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Automated Peptide Purification System

English title Automated Peptide Purification System
Applicant Reymond Jean-Louis
Number 164025
Funding scheme R'EQUIP
Research institution Departement für Chemie und Biochemie Universität Bern
Institution of higher education University of Berne - BE
Main discipline Organic Chemistry
Start/End 01.01.2016 - 31.12.2016
Approved amount 45'462.00
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Keywords (8)

Cardiac ion channels; High-Throughput Purification; Automated Peptide Synthesis; Antimicrobials; Preparative HPLC; Chemical Space; Drug conjugates; Peptides

Lay Summary (German)

Lead
Präparative HPLC mit Autosampler für Pepitd Reinigung
Lay summary
Unsere Forschungsgruppe entwickelt Peptide mit neuartigen Topologie, insbesondere mehrfach verzweigte Peptide (Dendrimere und Polyzyklen) für therapeutische Zwecke, mit Schwergewicht auf die Entwicklung neuartiger Antibiotika gegen resistente Pathogen, sowohl als auch Peptide als Proben im Rahmen verschiedener Zusammenarbeitsprojekten an der Universität Bern. Um unser Durschsatz zu erhöhen haben wir letztes Jahr zwei Mikrovwellen Peptid Synthesizer angeschafft. Diese Roboter erlauben uns, voll automatisierte Synthesen durchzuführen. Zur Reinigung der vielen Produkten aus diesen automatisierten Synthesen brauchen wir einpräparatives Säulenchromatographie System, welches ebenfalls automatisch arbeitet. Somit können wir unsere Produktivität erhöhen und bessere Peptid produkte schneller entwickeln
Direct link to Lay Summary Last update: 15.04.2016

Responsible applicant and co-applicants

Publications

Publication
Anti-Microbial Dendrimers against Multidrug-Resistant P. aeruginosa Enhance the Angiogenic Effect of Biological Burn-wound Bandages
Abdel-Sayed Philippe, Kaeppeli Ariane, Siriwardena Thissa, Darbre Tamis, Perron Karl, Jafari Paris, Reymond Jean-Louis, Pioletti Dominique P., Applegate Lee Ann, Anti-Microbial Dendrimers against Multidrug-Resistant P. aeruginosa Enhance the Angiogenic Effect of Biological Burn-wound Bandages, in Scientific Reports, 2016, 22020.
Efficient Transfection of siRNA by Peptide Dendrimer–Lipid Conjugates
Kwok Albert, Eggimann Gabriela A., Heitz Marc, Reymond Jean-Louis, Hollfelder Florian, Darbre Tamis, Efficient Transfection of siRNA by Peptide Dendrimer–Lipid Conjugates, in ChemBioChem, 17(23), 2223-2229.
Multivalency effects on Pseudomonas aeruginosa biofilm inhibition and dispersal by glycopeptide dendrimers targeting lectin LecA.
Bergmann M, Michaud G, Visini R, Jin X, Gillon E, Stocker A, Imberty A, Darbre T, Reymond JL, Multivalency effects on Pseudomonas aeruginosa biofilm inhibition and dispersal by glycopeptide dendrimers targeting lectin LecA., in Organic & Biomolecular Chemistry, 2016(14), 138-148.
Overcoming antibiotic resistance in Pseudomonas aeruginosa biofilms using glycopeptide dendrimers.
Michaud G., Visini R., Bergmann M., Salerno G., Bosco R., Gillon E., Richichi B., Nativi C., Imberty A., Stocker A., Darbre T., Reymond JL, Overcoming antibiotic resistance in Pseudomonas aeruginosa biofilms using glycopeptide dendrimers., in Chemical Science, 2016(7), 166-182.

Collaboration

Group / person Country
Types of collaboration
Mingqiang Qiao / Nankai University China (Asia)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Markus Aebi/ETHZ Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Roland Pieters / University of Utrecht Netherlands (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Christian van Delden / HUG Genève Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Exchange of personnel

Associated projects

Number Title Start Funding scheme
159941 A Chemical Space Approach to Bioactive Peptides 01.04.2015 Project funding (Div. I-III)
167048 Antimicrobial peptide dendrimers (AMPD) and bicyclic peptides (AMBP) as therapeutic agents against multidrug resistant bacteria 01.03.2017 NRP 72 Antimicrobial Resistance
155982 Controlling multi-antibiotic resistant bacteria with antimicrobial peptide dendrimers (AMPD) 01.10.2015 Bilateral programmes
147632 Transglyco: Chemistry, Enzymology and Physiology of Oligosaccharyltransferase 01.11.2013 Sinergia

Abstract

Our research projects heavily rely on the high-throughput synthesis of structurally diverse peptides. These projects include the exploration of the chemical space of topologically diverse peptides, the development of transfection agents and antimicrobials, the development of Ruthenium peptide conjugates as a novel type of anti-cancer agents, and the study of the regulation of cardiac ion channels. These projects require the ability to synthesize tens to hundreds of pure peptides and peptide constructs in a matters of days or weeks. To achieve this goal, we have recently acquired an automatic microwave peptide synthesizer (CEM Liberty Blue), which makes it possible to complete a peptide synthesis automatically with 2-5 hours, which is much faster and gives significantly higher yields than with room-temperature synthesis. This has raised our requirements for peptide purification to increase throughput. The present proposal is asking for support of a new automated peptide purification infrastructure in form of a preparative HPLC with an integrated auto-sampler, and a lyophilizer to concentrate pure fractions. These elements will replace an older manually operated preparative HPLC and complement an already existing automated preparative HPLC system, to make it possible to exploit our newly acquired peptide synthesis capacity.
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