body fluids; diabetes; mitochondrial function ; lipid quantification ; nephrology; metabolomics; metabolite profiling; biomarker identification; natural extracts; atherogenesis
Brillatz Théo, Lauritano Chiara, Jacmin Maxime, Khamma Supitcha, Marcourt Laurence, Righi Davide, Romano Giovanna, Esposito Francesco, Ianora Adrianna, Queiroz Emerson F., Wolfender Jean-Luc, Crawford Alexander D. (2018), Zebrafish-based identification of the antiseizure nucleoside inosine from the marine diatom Skeletonema marinoi, in PLOS ONE
, 13(4), e0196195-e0196195.
Olivon Florent, Allard Pierre-Marie, Koval Alexey, Righi Davide, Genta-Jouve Gregory, Neyts Johan, Apel Cécile, Pannecouque Christophe, Nothias Louis-Félix, Cachet Xavier, Marcourt Laurence, Roussi Fanny, Katanaev Vladimir L., Touboul David, Wolfender Jean-Luc, Litaudon Marc (2017), Bioactive Natural Products Prioritization Using Massive Multi-informational Molecular Networks, in ACS Chemical Biology
, 12(10), 2644-2651.
Allard Pierre-Marie, Genta-Jouve Grégory, Wolfender Jean-Luc (2017), Deep metabolome annotation in natural products research: towards a virtuous cycle in metabolite identification, in Current Opinion in Chemical Biology
, 36, 40-49.
Characterisation of metabolites is a must to deepen our outstanding of fundamental processes in various fields of life sciences. De novo identification of new bioactive molecules has been and remains the focus of natural product research and this also extends toward chemical biology. With the recent progress in systems biology and metabolomics, the pace of metabolite and biomarker characterisation has exponentially increased. The unambiguous identification of low molecular weight markers however represents still a major bottleneck in the ‘omics’ sciences.In this respect Nuclear Magnetic Resonance (NMR) is the reference method for correctly assessing a metabolites structure and its 3D configuration. It is the mandatory complement to HRMS/MS when databases are lacking for straightforward spectral MS/MS matches and is often mandatory to distinguish various types of isomers. In addition NMR is also now well established for robust profiling of complex mixtures and for de novo identification of key biomarkers. Based on the tremendous advances in NMR technology and sensitivity over the last two decades, the access to a high end high field NMR system (600 MHz) at the University of Geneva is strongly needed to acquire high resolution metabolite profiles of complex biological matrices and perform characterisation of low amounts of metabolites with high throughput.Within the School of Pharmaceutical Sciences the group of Prof. Wolfender has a renowned expertise in the de novo identification of complex natural products by NMR/MS, and metabolite profiling/metabolomics. He is presently sharing this expertise with the researchers involved in Pharmaceutical Sciences and other partners of the UNIGE as well as with researchers of the Lemanic area through various collaborative projects. In particular in the School of Biology important needs exist for complete and unambiguous identification of biomarkers present in low concentrations and isolated in small quantities. Partner at the School of Chemistry and Biochemistry will also need to access the new NMR for in depth structural characterisation and for quantification in lipidomics studies. The researchers of the Faculty of Sciences involved in metabolite profiling and characterisation studies have initiated joint projects with clinicians of the University of Geneva notably in the field of NMR metabolomics which is rapidly expanding at the clinical level. Taking advantage of a move of the School of Pharmaceutical Sciences in 2016 in new high technology laboratories of the CMU, they will have a perfect environment for expanding their interaction.The opportunity to have a new NMR in this medical surrounding will offer the unique possibility in Switzerland to perform NMR metabolomics application for clinicians on site and this will strengthen existing projects in particular in the groups of Prof Maechler, Vuilleumier, Martin and their colleagues. Furthermore this will create new avenues for innovative translational medicine projects integrating the NMR metabolomics aspects in good complementarity and partnership with the strong competences in clinical proteomics of the UNIGE.The proposed NMR acquisition at the University of Geneva will thus strengthen existing lines of research within the School of Pharmaceutical Sciences in natural products research metabolomics and biofluids’ profiling. It will considerably improve and strengthen collaborations with other groups with common needs within the Lemanic area and enable new lines of research in medical sciences between the Faculty of Medicine and the Faculty of Sciences. Various projects of researchers of the UNIGE with needs in complex metabolites profiling and biomarkers identification are described in details in this grant application.