Dockx Greet, Geisel Steffen, Moore David G., Koos Erin, Studart Andre R., Vermant Jan (2018), Designer liquid-liquid interfaces made from transient double emulsions, in Nature Communications
, 9(1), 4763-4763.
Beltramo Peter J., Scheidegger Laura, Vermant Jan (2018), Toward Realistic Large-Area Cell Membrane Mimics: Excluding Oil, Controlling Composition, and Including Ion Channels, in Langmuir
, 34(20), 5880-5888.
Beltramo Peter J., Gupta Manish, Alicke Alexandra, Liascukiene Irma, Gunes Deniz Z., Baroud Charles N., Vermant Jan (2017), Arresting dissolution by interfacial rheology design, in Proceedings of the National Academy of Sciences
, 114(39), 10373-10378.
Pepicelli Martina, Verwijlen Tom, Tervoort Theo A., Vermant Jan (2017), Characterization and modelling of Langmuir interfaces with finite elasticity, in Soft Matter
, 13(35), 5977-5990.
Beltramo Peter Vermant Jan (2016), Simple Optical Imaging of Nanoscale Features in Free-Standing Films, in ACS omega
, 1(3), 363-370.
RenngliDamian, AlickeAlexandra, EwoldtRandy, VermantJan, Operating windows for oscillatory interfacial shear rheology", in Journal of Rheology
Soft matter interfaces are common to living systems, foods, personal products, and the environment. They occur whenever surface-active moieties or particles find themselves at fluid interfaces and render them non-linear in their response to flow and deformation. In the last decades good progress has been made in the experimental characterization of monolayers of surface-active species or particles. For bilayers, which constitute the membrane of living cells and can be viewed as the ultimate success of self-assembly in materials science, the situation is different and despite the even greater relevance of such systems our current understanding is limited. In this project we propose take on the challenge to measure and characterize the full interfacial rheology-structure relation of bilayers. So far, this was difficult as existing methods to fabricate freestanding model membranes are limited in size and control over composition and thermodynamics and thus the studies that can be performed are limited. We have developed a method to generate freestanding, planar, phospholipid bilayers with millimeter scale areas. The technique relies on an adapted thin-film balance apparatus allowing for the dynamic control of the nucleation and growth of a planar black lipid membrane in the center of an orifice surrounded by microfluidic channels. Success has been demonstrated using several different lipid types, including mixtures that show the same temperature dependent phase separation as existing protocols. An advantage unique to our approach is the dynamic control of the membrane tension. We now propose to use this platform for freestanding, planar, phospholipid bilayers with millimeter scale areas to measure the rheological properties of these systems, trying to obtain well-defined kinematic conditions for shear, dilation and bending. The goal is to come to a complete holistic description of the membrane rheology. This should help us understand these systems, rationalize their mechano-chemical response, and lead to a new paradigm for studying the mechanics, structure, and function of model membranes.