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Respiratory disease Occupational Biomonitoring Collaborative Project (ROBoCoP)

English title Respiratory disease Occupational Biomonitoring Collaborative Project (ROBoCoP)
Applicant Guseva-Canu Irina
Number 177067
Funding scheme COST (European Cooperation in Science and Technology)
Research institution Policlinique Médicale Universitaire PMU
Institution of higher education University of Lausanne - LA
Main discipline Respiratory Diseases
Start/End 01.05.2018 - 30.04.2022
Approved amount 319'998.00
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All Disciplines (2)

Respiratory Diseases
Chemical Engineering

Keywords (11)

prospective cohort ; oxidative potential ; field study; analytical chemistry ; chronic obstructive pulmonary disease; exhaled breath condensate (EBC); biomarker; exhaled air ; photonic amplification; occupational health; particles

Lay Summary (French)

Respiratory disease Occupational Biomonitoring Collaborative Project (ROBoCoP)
Lay summary

La prévention des effets respiratoires liés aux expositions professionnelles à des aérocontaminants est un enjeu important pour les médecins du travail. Entre 20 et 30% des bronchites chroniques obstructives (BPCO) sont liées à des expositions professionnelles (particules fines ou ultrafines) inductrices d’un stress oxydant. Des biomarqueurs du stress oxydant pourraient être proposés pour le dépistage précoce des effets respiratoires, notamment de la BPCO, à condition d’être validés et mesurables dans des prélèvements biologiques peu invasifs.

Le projet ROBoCop est bâti dans le cadre de l’Action EU-COST CA16113 « cliniMARK », en collaboration avec l’Unité de prévention en santé au travail de la RATP, France.  Il comporte trois parties :

1-Standardisation du recueil des échantillons biologiques (condensat d’air exhalé, urine) et des méthodes d’analyses des biomarqueurs retenus (8-isoprostane, malondialdehyde, NO2-, NO3-, ions formates et 8-hydroxy-2’deoxyguanosine) et validation de l’analyseur OPEA (oxidative potential in the exhaled air) développé par l’IST (Brevet EU et USA).

2-Etude pilote à la RATP pour définir le protocole d’échantillonnage standardisé et évaluer la faisabilité de la mesure des biomarqueurs sélectionnés chez les travailleurs exposés aux particules.

3-Etude de terrain sur 300 travailleurs volontaires de la RATP, sélectionnés par randomisation stratifiée sur les 45’000 travailleurs suivis par les 34 médecins de l’entreprise. Les données issues des consultations médicales et de l’analyse des échantillons biologiques seront analysées afin de rechercher une corrélation entre les biomarqueurs étudiés et la survenue d’un syndrome obstructif.

Ce projet permettra d’élaborer des protocoles standardisés et des recommandations de bonne pratique pour l’usage des biomarqueurs du stress oxydatif à partir des prélèvements non invasifs, dans un service de santé au travail. Il permettra de valider l’utilisation de biomarqueurs pour le dépistage de la BPCO ou pour le suivi médical de patients atteints par cette pathologie.

Direct link to Lay Summary Last update: 16.04.2018

Responsible applicant and co-applicants


Name Institute


Urinary 8-isoprostane as a biomarker for oxidative stress. A systematic review and meta-analysis
Graille M., Wild P., Sauvain J-J, Hemmendinger M., Guseva Canu I., Hopf N.B. (2020), Urinary 8-isoprostane as a biomarker for oxidative stress. A systematic review and meta-analysis, in Toxicology Letters, 328, 19-27.
Reference ranges of oxidative stress biomarkers selected for non-invasive biological surveillance of nanotechnology workers: Study protocol and meta-analysis results for 8-OHdG in exhaled breath condensate
Hemmendinger M., Wild P., Shoman Y., Graille M., Bergamaschi E., Hopf N., Guseva Canu I. (2020), Reference ranges of oxidative stress biomarkers selected for non-invasive biological surveillance of nanotechnology workers: Study protocol and meta-analysis results for 8-OHdG in exhaled breath condensate, in Toxicology Letters, 327, 41-47.
Does exposure to inflammatory particles modify the pattern of anion in exhaled breath condensate?
Sauvain J-J, Edmé J-L, Wild P, Suarez G, Bezerra O M P A, Talvani A, Algranti E, Carneiro A P S, Chérot-Kornobis N, Sobaszek A, Hulo S (2020), Does exposure to inflammatory particles modify the pattern of anion in exhaled breath condensate?, in Journal of Breath Research, 14(2), 026005-026005.


