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Installing a Hyperion CyTOF mass cytometry platform for high-dimensional single cell analysis at the University of Bern

English title Installing a Hyperion CyTOF mass cytometry platform for high-dimensional single cell analysis at the University of Bern
Applicant Keogh-Stroka Deborah
Number 183501
Funding scheme R'EQUIP
Research institution Department for BioMedical Research Universität Bern
Institution of higher education University of Berne - BE
Main discipline Immunology, Immunopathology
Start/End 01.10.2019 - 30.09.2020
Approved amount 759'800.00
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All Disciplines (2)

Discipline
Immunology, Immunopathology
Experimental Cancer Research

Keywords (3)

multidimensional single cell analysis; mass cytometry; Hyperion

Lay Summary (German)

Lead
Komplexe Krankheiten, wie z.B. Krebserkrankungen, weisen in der Regel eine große Anzahl genetischer und/oder epigenetischer Veränderungen in verschiedenen Zelltypen auf. Diese Veränderungen können von unterschiedlicher pathologischer und therapeutischer Bedeutung sein. Die neue Plattform leistet einen Beitrag zum Entdecken von diesen Veränderungen.
Lay summary

Inhalt und Ziele des Forschungsprojekts

Für die Umsetzung der Präzisionsmedizin ist das Erkennen und Verstehen, ob und wie solche Veränderungen die zelluläre Dysfunktion beeinflussen, zwingend. Technologien, welche es erlauben, derartige Veränderungen auf Einzelzellbasis zu untersuchen, erbringen einen hohen Nutzen in der Aufklärung solcher komplexen biologischen Phänomene. Darüber hinaus werden derartige Einzelzellmessungen für das Studium der Krankheitsbiologie routinemäßig unverzichtbar und sind zielführend für zukünftige klinische Anwendungen wie z.B. die Identifizierung von neuen Biomarkern.

Die geplante Anschaffung dient zur Etablierung einer Plattform zur hochdimensionalen Analyse von Einzelzellen mittels bildgebender Massenzytometrie, namentlich der HyperionTMImaging Plattform. Diese Plattform ermöglicht eine multiparametrische zelluläre Analyse und das Studium von komplexen zellulären Strukturen in Zellpopulationen und deren Interaktion mit Gewebezellen.

Wissenschaftlicher und gesellschaftlicher Kontext des Forschungsprojekts

Wir sind überzeugt, dass diese neue Plattform den Forschenden der Universität Bern und des Inselspitals den Zugang zu einem Instrument der nächsten Generation ermöglicht, welcher das Erforschen komplexer Pathologien und das Studium der interzellulären Kommunikation in kombinierter Form von chemischen und räumlichen Informationen erlaubt.

 

Direct link to Lay Summary Last update: 21.09.2019

Responsible applicant and co-applicants

Project partner

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Abstract

Complex diseases commonly involve large numbers of genetic or epigenetic changes across several cell types, each of variable pathologic and therapeutic relevance. Identifying and understanding how such alterations collectively drive cellular dysfunction is a mandatory requirement in the implementation of precision medicine and requires complex technological efforts along with state-of-the-art computational tools. In that respect, single-cell technologies are entering the mainstream, providing an important utility to elucidate a diverse range of complex biological phenomena. Moreover, single-cell measurements are routinely becoming indispensable for the study of disease biology since heterogeneous cell populations undergo dynamic changes, differentiating toward many distinct identities a fact that has a key role in adjusting the appropriate therapies. This project will install a Hyperion CyTOF Imaging System that will enable comprehensive analysis of cellular phenotypes and their interrelationships through Imaging Mass Cytometry. This platform will be used to measure multiple protein markers in the spatial context of the tissue microenvironment and in single cell suspensions, gain full microenvironment and tissue architecture data, run panels of over 40 detection parameters, and avoid non-specific signals obtained from fluorescence reporter overlap.This new platform instrument will provide the researchers at the University of Bern with a next generation state-of-the-art tool to study complex disease biology and to delve into intercellular communication with a higher degree of combined chemical and spatial information.
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