Back to overview

From Cells to Tissues: A lattice light sheet microscope to improve spatiotemporal resolution

English title From Cells to Tissues: A lattice light sheet microscope to improve spatiotemporal resolution
Applicant Vogel Viola
Number 183525
Funding scheme R'EQUIP
Research institution Angewandte Mechanobiologie Gesundheitswissenschaften und Technologie ETH Zürich
Institution of higher education ETH Zurich - ETHZ
Main discipline Cellular Biology, Cytology
Start/End 01.10.2019 - 30.09.2020
Approved amount 470'000.00
Show all

All Disciplines (2)

Cellular Biology, Cytology

Keywords (4)

Lattice Light Sheet Microscopy; Single Molecule Microscopy; Life-Cell Microscopy; Super-Resolution Imaging

Lay Summary (German)

Ein Gitterlichtblattmikroskop - Lattice Light Sheet Microscope für eine verbesserte räumliche und zeitliche Auflösung wird als erstes Mikroskop seiner Art an der ETH Zürich installiert, um neuartige Einblicke in das Nano-Leben von Zellen und Gewebe zu gewinnen.
Lay summary

Die Funktionsweise der supra-molekularen Mechanismen, die das innere Leben von Zellen antreiben und regulieren lässt sich meist nicht allein anhand von Standbildern rekonstruieren. Deshalb revolutioniert die Bildgebung mit einer Submikrometerauflösung in lebenden Zellen bis hin zum Gewebe, wie sie bisher nur durch Gitterlichtblattmikroskopie -Lattice Light Sheet Microscope (LLSM) machbar ist, die Zellbiologie und die Bio-Nanotechnologie. Um die räumlich-zeitliche Auflösung der optischen Mikroskopie zu erhöhen, wird am ScopeM der ETH Zürich ein solches Mikroskop installiert. Dieses in der Schweiz einmalige Instrument wird es ETH Wissenschaftlern, aber auch externen Nutzern erlauben, neuartige Einblicke und Erkenntnisse zu gewinnen.  Es wurden Projekte vorgeschlagen, von der Zellbiologie und Immunologie bis hin zur Neurobiologie, aber auch im Bereich des Tissue Engineerings.

Das LLSM hat ein hohes Potential bald ebenso unverzichtbar in der Biomedizinischen Bildgebung zu werden, wie die bewährte konfokale Mikroskopie, allerdings mit viel höherer Auflösung. Dies wird durch die Krönung der Technik zur Methode des Jahres 2014 durch Nature Methods einer führenden internationalen Zeitschrift belegt. 

Direct link to Lay Summary Last update: 07.12.2018

Responsible applicant and co-applicants

Project partner

Natural persons

Name Institute

Associated projects

Number Title Start Funding scheme
169207 Interaction of graphene related materials and abraded graphene related materials reinforced nanocomposites with 3D lung cell models 01.03.2017 Project funding (Div. I-III)
175839 Mechanobiology of Extracellular Matrix 01.10.2017 Project funding (Div. I-III)
179275 Structure and Function of the Nuclear Pore Complex 01.04.2018 Project funding (Div. I-III)
169925 Control of chromosome condensation during mitosis and its role in chromatin resetting 01.01.2017 Project funding (Div. I-III)
165670 Advanced microscopy for direct observation of tendon cell and tissue mechanotransduction 01.04.2016 Project funding (Div. I-III)


Deciphering how the supramolecular machineries of cells work cannot be reconstructed from still images alone: life-cell imaging at submicron resolution, as enabled so far only by lattice light sheet microscopy, is thus prone to revolutionize cell biology and bio-nanotechnology. To increase the spatio-temporal resolution of optical microscopy, we here request funds to purchase a com-mercially available Lattice Light Sheet Microscope (LLSM) system to be operated by the well man-aged user facility ScopeM, the Scientific Center for Optical and Electron Microscopy, which is the central scientific technology platform and core facility of ETH Zurich for microscopy. This new instrument will provide the scientific research community and ScopeM users with access to a state of the art LLSM with high-speed three-dimensional superresolution imaging capabilities and low phototoxicity due to its ultra-fast acquisition speed. LLSM is currently revolutionizing biological imaging research and is quickly becoming as indis-pensable as the confocal microscope, evidenced by light-sheet imaging being named Method of the Year for 2014 by the leading journal Nature Methods. It also allows for high-speed 3D imag-ing of tissue sections. A LLSM for live-cell microscopy with minimal phototoxicity, high spatio-temporal resolution and large field of views will be implemented [1]. Moreover a structured illumi-nation (SIM) mode leads to a two-fold resolution increase in two dimensions. Good commercial products are entering the market including the improved Version 2.0 of 3I (3i-Intelligent Imaging Innovations) which we here selected as it is superior to meeting the requirements of our users. The final choice will be made on the base of a WTO tender, but - at this point in time - the Ver-sion 2.0 of 3I is the preferred/targeted instrument. Remarkably, even though several light sheet microscopes (LSM) exist in Switzerland, not a single Lattice LSM exists so far in Switzerland, to the best of our knowledge. Our goal is thus to set-up the first LLSM in Switzerland and to make it available to a broad range of users. Although ScopeM currently offers users access to a vast set of imaging techniques, spanning from confocal microscopy, structured illumination (SIM), spinning disc microscopy, stimulated emis-sion depletion (STED) and further super resolution techniques such as Stochastic Optical Re-construction Microscopy (STORM), no available system can provide the high speed 3D imaging modalities with sub-cellular resolution of the proposed LLSM. Indeed presently researchers inter-ested in using such technique/instrument must seek access abroad! This is certainly an unbear-able constrain/limitation to all of us wanting to push the frontiers in science.