Group / person Country
Types of collaboration
Université de Lille, Laboratoire EA 2694, Santé Publique : Epidémiologie et Qualité des soins France (Europe)
- Research Infrastructure
- Exchange of personnel
Régie autonome de transport parisien (RATP) France (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Exchange of personnel
- Industry/business/other use-inspired collaboration
Institute for Medical Research and Occupational Health Croatia (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Research Infrastructure
Laboratory of Toxicology and Industrial Epidemiology, University of Turin Italy (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
Colloque national d’épidémiologie en santé au travail Poster Assessment of determinants of occupational exposure to particulate matter in the underground railway areas and resulting oxidative stress 14.11.2019 Toulouse, France Pralong Jacques; Hopf Nancy; Wild Pascal Pierre; Guseva-Canu Irina; Hemmendinger Maud; Sauvain Jean-Jacques; Suarez Guillaume;
Colloque national d’épidémiologie en santé au travail Poster Study of inter- and intra-individual variability in the measurement of oxidative stress biomarkers for validation for biomonitoring in environmental and occupational health 14.11.2019 Toulouse, France Sauvain Jean-Jacques; Guseva-Canu Irina; Suarez Guillaume; Hemmendinger Maud;
international conference Environmental and Occupational Health Aspects Related to Nano- and Ultrafine Particulate Matter Poster Non-invasive sensitive method for oxidative stress quantification in exhaled air 05.06.2019 Loen, Norway Sauvain Jean-Jacques;
5th CliniMark meeting - workshop Talk given at a conference Optimization of methods for the detection of oxidative stress markers in exhaled air 29.03.2019 Basel, Switzerland Guseva-Canu Irina; Hemmendinger Maud; Suarez Guillaume;
National congress of occupational medicine Poster Evaluation de l’intérêt des biomarqueurs dans le suivi des travailleurs exposés 05.06.2018 Marseille, France Rinaldo Michaël;


Title Date Place

Knowledge transfer events


Title Date Place


The project is prepared within the framework of CA16113 “CliniMARK” focused on chronic obstructive pulmonary disease (COPD) biomarker development and validation. COPD is a major and increasing global health problem. In Europe, the prevalence of COPD is estimated to be 13.7% with an annual burden of €38.7 billion. In Switzerland, COPD prevalence is particularly high, reaching 15.9% in men and 19.0% in both gender smokers. Smoking but also outdoor air pollution and many industrial activities generate particulate matter (PM), a known COPD risk factor. Via oxidative stress pathway, PM produces reactive oxygen species (ROS). ROS and their metabolites could be informative with regards to the disease development and thus be measured as diagnostic and companion diagnostic biomarkers. Among selected oxidative stress biomarkers, that can be measured non-invasively such as in exhaled breath condensate and urine (8-isoprostane, malondialdehyde, NO2-, NO3-, formate ion, and 8-hydroxy-2’deoxyguanosine); we will identify those associated with COPD diagnosis. In addition, we will evaluate oxidative potential in the exhaled air (OPEA) that will be measured with our OPEA analyzer (EU Patent). We will validate the use of this analyzer in the field, at a Parisian Transport Company (RATP). The research is divided into three work-packages (WP) with the following specific activities:WP1-All biological sampling procedures and biomarkers detection techniques will be standardized and submitted for CliniMARK approval WP2-A pilot study will determine the best time to collect samples and assess the suitability of the selected biomarkers with short half-life in surveying populations at risk (RATP workers exposed to PM). The Field study protocol will be reviewed by CliniMARK WP3-A field study will be conducted among 300 RATP workers selected by stratified randomization from the prospective cohort (45’000 workers). Workers will be enrolled and undergo medical examination (lung function test) where biological samples will be collected. Biological samples will be analyzed as received and a database of results will be built. Quality control will be assured while sampling exhaled air and measuring OPEA. After the sample analyses are finished, health and biomarker data will be merged and analyzed to determine the associations of one or a panel of the selected biomarkers, including OPEA, with obstructive syndrome (FEV1/FVC<70%).In conclusion, we will have addressed the validity of the tested biomarkers with respect to COPD diagnosis. We will have demonstrated the feasibility of standardized protocols and biomarkers, as well as best practices guidelines when measuring OPEA and other biomarkers in the occupational setting, using non-invasively collected samples. The validated biomarkers will thus be useful in screening large populations at risk and cost-effectively tailoring primary and secondary prevention of COPD. It will also be useful in monitoring COPD evolution and severity.