, Luche Hervé, Fehling Hans Jörg, Biehlmaier Oliver, Lyck Ruth, Halin Cornelia (2012), Differential requirement for ROCK in dendritic cell migration within lymphatic capillaries in steady-state and inflammation., in Blood
, 120(11), 2249-58.
Harada Y, Tanaka Y, Terasawa M, Pieczyk M, Habiro K, Katakai T, Hanawa-Suetsugu K, Kukimoto-Niino M, Nishizaki T, Shirouzu M, Duan XF, Uruno T, Nishikimi A, Sanematsu F, Yokoyama S, Stein JV, Kinashi T, Fukui Y (2012), DOCK8 is a Cdc42 activator critical for interstitial dendritic cell migration during immune responses, in BLOOD
, 119(19), 4451-4461.
Mayer Jürgen, Swoger Jim, Ozga Aleksandra J, Stein Jens V, Sharpe James (2012), Quantitative measurements in 3-dimensional datasets of mouse lymph nodes resolve organ-wide functional dependencies., in Computational and mathematical methods in medicine
, 2012, 128431-128431.
Sallusto F, Impellizzieri D, Basso C, Laroni A, Uccelli A, Lanzavecchia A, Engelhardt B (2012), T-cell trafficking in the central nervous system, in IMMUNOLOGICAL REVIEWS
, 248, 216-227.
Lyck R., Abadier M., Wyss C.B., Sallusto F., Engelhardt B., ALCAM (CD166) is involved in extravasation of monocytes rather than T cells across the blood–brain barrier, in J Cereb Blood Flow Metab
Cecchinato V, Uguccioni M, D'Agostino G, Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization., in The Journal of Immunology
The ProDoc Cell Migration is an initiative of the Theodor Kocher Institute (TKI) at the University of Bern (UNIBE), the Institute for Research in Biomedicine (IRB) in Bellinzona and the Chairs of Pathology and Pharmacology of the Faculty of Science at the University of Fribourg (UNIFR). Combining the research focus of the TKI on studying immune cell migration during immunosurveillance and inflammation by means of live cell imaging with the strong background on mouse and human immunology of the IRB, successful research collaborations between the laboratories of Britta Engelhardt and Jens V. Stein (TKI) and Federica Sallusto (IRB) have already been pursued. The recent recruitment of Curzio Rüegg and Carole Bourquin to UNIFR, together with the expertise of Mariagrazia Uguccioni (IRB) on expression of chemokines in tumor microenvironments regulating infiltration of immune cells and positioning of malignant cells, and of Urban Deutsch on the role of the Angiopoietin/Tie-2 in vascular biology and cancer, broadens our expertise to tumor cell biology and thus provides a profound basis for proposing the comprehensive ProDoc Cell Migration. Our complementary scientific expertises, the combination of in vivo mouse models and in vitro human models and the variety of technical platforms provide a unique framework for establishing an internationally visible training program for highly qualified PhD and MD/PhD students in the field of cell migration, the excellence of which could not be achieved at this level in the individual laboratories as such. Although the official language of the ProDoc will be English, the location of the participating institutions promotes the exposure of ProDoc students to the rich cultural diversity within Switzerland and will favor the acquisition of new languages (German, Italian and French) and thus complement the education of the students in view of future postdoctoral training and also support mobility. Within the ProDoc Cell Migration students will be grouped in two Research Modules, namely Research Module I: Immune cell migration during immunosurveillance and inflammation and Research Module II: Cell migration in tumorigenesis and metastasis, respectively. They will perform collaborative high quality research projects covering highly relevant aspects of cell migration. The six students, whose funding is requested in this application will perform specific parts of their PhD thesis within the different participating laboratories and will therefore provide the continuous and direct link within the ProDoc. Conceptually, the ProDoc Cell Migration is designed to convey to participating students in depth knowledge of the molecular events that control cell migration in both, mouse and human systems, and to confront them with a large armamentarium of state-of-the art technologies to study the questions at hand. The accompanying Training Module will be organized by Marlene Wolf, the coordinator of the interfaculty Graduate School for Cellular and Biological Sciences (GCB, www.gcb.unibe.ch) at UNIBE with 250 students enrolled currently. This module composed of lectures, practical classes, journal clubs, webportal-based training modules and meetings on the specific topics, is designed to guarantee the continuous interaction of the ProDoc students and to challenge them to become critical young researchers with professional communication skills and a strong desire for excellence as their future basis to promote their own ideas in research. In addition to the five students applied for here, eight students funded by other sources, including two national and international collaborative projects, will join the training module and benefit from all training activities and from the mobility and interactions offered within the ProDoc Cell Migration. Coordination of all research and training activities within the proposed ProDoc Cell Migration will be the obligation of the ProDoc steering committee B. Engelhardt (TKI), F. Sallusto (IRB), C. Rüegg (UNIFR) and M. Wolf (TKI), representing the institutions involved. Administration of the ProDoc Cell Migration will rely on the well established GCB at UNIBE, which will formally evaluate and approve applicants selected by the ProDoc steering committee. ProDoc students from TKI and IRB will be formally enrolled in this program, further strengthening the already established collaboration in postgraduate education between the two institutions. A strong teaching collaboration between UNIBE and UNIFR (BEFRI) is already in place in the context of the Master in Biomedical Sciences (BMS), a Graduate Program in Developmental Neurobiology and the recently planned Master Program in Bioinformatics, where the curriculum is shared between the universities. The establishment of the ProDoc Cell Migration would allow an extension of the BEFRI collaboration at the graduate level in the life sciences and should be instrumental in initiating a Graduate School in life sciences at UNIFR and enforcing the coordination of graduate studies between these universities.