Project

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Role of maternal microbiota and aryl hydrocarbon receptor signalling in establishing neonatal skin homeostasis

Applicant Gomez de Agüero Tamargo Maria de la Mercedes
Number 168012
Funding scheme Ambizione
Research institution Department for BioMedical Research Abteilung Gastroenterologie Universität Bern
Institution of higher education University of Berne - BE
Main discipline Immunology, Immunopathology
Start/End 01.01.2017 - 31.12.2019
Approved amount 598'901.00
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All Disciplines (2)

Discipline
Immunology, Immunopathology
Medical Microbiology

Keywords (7)

Maternal microbiota; skin; aryl hydrocarbon receptor; Neonate; Keratinocytes; Langerhans cells; Epidermal invariant gammadelta T cells

Lay Summary (French)

Lead
La peau est le plus large organe de notre corps au contact de l’environnement. Dès la naissance, elle constitue une barrière physique et immunologique qui nous protège en même temps qu’elle abrite la flore bactérienne. La préparation de cette solide et en même temps, douillette forteresse pendant le développement fœtal serait orchestré par le microbiome maternelle en dépit d’une programmation génétique. Le projet essayera d’éclaircir cette question.
Lay summary

La peau, constituée de nombreuses couches de keratinocytes et d’un réseau de cellules du système immunitaire, forme une barrière qui nous protège activement des agents de l’environnement en même temps qu’elle héberge des millions de microbes. Le développement de la peau se déroule pendant la grossesse pour préparer le nouveau-né au challenge de la colonisation massive qui se produit à la naissance. La flore maternelle qui est responsable de la préparation de la barrière intestinale pourrait être impliquée aussi dans le développement de la peau.

 

Dans un premier temps, nous étudierons le rôle du microbiome maternel dans le développent des cellules de la peau, keratinocytes et cellules du système immunitaire tels que les cellules de Langerhans et les lymphocytes T gamma-delta.

Ensuite, nous analyserons les contributions relatives des composant de la flore maternelle, de la diète ou de facteurs endogènes dans le développement des constituants de la peau via l’activation du récepteur de l’aryl hydrocarbon.

Pour finir, nous explorerons la contribution de la flore maternelle dans la fonction de barrière de la peau dans différentes situations naturelles tels que la colonisation post-natale, la tolérance aux produits chimiques inoffensifs ou dans le processus de cicatrisation. 

Direct link to Lay Summary Last update: 05.03.2018

Responsible applicant and co-applicants

Employees

Publications

Publication
Standardization in host–microbiota interaction studies: challenges, gnotobiology as a tool, and perspective
Mooser Catherine, Gomez de Agüero Mercedes, Ganal-Vonarburg Stephanie C (2018), Standardization in host–microbiota interaction studies: challenges, gnotobiology as a tool, and perspective, in Current Opinion in Microbiology, 44, 50-60.
Maternal microbiota and antibodies as advocates of neonatal health
Mercedes Gomez de Aguero, Stephanie Ganal-Vonarburg, Tobias Fuhrer (2017), Maternal microbiota and antibodies as advocates of neonatal health, in Gut Microbes, 8(5), 479-485.

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
2nd Young scientific meeting in Bern Poster Maternal microbiota drives B cell development in the fetal liver 09.01.2019 Bern, Switzerland Gomez de Agüero Tamargo Maria de la Mercedes;
“Technical and operational advances in gnotobiology symposium” Talk given at a conference Experimental use of axenic and gnotobiotic animals: isolators and sealed IVC system 15.11.2018 Geneve, Switzerland Gomez de Agüero Tamargo Maria de la Mercedes;
SGG Annual congress Talk given at a conference Maternal microbiota drive B cell development in neonatal liver 26.09.2018 Interlaken, Switzerland Gomez de Agüero Tamargo Maria de la Mercedes;
EAACI congress Talk given at a conference The maternal microbiota drives early postnatal innate immune development 26.05.2018 Munich, Germany Gomez de Agüero Tamargo Maria de la Mercedes;
First 1000 days congress Talk given at a conference The maternal microbiota drives early postnatal innate immune development 14.03.2018 Anthalya, Turkey Gomez de Agüero Tamargo Maria de la Mercedes;
Gut Inflammation Talk given at a conference Maternal microbiota sets neonatal immune system 18.05.2017 Ghent, Belgium Gomez de Agüero Tamargo Maria de la Mercedes;


Communication with the public

Communication Title Media Place Year
Print (books, brochures, leaflets) Maternal Microbiota Impacts Offspring Immune System International 2018

Awards

Title Year
Poster prize from 2nd Young Scientists Meeting in Bern. Jan-2019. "Neonatal skin barrier is shaped by embryonic exposure to maternal microbiota" Received by Md-PhD student Anna Wenning. 2019

Abstract

The skin constitutes a physical and immunological barrier protecting the body against foreign antigens and pathogens, whilst at the same time being home to a commensal microbial community. Mammalians born germ-free get colonized by millions of microorganism at the time of the birth. A fundamental equilibrium is established in the skin between the immune system and the microbiota in order to set up the tissue homeostasis protecting against the colonization of the systemic part of the body by the microorganisms and aberrant immune responses. Recently, several studies have defined a critical window during the prenatal and neonatal life for the microbiota to shape the immune system in order to establish tissue baseline in the gut or systemically. The mechanism of the education of the immune system by the microbiota has been largely studied in the gut, however remains to be elucidated in the skin. The development of the epidermal cells, such as keratinocytes, Langerhans cells and epidermal invariant gd T cells, is exclusively intra-uterine from fetal progenitor. This altricial ontogeny together with their innate capacity to respond to antigens opens the possibility that they make an important contribution to the neonatal establishment of microbiota mutualism challenging the dogma of a genetic pre-programming of these immune cells function. The aim of the project is to determine if maternal microbiota molecular transfer acts during a critical window before and after birth independently of an endogenous microbiota and the genetique preprogramming and shapes the skin immune system development of the offspringUnderstanding the role of maternal microbiota in the establishment of skin ontogeny and identifying the mediators are crucial to understand the defect in the cutaneous homeostasis that must occur in the initiation of inflammatory skin diseases or infection. My project will contribute to the conceptual development of potential new therapeutic tools capable of boosting local immunity while preserving tissue homeostasis.
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