Project

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United we stand divided we fall: Chlamydomonas reinhardtii strategy to endure micropollutant toxicity

Applicant Cheloni Giulia
Number 164428
Funding scheme Marie Heim-Voegtlin grants
Research institution Institut F.-A. Forel Université de Genève
Institution of higher education University of Geneva - GE
Main discipline Environmental Research
Start/End 01.07.2016 - 30.11.2018
Approved amount 195'283.00
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All Disciplines (2)

Discipline
Environmental Research
Other disciplines of Environmental Sciences

Keywords (6)

adaptive stress responses; Chlamydomonas reinhardtii; palmelloid; micropollutant; phenotypic plasticity; evolution of multicellularit

Lay Summary (French)

Lead
Pour faire face à des conditions défavorables, ou en présence de facteurs de stress environnementaux, les organismes vivants peuvent activer des réponses adaptatives. La plasticité phénotypique est une stratégie couramment utilisée par les organismes qui doivent faire face à des changements de leur environnement. Cependant la plasticité phénotypique en réponse à la présence de micropolluants toxiques reste très mal connue. Ce projet portera spécifiquement sur la capacité de l'algue verte unicellulaire Chlamydomonas reinhardtii à former des colonies (palmelloïdes) en présence de concentrations sub-létales de micropolluants.
Lay summary

Contenu et objectifs du travail de recherche

 L'objectif du projet est d'étudier la formation de colonies palmelloïdes comme stratégie pour faire face à la toxicité de micropolluants. La formation des colonies sera étudiée après exposition à des micropolluants ayant différents modes d'action toxique. Les mécanismes qui entraînent le passage de l'unicellularité au mode de vie colonial seront étudiés au niveau cellulaire, physiologique et génétique à l'aide de multiples techniques, empruntées à différentes disciplines.

 Contexte scientifique et social du projet de recherche

 Les résultats de ce projet seront pertinents aussi bien au niveau des sciences fondamentales que de leur application. Spécifiquement, cette recherche apportera une contribution dans le domaine de l'écotoxicologie en permettant une meilleure estimation du potentiel toxique des micropolluants envers les algues eucaryotes, qui sont des acteurs clés dans le fonctionnement des écosystèmes aquatiques. L'étude pourrait aussi avoir de profondes implications pour la compréhension de l'évolution de la multicellularité dans le règne végétal.

 

Direct link to Lay Summary Last update: 02.06.2016

Responsible applicant and co-applicants

Employees

Publications

Publication
Combined Effects of Trace Metals and Light on Photosynthetic Microorganisms in Aquatic Environment
Cheloni Giulia, Slaveykova Vera (2018), Combined Effects of Trace Metals and Light on Photosynthetic Microorganisms in Aquatic Environment, in Environments, 5(7), 81-81.

Collaboration

Group / person Country
Types of collaboration
Sabeeha Merchant Lab United States of America (North America)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Goldschmidt-Clermont Lab Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
Science Mass Sprectrometry (SMS) platform, UniGe Switzerland (Europe)
- Research Infrastructure
Neuchâtel Platform of Analytical Chemistry (NPAC) Switzerland (Europe)
- Research Infrastructure

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
18th INTERNATIONAL CONFERENCE ON THE CELL AND MOLECULAR BIOLOGY OF CHLAMYDOMONAS Poster United we Stand Divided We Fall: Chlamydomonas Strategy to Endure Micropollutant Toxicity 17.06.2018 Washington DC, United States of America Cheloni Giulia;
EcotoxicoMic conference Talk given at a conference Morphological plasticity in Chlamydomonas reinhardtii: an adaptive response to micropollutant stress 21.11.2017 Lyon, France Cheloni Giulia;
ICOBTE conference Talk given at a conference Inter-species interactions alter copper toxicity to aquatic phytoplankton 16.07.2017 Zurich, Switzerland Cheloni Giulia;
Invited seminar EAWAG Individual talk The role of light irradiation and algal-algal interactions on the toxicity of copper based micropollutants to Chlamydomonas reinhardtii 08.11.2016 Dübendorf, Switzerland Cheloni Giulia;
XXXIII SILCongress Talk given at a conference Phenotypic plasticity of Chlamydomonas reinhardtii: an adaptive response to copper stress 31.07.2016 Torino, Italy Cheloni Giulia;


Awards

Title Year
Ernst and Lucie Schmidheiny fellowship 2018
Plantamour-Prevost grant 2017
Fonds d’encouragement SSHL de la relève 2016

Associated projects

Number Title Start Funding scheme
183778 ESCAPE: strEss response pathways in Chlamydomonas reinhArdtii and PalmElloid formation 01.12.2018 Marie Heim-Voegtlin grants

Abstract

To resist and survive adverse environmental conditions microorganisms have evolved multiple strategies to maintain cellular homeostasis. Several adaptive responses were suggested for Chlamydomonas reinhardtii to face dynamic changes in environmental parameters such as light, temperature or nutrient availability, however still little is known about micropollutant induced stress response pathways. With this project we aim to elucidate the mechanisms of palmelloid colony formation as a cellular strategy to avoid micropollutant toxicity. Despite the deep knowledge of C. reinhardtii cell biology the mechanisms that drive the transition from unicellular to colonial lifestyle are currently unclear and the biological function of palmelloid formation is still in question. In our work a multidisciplinary approach will be adopted in which micropollutant induced palmelloid formation will be investigated at the genetic, cellular and physiological level. The process of palmelloid formation and its reversal will be characterized in presence of micropollutant with different modes of action under long term exposure. The influence of palmelloid formation on micropollutant uptake will be investigated. Genes potentially involved in C. reinhardtii transition to colonial lifestyle will be identified through selection of mutants and transcriptomics. The outcomes of this project are expected to have far reaching implication in the field of ecotoxicology and micropollutant risk assessment, as well as in studies concerning evolution of multicellularity in the plant kingdom.
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