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Relations between ideal and physical knots

Applicant Stasiak Andrzej
Number 68151
Funding scheme Project funding (Div. I-III)
Research institution Laboratoire d'Analyse Ultrastructurale Centre de Microscopie Electronique Université de Lausanne
Institution of higher education University of Lausanne - LA
Main discipline Biophysics
Start/End 01.01.2003 - 30.09.2006
Approved amount 223'300.00
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All Disciplines (2)

Discipline
Biophysics
Mathematics

Keywords (6)

REPLICATION; REPAIR; RECA; RECOMBINATION; ESCHERICHIA COLI; DNA

Lay Summary (English)

Lead
Lay summary
DNA molecules are long polymers and as such as prone to get knotted, however knots interfere with normal functions of DNA. The natural level of DNA knotting is orders of magnitude lower than this at thermodynamic equilibrium and it is presently unknown how DNA topoisomerases perform intersegmental DNA-DNA passages in such a way that unknotting is strongly favored over knotting. Understanding the relation between the topology (type of DNA knot) and geometry (shape) of knotted DNA molecules should help to understand how DNA topoisomerases effectively remove knots from DNA. In addition, understanding how the type of DNA knot affects electrophoretic migration of knotted DNA molecules should help to characterize naturally formed knots using standard gel electrophoretic methods without resorting to complex electron microscopy approach.
Direct link to Lay Summary Last update: 21.02.2013

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Associated projects

Number Title Start Funding scheme
61636 Relations between ideal and physical knots 01.10.2000 Project funding (Div. I-III)

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