Project

Back to overview

Identifying environmental controls over the transcriptional activity of single cells across organs and systems

English title Identifying environmental controls over the transcriptional activity of single cells across organs and systems
Applicant Jabaudon Denis
Number 164091
Funding scheme R'EQUIP
Research institution Dépt des Neurosciences Fondamentales Faculté de Médecine Université de Genève
Institution of higher education University of Geneva - GE
Main discipline Genetics
Start/End 01.12.2015 - 30.11.2016
Approved amount 267'682.00
Show all

All Disciplines (2)

Discipline
Genetics
Cellular Biology, Cytology

Keywords (6)

neuroscience; genetics; oncology; endocrinology; single cell biology; gene environment interactions

Lay Summary (French)

Lead
Le système Polaris qui fait l’objet de ce soutien permet d’analyser le transcriptome complet de cellules individuelles mises en culture et exposées à des modifications de l’environnement contrôlées. Il s’agit donc d’un outil de pointe pour la dissection des voies de signalisations moléculaires ainsi que l’analyse de réponses cellulaires à des composés pharmacologiques.
Lay summary

Le système Polaris qui fait l’objet de ce soutien permet d’analyser le transcriptome complet de cellules individuelles mises en culture et exposées à des modifications de l’environnement contrôlées. Il s’agit donc d’un outil de pointe pour la dissection des voies de signalisations moléculaires ainsi que l’analyse de réponses cellulaires à des composés pharmacologiques.Les projets impliqués concerneront les neuroscience, la génétique humaine, et l'endocrinologie. Le principe est d'identifier des voies de transductions dans chacun de ces systèmes avec une résolution cellulaire.

Direct link to Lay Summary Last update: 30.11.2015

Responsible applicant and co-applicants

Associated projects

Number Title Start Funding scheme
152873 Kinetic Analysis of S.aureus Biofilm Regulation by Using Combined Imaging and Genome-Wide Approaches 01.05.2014 Project funding (Div. I-III)
152965 ß-cell Reconstitution after ß-cell Ablation by Islet Cell Type Interconversion 01.04.2014 Project funding (Div. I-III)
141084 DNA damage checkpoint pathways 01.07.2012 Project funding (Div. I-III)
144082 Chromosome 21: functional genomics and molecular pathophysiology of its disorders 01.10.2012 Project funding (Div. I-III)
155896 Investigating the impact of the psychiatric-associated risk-genes on cortical circuit formation 01.10.2014 Project funding (Div. I-III)
149753 Emergence and regulation of vomeronasal receptor genes in the mouse 01.10.2013 Project funding (Div. I-III)
157846 Self-Organizing Properties of Neocortical Circuits 01.04.2015 Temporary Backup Schemes
153410 Does ensemble decorrelation in the olfactory bulb predict odor discrimination behavior? 01.04.2014 Project funding (Div. I-III)
152636 The role of the IGF system in testis development, spermatogenesis and endocrine functions in mice 01.04.2014 Project funding (Div. I-III)
149984 Population and individualized transcriptome analysis 01.10.2013 Project funding (Div. I-III)

Abstract

The anatomical and functional organization of cells within distinct organs results from interactions be-tween cell-intrinsic genetic factors and cell-extrinsic environmental factors. While recent progress has been made in characterizing and understanding cell-intrinsic transcriptional programs in many organs and conditions, even in single cells, how the environment controls the transcriptional activity and overall identity of cells across organs has been difficult to systematically investigate. A major limitation to-wards this aim has been the inability to perturb the environment of single cells and identify stimulus-evoked transcriptional responses in controlled conditions. The state-of-the-art device that we propose to acquire in the frame of this proposal is designed for such a purpose: the Polaris System (Fluidigm Inc) allows integrated single-cell isolation, culture and mo-lecular preparation into a single workflow, such that single cells from a given tissue can be isolated, acutely or chronically challenged/stimulated, and immediately analyzed for gene expression pattern. It builds on previous generation of single-cell sorting devices, which are limited to simple transcriptional characterization, by allowing controlled environmental manipulation and characterization of evoked tran-scriptional responses. The Polaris system therefore bridges the gap between cell-intrinsic and cell-extrinsic transcriptional programs by allowing access to the intracellular transcriptional pathways activat-ed by controlled environmental stimuli.
-